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  • 1985-1989  (7)
  • 1975-1979  (4)
  • 1
    Digitale Medien
    Digitale Medien
    Analysis of bis(trimethylsilyl)acetamide for purity by proton magnetic resonance spectrometry (1979)
    s.l. : American Chemical Society
    Analytical chemistry 51 (1979), S. 311-313 
    Details
    ISSN: 1520-6882
    Quelle: ACS Legacy Archives
    Thema: Chemie und Pharmazie
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1021/ac50038a042
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Poly(dimethylsiloxane) as a reference standard for exact mass measurement in chemical ionization mass spectrometry (1987)
    s.l. : American Chemical Society
    Analytical chemistry 59 (1987), S. 194-197 
    Details
    ISSN: 1520-6882
    Quelle: ACS Legacy Archives
    Thema: Chemie und Pharmazie
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1021/ac00128a040
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  • 3
    Digitale Medien
    Digitale Medien
    Calcium ion dependency of ethylene production in segments of primary roots of Zea mays (1986)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 67 (1986), S. 0 
    Details
    ISSN: 1399-3054
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: We investigated the effect of Ca2+ on ethylene production in 2-cm long apical segments from primary roots of corn (Zea mays L., B73 × Missouri 17) seedlings. The seedlings were raised under different conditions of Ca2+ availability. Low-Ca and high-Ca seedlings were raised by soaking the grains and watering the seedlings with distilled water or 10 mM CaCl2, respectively. Segments from high-Ca roots produced more than twice as much ethylene as segments from low-Ca roots. Indoleacetic acid (IAA; 1 μM) enhanced ethylene production in segments from both low-Ca and high-Ca roots but auxin-induced promotion of ethylene production was consistently higher in segments from high-Ca roots. Addition of I-aminocyclopropane-I-carboxylic acid (ACC) to root segments from low-Ca seedlings doubled total ethylene production and the rate of production remained fairly constant during a 24 h period of monitoring. In segments from high-Ca seedlings ACC also increased total ethylene production but most of the ethylene was produced within the first 6 h. The data suggest that Ca2+ enhances the conversion of ACC to ethylene. The terminal 2 mm of the root tip were found to be especially important to ethylene biosynthesis by apical segments and, experiments using 45Ca2+ as tracer indicated that the apical 2 mm of the root is the region of strongest Ca2+ accumulation. Other cations such as Mn2+, Mg2+, and K+ could largely substitute for Ca2+. The significance of these findings is discussed with respect to recent evidence for gravity-induced Ca2+ redistribution and its relationship to the establishment of asymmetric growth during gravitropic curvature.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1399-3054.1986.tb05057.x
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  • 4
    Digitale Medien
    Digitale Medien
    The influence of calcium and pH on growth in primary roots of Zea mays (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 72 (1988), S. 0 
    Details
    ISSN: 1399-3054
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: Hasenstein, K. H. and Evans, M. L. 1988. The influence of calcium and pH on growth in primary roots of Zea mays. - Physiol. Plant. 72: 466–470.We investigated the interaction of Ca2+ and pH on root elongation in Zea mays L. cv. B73 × Missouri 17 and cv. Merit. Seedlings were raised to contain high levels of Ca2+ (HC, imbibed and raised in 10 mM CaCl2) or low levels of Ca2+ (LC, imbibed and raised in distilled water). In HC roots, lowering the pH (5 mM MES/Tris) from 6.5 to 4.5 resulted in strong, long-lasting growth promotion. Surprisingly, increasing the pH from 6.5 to 8.5 also resulted in strong growth promotion. In LC roots acidification of the medium (pH 6.5 to 4.5) resulted in transient growth stimulation followed by a gradual decline in the growth rate toward zero. Exposure of LC roots to high pH (pH shift from 6.5 to 8.5) also promoted growth. Addition of EGTA resulted in strong growth promotion in both LC and HC roots. The ability of EGTA to stimulate growth appeared not to be related to H+ release from EGTA upon Ca2+ chelation since, 1) LC roots showed a strong and prolonged response to EGTA, but only a transient response to acid pH, and 2) promotion of growth by EGTA was observed in strongly buffered solutions. We also examined the pH dependence of the release of 45Ca2+ from roots of 3-day-old seedlings grown from grains imbibed in 45Ca2+. Release of 45Ca2+ from the root into agar blocks placed on the root surface was greater the more acidic the pH of the blocks. The results indicate that Ca2+ may be necessary for the acid growth response in roots.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1399-3054.1988.tb09152.x
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  • 5
    Digitale Medien
    Digitale Medien
    Chemotaxis in photosynthetic bacteria (1979)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 6 (1979), S. 0 
    Details
    ISSN: 1574-6968
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1574-6968.1979.tb04286.x
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  • 6
    Digitale Medien
    Digitale Medien
    Analysis of growth patterns during gravitropic curvature in roots ofZea mays by use of a computer-based video digitizer (1986)
    Springer
    Journal of plant growth regulation 5 (1986), S. 73-83 
    Details
    ISSN: 1435-8107
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract A computer-based video digitizer system is described which allows automated tracking of markers placed on a plant surface. The system uses customized software to calculate relative growth rates at selected positions along the plant surface and to determine rates of gravitropic curvature based on the changing pattern of distribution of the surface markers. The system was used to study the time course of gravitropic curvature and changes in relative growth rate along the upper and lower surface of horizontally-oriented roots of maize (Zea mays L.). The growing region of the root was found to extend from about 1 mm behind the tip to approximately 6 mm behind the tip. In vertically-oriented roots the relative growth rate was maximal at about 2.5 mm behind the tip and declined smoothly on either side of the maximum. Curvature was initiated approximately 30 min after horizontal orientation with maximal (50°) curvature being attained in 3 h. Analysis of surface extension patterns during the response indicated that curvature results from a reduction in growth rate along both the upper and lower surfaces with stronger reduction along the lower surface.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02025958
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  • 7
    Digitale Medien
    Digitale Medien
    Nonselective ionic channels inAplysia neurones (1986)
    Springer
    The journal of membrane biology 93 (1986), S. 75-83 
    Details
    ISSN: 1432-1424
    Schlagwort(e): single channel ; chloride ; selectivity ; conductance state ; Aplysia neurones
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Summary Single-channel recordings from outside-out patches ofAplysia neurones in K-free solutions revealed the presence in most membrane patches of ionic channels showing surprising selectivity properties, as deduced from reversal potential measurements. After complete substitution of external NaCl by mannitol (in the presence of internal CsCl), these channels are more permeable to Cl than to Cs, but are also slightly permeable to Cs:P Cl/P Cs=4. Furthermore, in the presence of external NaCl, their ability to discriminate cations from anions seems lower than in external mannitol. Substitutions of external Cl by various anions showed that the channels are more permeable to NO3 than to Cl, and that they are appreciably permeable to isethionate, SO4 and methanesulfonate. Their elementary conductance is about 100 pS in 600mm symmetrical Cl. However, different conductance states (usually 2 or 3) can often be detected in the same membrane patch. By using voltage ramps, we established theI–V curves corresponding to each of these states and found small but significant differences between the reversal potentials of each state.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01871020
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  • 8
    Digitale Medien
    Digitale Medien
    Auxin inhibition of acid-and fusicoccin-induced elongation in lentil roots (1977)
    Springer
    Planta 136 (1977), S. 97-102 
    Details
    ISSN: 1432-2048
    Schlagwort(e): Acid growth ; Auxin ; Ethylene ; Fusicoccin ; Growth inhibition ; Lens ; Root growth
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Both acid pH (4.0) and fusicoccin (FC) strongly stimulate root elongation in intact lentil (Lens culinaris Med.) seedlings. FC-induced elongation is apparently mediated by FC-enhanced H+ secretion since the toxin induces massive secretion of H+ in these roots after a latent period of less than 5 min. Auxin (indole-3-acetic acid) strongly inhibits elongation in control roots as well as acid-induced and FC-induced root elongation. Treatment of apical root segments with auxin causes only a slight apparent uptake of H+ and has no inhibitory effect on FC-induced H+ secretion, whether the hormone is given before or after the toxin. Auxin induces ethylene production in excised roots of lentil but the latent period is at least 30 min while inhibition of root elongation by IAA is maximal within 30 min. It is concluded that the inhibitory action of auxin on acid-and fusicoccin-induced root elongation is a direct effect, independent of auxin-induced ethylene production or auxin-mediated modification of cell-wall pH.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00396184
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  • 9
    Digitale Medien
    Digitale Medien
    Binding of 45Ca2+ to particulate fractions of coleoptile tissue (1976)
    Springer
    Planta 130 (1976), S. 295-301 
    Details
    ISSN: 1432-2048
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Using recently developed techniques, we have investigated the binding of 45Ca2+ to membrane preparations from corn (Zea mays L) and oat (Avena sativa L) coleoptile tissue. Scatchard plot analysis reveals at least two Ca2+-binding sites in each tissue, a high affinity binding site (K m=7.7×10-7 M, n=6.9×10-10 mol·0.5 g f.w.-1 in corn, K m=4.93×10-6 M, n=2.29×10-9 mol·0.5 g f.w.-1 in Avena) and a low affinity binding site (K m=9.01×10-5 M, n=5.4×10-8 mol·0.5 g f.w.-1 in corn; K m=1.03×10-4 M, n=3.40×10-8 mol·0.5 g f.w.-1 in Avena). There is also some evidence of a third, lower affinity binding site in each tissue, especially corn. More detailed studies with corn coleoptile homogenates show that they contain a potent dialyzable inhibitor of Ca2+ binding. Monovalent cations were observed to be ineffective as inhibitors of Ca2+ binding in corn. However, of six divalent cations tested, all were capable of strong inhibition of Ca2+-binding and there appeared to be a relationship between size of the atomic radius of the ion and potency as an inhibitor of calcium binding.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00387836
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  • 10
    Digitale Medien
    Digitale Medien
    Plasma fluorescein and fluorescein glucuronide in patients with selected eye diseases (1989)
    Springer
    Graefe's archive for clinical and experimental ophthalmology 227 (1989), S. 114-117 
    Details
    ISSN: 1435-702X
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Systemically administered fluorescein (F) is rapidly transformed to the fluorescent metabolite fluorescein glucuronide (FG). Little is known about how diseases can influence the synthesis or disposition of FG. We studied F and FG in the plasma ultrafiltrate of 75 people who were normal or had diabetes, retinitis pigmentosa, or idiopathic rhegmatogenous retinal detachment. F and FG were determined by high-performance liquid chromatography. The concentration of FG was comparable to F 1 h after an intravenous injection of F, both in normal subjects and in patients with retinitis pigmentosa, which suggests that FG may not be an important contributor to the vitreous fluorescence at that time. At later times FG substantially exceeded F. The concentration of FG was significantly higher in diabetics than in the other groups 14 h after an oral dose of F. Accordingly, the possible effect of disease on plasma dye concentrations should be considered in studies measuring F by fluorescence hours after systemic F administration, since this could influence the intraocular fluorescence irrespective of any alteration in ocular function.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02169781
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