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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of cutaneous pathology 21 (1994), S. 0 
    ISSN: 1600-0560
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Normal skin was cryoprotected by submerging it in a mixture of 30% dimethylformamide (DMF) in PBS or RPMI. Subsequently it was frozen in liquid propane gas. Cryosubstitution was carried out at −90°C by using methanol to which uranyl acetate or osmium tetroxide were added. The tissue was embedded in either Lowicryl K4M at −40°C or in Epon at +60°C. The- tissue was evaluated by its overall preservation of ultras-tructural details and by its labeling intensity alter incubation with either anti-clesmoglein or anti-type VII collagen monoclonal antibodies. The mixture of DMF and PBS caused an electron-dense precipitate within the cell. The overall morphology was better in Epon-embedded material than in K4M-embedclecl material. However, the labeling was best in K4M material. Regardless of whether the tissue was embedded in Epon or K4M, the addition of osmium tetroxide markedly reduced the degree of labeling.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of cutaneous pathology 21 (1994), S. 0 
    ISSN: 1600-0560
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: An-11-day old girl was seen with brownish nodular lesions scattered over (he body with emphasis on the face and seal). Several lesions had started lo involute. Tissue was studied by histopathology, immunohistopathology, routine electron microscopy, and immuno-electron microscopy using cryosubstitution and embedding in K4M. Immunohistopathology revealed that the cells of the dermal infiltrate were Langerhans cells. They expressed Leu 6 and HLA-DR. On routine electron microscopy no Birbeck granules were (bund in the dermal cells. Birbeck granules in epidermal Langerhans cells were deformed and often situated next to laminated dense bodies. The latter expressed Leu 6 and HLA-DR. on immuno-electron microscopy. It was concluded that congenital self-healing reticulohistiocytosis is a benign Langerhans cell disease in which Birbeck granules are transformed to laminated dense bodies and possibly degraded by lysosomal enzymes.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 38 (1991), S. 0 
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: In 1985 and 1986 ichthyoplankton composition was studied during seven sampling series at 37 stations along the tidal Elbe and its tributary rivers between Hamburg harbour and the North Sea. The highest larval density was observed in May and April. No, or very few, larvae occurred from late summer to early spring. Smelt (59% on average of all samples) and twaite shad (20%) dominate in the freshwater region. Herring/sprat (17%) dominate in the estuary. All major spawning grounds are located along the southern shore. Smelt spawn further upstream 1 month earlier than twaite shad. Three percent of all larvae found were flounder. Most of them occurred upstream from the estuary in the R. Este and in other tributary rivers. It is not clear whether flounders are able to spawn in freshwater. Attention is drawn to the fact that an unknown but probably very high percentage of the ichthyoplankton is entrained by local power plants.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of applied ichthyology 7 (1991), S. 0 
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: For three selected weeks in July, August, and October 1985, catches from anchor net vessels at ten stations along the tidal Elbe River (northern Germany) were analyzed and related to oxygen conditions in the river. The oxygen concentrations decreased from an average of 6.6 ml/L in the estuary to an average of 1.4 mg/L upstream. Fish were found to be concentrated downstream from the low-oxygen zone and at the entrance to a shallow side arm of the main stream. Highest catches of eel were made where oxygen levels were in the range of 1.2–3 mg/L, highest catches of flounder at 3–4 mg/L, and highest catches of juvenile and adult smelt in areas with more than 5 mgO2/L. Such a situation enables fishermen to make extraordinarily high catches. It is concluded that despite high levels of toxicants, the tidal Elbe still offers living conditions adequate for the mass of fish species as long as there is a sufficient supply of oxygen.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford [u.a.] : International Union of Crystallography (IUCr)
    Acta crystallographica 49 (1993), S. 944-946 
    ISSN: 1600-5759
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    The European physical journal 212 (1968), S. 107-121 
    ISSN: 1434-601X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Using recoilless gamma resonance absorption of the 23.8 keV transition of Sn119 we have measured the isomer shift as a function of hydrostatic pressure up to 100 kbar. The tin isotope was introduced into the substances SnMg2, metallic tin, Pd(12 wgt.% Sn), Sn (SO4)2·2H2O, and SnAu. Except for SnMg2, all compounds exhibit a linear relation between isomer shift and pressure, which reflects a decrease of ¦ ψ(0)¦2, the probability density of the electrons at the nuclear site, with increasing pressure. This decrease is interpreted by a volume-dependent screening of the 5s electrons by the 5p electrons. The non-linear dependence of the isomer shift upon pressure in the case of SnMg2 is interpreted in terms of the superposition of two counteracting effects, with band repopulation and electronic shielding effects prevailing in the low and high pressure branches, respectively. Measurements of the pressure dependence of both the electrical resistivity and the lattice structure of SnMg2 lead to a picture, which resulted in a calculation of the pressure dependence of the isomer shift, which is in agreement with the experimental observations.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Journal of molecular medicine 69 (1991), S. 413-415 
    ISSN: 1432-1440
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 190 (1938), S. 216-217 
    ISSN: 1432-1912
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 192 (1939), S. 708-714 
    ISSN: 1432-1912
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Zusammenfassung Sinn der angestellten Versuche sollte ein Vergleich beider Präparate bezüglich ihrer Verträglichkeit bzw. Toxizität sein. Die Prüfung an Mäusen, Ratten und Katzen zeigt deutlich, da\ bei obiger Fragestellung das p-Sulfanilamido-acetophenon dem Sulfanilamid wesentlich überlegen ist. Ich bin mir bewußt, daß die verabreichten Mengen außerhalb der therapeutischen Dosierung liegen und nur aus der Versuchsanordnung heraus berechtigt sind.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Archives of dermatological research 284 (1992), S. 242-245 
    ISSN: 1432-069X
    Keywords: Immunogold ; Electron microscopy ; Human skin ; Actin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Normal human skin was embedded in Lowicryl K4M. Actin microfilaments were localized by applyinga postembedding immunogold technique using the monoclonal anti-actin antibody HHF35. Actin microfilaments are part of the cytoskeleton in muscle and nonmuscle cells. Together with myosin they produce contraction. The antibody labelled myofilaments in smooth muscle arrector pili cells, myoepithelial cells and pericytes. In sweat gland cells the microvilli system, a zone beneath the cytoplasma membrane correponding to the adhesion belt region, and apocrine decapitation formations showed labelling.
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