GLORIA

GEOMAR Library Ocean Research Information Access

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • 2020-2023  (14)
  • 2010-2014  (84)
Document type
Keywords
Publisher
Years
Year
  • 1
    facet.materialart.
    Unknown
    General responses of Thoracosphaera heimii grown under a range of pCO2 (2013)
    PANGAEA
    In:  Supplement to: Van de Waal, Dedmer B; John, Uwe; Ziveri, Patrizia; Reichart, Gert-Jan; Hoins, Mirja; Sluijs, Appy; Rost, Björn (2013): Ocean Acidification Reduces Growth and Calcification in a Marine Dinoflagellate. PLoS ONE, 8(6), e65987, https://doi.org/10.1371/journal.pone.0065987
    Details
    Publication Date: 2023-08-03
    Description: Ocean acidification is considered a major threat to marine ecosystems and may particularly affect calcifying organisms such as corals, foraminifera and coccolithophores. Here we investigate the impact of elevated pCO2 and lowered pH on growth and calcification in the common calcareous dinoflagellate Thoracosphaera heimii. We observe a substantial reduction in growth rate, calcification and cyst stability of T. heimii under elevated pCO2. Furthermore, transcriptomic analyses reveal CO2 sensitive regulation of many genes, particularly those being associated to inorganic carbon acquisition and calcification. Stable carbon isotope fractionation for organic carbon production increased with increasing pCO2 whereas it decreased for calcification, which suggests interdependence between both processes. We also found a strong effect of pCO2 on the stable oxygen isotopic composition of calcite, in line with earlier observations concerning another T. heimii strain. The observed changes in stable oxygen and carbon isotope composition of T. heimii cysts may provide an ideal tool for reconstructing past seawater carbonate chemistry, and ultimately past pCO2. Although the function of calcification in T. heimii remains unresolved, this trait likely plays an important role in the ecological and evolutionary success of this species. Acting on calcification as well as growth, ocean acidification may therefore impose a great threat for T. heimii.
    Keywords: Calculated; Carbon, inorganic, particulate, per cell; Carbon, organic, particulate, per cell; Carbon dioxide; Cysts; Fractionation of calcite; Fractionation of organic carbon; Growth rate; Mediterranean Sea Acidification in a Changing Climate; MedSeA; Ratio; Treatment; δ18O, calcite; δ18O, dissolved inorganic carbon
    Type: Dataset
    Format: text/tab-separated-values, 144 data points
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    DATA PANGAEA
  • 2
    facet.materialart.
    Unknown
    (Table 1) Biological and chemical characteristics of surface water stations during Nathaniel B. Palmer cruise NBP06-08 to the Ross Sea (2010)
    PANGAEA
    In:  Supplement to: Tortell, Philippe Daniel; Trimborn, Scarlett; Li, Y; Rost, Björn; Payne, Christopher D (2010): Inorganic carbon utilization by Ross Sea phytoplankton across natural and experimental CO2 gradients. Journal of Phycology, 46(3), 433-443, https://doi.org/10.1111/j.1529-8817.2010.00839.x
    Details
    Publication Date: 2023-12-12
    Description: We present results from a field study of inorganic carbon (C) acquisition by Ross Sea phytoplankton during Phaeocystis-dominated early season blooms. Isotope disequilibrium experiments revealed that HCO3- was the primary inorganic C source for photosynthesis in all phytoplankton assemblages. From these experiments, we also derived relative enhancement factors for HCO3-/CO2 interconversion as a measure of extracellular carbonic anhydrase activity (eCA). The enhancement factors ranged from 1.0 (no apparent eCA activity) to 6.4, with an overall mean of 2.9. Additional eCA measurements, made using membrane inlet mass spectrometry (MIMS), yielded activities ranging from 2.4 to 6.9 U/[mg chl a] (mean 4.1). Measurements of short-term C-fixation parameters revealed saturation kinetics with respect to external inorganic carbon, with a mean half-saturation constant for inorganic carbon uptake (K1/2) of ~380 mM. Comparison of our early springtime results with published data from late-season Ross Sea assemblages showed that neither HCO3- utilization nor eCA activity was significantly correlated to ambient CO2 levels or phytoplankton taxonomic composition. We did, however, observe a strong negative relationship between surface water pCO2 and short-term 14C-fixation rates for the early season survey. Direct incubation experiments showed no statistically significant effects of pCO2 (10 to 80 Pa) on relative HCO3- utilization or eCA activity. Our results provide insight into the seasonal regulation of C uptake by Ross Sea phytoplankton across a range of pCO2 and phytoplankton taxonomic composition.
    Keywords: Carbon dioxide, partial pressure; Chlorophyll a; CORSACS II; Depth, bottom/max; Depth, top/min; DEPTH, water; Dominant species of live fauna; Event label; Latitude of event; Longitude of event; Nathaniel B. Palmer; NBP0608; NBP0608_SW14; NBP0608_SW20; NBP0608_SW21; NBP0608_SW23; NBP0608_SW28; NBP0608_SW32; NBP0608_SW35; NBP0608_SW38; NBP0608_SW41; NBP0608_SW42; NBP0608_SW43; NBP0608_SW44; NBP0608_SW45; NBP0608_SW46; NBP0608_SW47; NBP0608_SW48; Nitrate; Priority Programme 1158 Antarctic Research with Comparable Investigations in Arctic Sea Ice Areas; Ross Sea; Silicon dioxide; Southern Ocean; SPP1158; Surface water sample; SWS
    Type: Dataset
    Format: text/tab-separated-values, 108 data points
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    DATA PANGAEA
  • 3
    facet.materialart.
    Unknown
    Seawater carbonate chemistry and combined physiological effects of CO2 and light on the N2-fixing cyanobacterium Trichodesmium IMS101 during experiments, 2010 (2010)
    PANGAEA
    In:  Supplement to: Kranz, Sven A; Levitan, Orly; Richter, Klaus-Uwe; Prasil, O; Beran-Frank, Ilana; Rost, Björn (2010): Combined effects of CO2 and light on the N2-fixing cyanobacterium Trichodesmium IMS101: Physiological responses. Plant Physiology, 154, 334-345, https://doi.org/10.1104/pp.110.159145
    Details
    Publication Date: 2024-03-15
    Description: Recent studies on the diazotrophic cyanobacterium Trichodesmium erythraeum(IMS101) showed that increasing CO2 partial pressure (pCO2) enhances N2 fixation and growth. Significant uncertainties remain as to the degree of the sensitivity to pCO2, its modification by other environmental factors, and underlying processes causing these responses. To address these questions, we examined the responses ofTrichodesmium IMS101 grown under a matrix of low and high levels of pCO2 (150 and 900 µatm) and irradiance (50 and 200 µmol photons m-2 s-1). Growth rates as well as cellular carbon and nitrogen contents increased with increasing pCO2 and light levels in the cultures. The pCO2-dependent stimulation in organic carbon and nitrogen production was highest under low light. High pCO2 stimulated rates of N2fixation and prolonged the duration, while high light affected maximum rates only. Gross photosynthesis increased with light but did not change with pCO2. HCO3- was identified as the predominant carbon source taken up in all treatments. Inorganic carbon uptake increased with light, but only gross CO2 uptake was enhanced under high pCO2. A comparison between carbon fluxes in vivo and those derived from 13C fractionation indicates high internal carbon cycling, especially in the low-pCO2treatment under high light. Light-dependent oxygen uptake was only detected underlow pCO2 combined with high light or when low-light-acclimated cells were exposed to high light, indicating that the Mehler reaction functions also as a photoprotective mechanism in Trichodesmium. Our data confirm the pronounced pCO2 effect on N2fixation and growth in Trichodesmium and further show a strong modulation of these effects by light intensity. We attribute these responses to changes in the allocation of photosynthetic energy between carbon acquisition and the assimilation of carbon and nitrogen under elevated pCO2. These findings are supported by a complementarystudy looking at photosynthetic fluorescence parameters of photosystem II, photosynthetic unit stoichiometry (photosystem I:photosystem II), and pool sizes of key proteins in carbon and nitrogen acquisition.
    Keywords: Alkalinity, Gran titration (Gran, 1950); Alkalinity, total; Alkalinity, total, standard deviation; Aragonite saturation state; Bacteria; Bicarbonate ion; Bicarbonate uptake in chlorophyll, standard deviation; Bicarbonate uptake rate, per chlorophyll a; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (〈20 L); Calcite saturation state; Calculated; Calculated, see reference(s); Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbon, organic, particulate, per cell; Carbon/Nitrogen ratio; Carbon/Nitrogen ratio, standard deviation; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Carbon dioxide, standard deviation; Carbon dioxide uptake, per chlorophyll, standard deviation; Carbon dioxide uptake rate, per chlorophyll a; Chlorophyll a, standard deviation; Chlorophyll a per cell; Comment; Cyanobacteria; Determined by acetylene reduction assay using a gas chromatograph; EPOCA; EUR-OCEANS; European network of excellence for Ocean Ecosystems Analysis; European Project on Ocean Acidification; Experimental treatment; Fixation of carbon in chlorophyll; Fixation of carbon in chlorophyll, standard deviation; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gross oxygen evolution, per chlorophyll a; Gross oxygen evolution, standard deviation; Growth/Morphology; Growth rate; Growth rate, standard deviation; Laboratory experiment; Laboratory strains; Light; Mass spectrometer ANCA-SL 20-20 Europa Scientific; Nitrogen fixation rate, standard deviation; Nitrogen fixation rate per chlorophyll a; Not applicable; OA-ICC; Ocean Acidification International Coordination Centre; Other metabolic rates; Oxygen consumption, standard deviation; Oxygen consumption per chlorophyll a; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Particulate organic carbon, production, standard deviation; Particulate organic carbon content per cell, standard deviation; Particulate organic carbon production per cell; Particulate organic nitrogen per cell; Particulate organic nitrogen per cell, standard deviation; Particulate organic nitrogen production, standard deviation; Particulate organic phosphorus per cell; Particulate organic phosphorus per cell, standard deviation; Pelagos; pH; pH, standard deviation; pH meter (Metrohm electrodes); Phytoplankton; Primary production/Photosynthesis; Production of particulate organic nitrogen; Radiation, photosynthetically active; Respiration; Salinity; see reference(s); Single species; Temperature, water; Time in hours; Trichodesmium sp.
    Type: Dataset
    Format: text/tab-separated-values, 1788 data points
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    DATA PANGAEA
  • 4
    facet.materialart.
    Unknown
    Seawater carbonate chemistry and combined mechanistic effects of CO2 and light on the N2-fixing cyanobacterium Trichodesmium IMS101, 2010 (2010)
    PANGAEA
    In:  Supplement to: Levitan, Orly; Kranz, Sven A; Spungin, D; Prasil, O; Rost, Björn; Beran-Frank, Ilana (2010): Combined effects of CO2 and light on the N2-fixing cyanobacterium Trichodesmium IMS101: A mechanistic view. Plant Physiology, 154, 346-356, https://doi.org/10.1104/pp.110.159285
    Details
    Publication Date: 2024-03-15
    Description: The marine diazotrophic cyanobacterium Trichodesmium responds to elevated atmospheric CO2 partial pressure (pCO2) with higher N2 fixation and growth rates. To unveil the underlying mechanisms, we examined the combined influence of pCO2(150 and 900 µatm) and light (50 and 200 µmol photons m-2 s-1) on TrichodesmiumIMS101. We expand on a complementary study that demonstrated that while elevated pCO2 enhanced N2 fixation and growth, oxygen evolution and carbon fixation increased mainly as a response to high light. Here, we investigated changes in the photosynthetic fluorescence parameters of photosystem II, in ratios of the photosynthetic units (photosystem I:photosystem II), and in the pool sizes of key proteins involved in the fixation of carbon and nitrogen as well as their subsequent assimilation. We show that the combined elevation in pCO2 and light controlled the operation of the CO2-concentrating mechanism and enhanced protein activity without increasing their pool size. Moreover, elevated pCO2 and high light decreased the amounts of several key proteins (NifH, PsbA, and PsaC), while amounts of AtpB and RbcL did not significantly change. Reduced investment in protein biosynthesis, without notably changing photosynthetic fluxes, could free up energy that can be reallocated to increase N2 fixation and growth at elevated pCO2 and light. We suggest that changes in the redox state of the photosynthetic electron transportchain and posttranslational regulation of key proteins mediate the high flexibility in resources and energy allocation in Trichodesmium. This strategy should enableTrichodesmium to flourish in future surface oceans characterized by elevated pCO2, higher temperatures, and high light.
    Keywords: Alkalinity, Gran titration (Gran, 1950); Alkalinity, total; Aragonite saturation state; Bacteria; Bicarbonate ion; Bottles or small containers/Aquaria (〈20 L); Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; CF1 subunit of ATP synthase protein; CF1 subunit of ATP synthase protein, standard deviation; Comment; Cyanobacteria; Effective absorbance cross-section of photosystem II; Effective absorbance cross-section of photosystem II, standard deviation; Electron transport rate of photosystem II, per cell; Electron transport rate of photosystem II, standard deviation; EPOCA; EUR-OCEANS; European network of excellence for Ocean Ecosystems Analysis; European Project on Ocean Acidification; Experimental treatment; Fluorescence, intrinsic; Fluorescence, intrinsic, standard deviation; Fluorescence, maximum; Fluorescence, maximum, standard deviation; Fluorescence, variable; Fluorescence, variable, standard deviation; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); GlnA subunit of Gln synthetase; GlnA subunit of Gln synthetase, standard deviation; Iron protein of nitrogenase; Iron protein of nitrogenase, standard deviation; Laboratory experiment; Laboratory strains; Light; Maximum photochemical quantum yield of photosystem II; Maximum photochemical quantum yield of photosystem II, standard deviation; Not applicable; OA-ICC; Ocean Acidification International Coordination Centre; Open photosystem II reaction centers; Open photosystem II reaction centers, standard deviation; Other metabolic rates; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH; Photosynthetic protein, PsbA, standard deviation; Photosynthetic protein, PsbC; Photosynthetic protein, PsbC, standard deviation; Photosynthetic protein PsbA; Photosynthetic protein Rubisco; Photosynthetic protein Rubisco, standard deviation; Phytoplankton; Primary production/Photosynthesis; Radiation, photosynthetically active; Salinity; see reference(s); Single species; Temperature, water; Time in hours; Trichodesmium sp.
    Type: Dataset
    Format: text/tab-separated-values, 608 data points
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    DATA PANGAEA
  • 5
    facet.materialart.
    Unknown
    Seawater carbonate chemistry, chlorophyll a and phosphate during experiments with Trichodesmium erythraeum IMS101 (CCMP1985), 2010 (2010)
    PANGAEA
    In:  Supplement to: Kranz, Sven A; Wolf-Gladrow, Dieter A; Nehrke, Gernot; Langer, Gerald; Rost, Björn (2010): Calcium carbonate precipitation induced by the growth of the marine cyanobacteria Trichodesmium. Limnology and Oceanography, 55(6), 2563-2569, https://doi.org/10.4319/lo.2010.55.6.2563
    Details
    Publication Date: 2024-03-15
    Description: In this laboratory study, we monitored the buildup of biomass and concomitant shift in seawater carbonate chemistry over the course of a Trichodesmium bloom under different phosphorus (P) availability. During exponential growth, dissolved inorganic carbon (DIC) decreased, while pH increased until maximum cell densities were reached. Once P became depleted, DIC decreased even further and total alkalinity (TA) dropped, accompanied by precipitation of aragonite. Under P-replete conditions, DIC increased and TA remained constant in the postbloom phase. A diffusion-reaction model was employed to estimate changes in carbonate chemistry of the diffusive boundary layer. This study demonstrates that Trichodesmium can induce precipitation of aragonite from seawater and further provides possible explanations about underlying mechanisms.
    Keywords: Alkalinity, Gran titration (Gran, 1950); Alkalinity, total; Aragonite saturation state; Auto-analyzer, Technicon Traacs 800; Bacteria; Bicarbonate ion; BIOACID; Biological Impacts of Ocean Acidification; Bottles or small containers/Aquaria (〈20 L); Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Chlorophyll a; Comment; Conductivity meter (WTW, Weilheim, Gemany); Cyanobacteria; EPOCA; EUR-OCEANS; European network of excellence for Ocean Ecosystems Analysis; European Project on Ocean Acidification; Experimental treatment; Experiment day; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Laboratory experiment; Laboratory strains; Light:Dark cycle; Measured; Not applicable; OA-ICC; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH; Phosphate; Phytoplankton; Radiation, photosynthetically active; Salinity; see reference(s); Single species; Species; Temperature, water; Trichodesmium erythraeum
    Type: Dataset
    Format: text/tab-separated-values, 2017 data points
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    DATA PANGAEA
  • 6
    facet.materialart.
    Unknown
    Combined effects of different CO2 levels and N sources on the diazotrophic cyanobacterium Trichodesmium (2014)
    PANGAEA
    In:  Supplement to: Eichner, Meri; Kranz, Sven A; Rost, Björn (2014): Combined effects of different CO2 levels and N sources on the diazotrophic cyanobacterium Trichodesmium. Physiologia Plantarum, 152(2), 316-330, https://doi.org/10.1111/ppl.12172
    Details
    Publication Date: 2024-03-15
    Description: To predict effects of climate change and possible feedbacks, it is crucial to understand the mechanisms behind CO2 responses of biogeochemically relevant phytoplankton species. Previous experiments on the abundant N2 fixers Trichodesmium demonstrated strong CO2 responses, which were attributed to an energy reallocation between its carbon (C) and nitrogen (N) acquisition. Pursuing this hypothesis, we manipulated the cellular energy budget by growing Trichodesmium erythraeum IMS101 under different CO2 partial pressure (pCO2) levels (180, 380, 980 and 1400?µatm) and N sources (N2 and NO3-). Subsequently, biomass production and the main energy-generating processes (photosynthesis and respiration) and energy-consuming processes (N2 fixation and C acquisition) were measured. While oxygen fluxes and chlorophyll fluorescence indicated that energy generation and its diurnal cycle was neither affected by pCO2 nor N source, cells differed in production rates and composition. Elevated pCO2 increased N2 fixation and organic C and N contents. The degree of stimulation was higher for nitrogenase activity than for cell contents, indicating a pCO2 effect on the transfer efficiency from N2 to biomass. pCO2-dependent changes in the diurnal cycle of N2 fixation correlated well with C affinities, confirming the interactions between N and C acquisition. Regarding effects of the N source, production rates were enhanced in NO3-grown cells, which we attribute to the higher N retention and lower ATP demand compared with N2 fixation. pCO2 effects on C affinity were less pronounced in NO3- users than N2 fixers. Our study illustrates the necessity to understand energy budgets and fluxes under different environmental conditions for explaining indirect effects of rising pCO2.
    Keywords: Alkalinity, total; Alkalinity, total, standard deviation; Aragonite saturation state; Bacteria; Bicarbonate ion; Bottles or small containers/Aquaria (〈20 L); Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, half saturation concentration; Carbon, inorganic, dissolved, half saturation concentration, standard deviation; Carbon, inorganic, dissolved, standard deviation; Carbon, organic, particulate, standard deviation; Carbon, organic, particulate/Nitrogen, organic, particulate ratio; Carbon, organic, particulate/Nitrogen, organic, particulate ratio, standard deviation; Carbon, organic, particulate per chlorophyll a; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Coulometric titration; Cyanobacteria; Effective absorbance cross-section of photosystem II; Effective absorbance cross-section of photosystem II, standard deviation; Figure; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Growth/Morphology; Growth rate; Growth rate, standard deviation; Identification; Irradiance; Laboratory experiment; Laboratory strains; Light:Dark cycle; Macro-nutrients; Maximum photochemical quantum yield of photosystem II; Maximum photochemical quantum yield of photosystem II, standard deviation; Net oxygen evolution, per chlorophyll a; Net oxygen evolution, per chlorophyll a, standard deviation; Nitrogen, organic, particulate, standard deviation; Nitrogen, organic, particulate per chlorophyll a; Nitrogen fixation rate per chlorophyll a; Not applicable; OA-ICC; Ocean Acidification International Coordination Centre; Other metabolic rates; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Particulate organic carbon, production, standard deviation; Particulate organic carbon production, per chlorophyll a; Particulate organic nitrogen production, per chlorophyll a; Particulate organic nitrogen production, standard deviation; Pelagos; pH; pH, standard deviation; Phytoplankton; Potentiometric; Potentiometric titration; Primary production/Photosynthesis; Re-oxidation time of the Qa acceptor; Re-oxidation time of the Qa acceptor, standard deviation; Respiration; Salinity; Single species; Species; Temperature, water; Time in hours; Time point, descriptive; Treatment; Trichodesmium erythraeum
    Type: Dataset
    Format: text/tab-separated-values, 1523 data points
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    DATA PANGAEA
  • 7
    facet.materialart.
    Unknown
    Ocean Acidification Affects Redox-Balance and Ion-Homeostasis in the Life-Cycle Stages of Emiliania huxleyi (2012)
    PANGAEA
    In:  Supplement to: Rokitta, Sebastian D; John, Uwe; Rost, Björn (2012): Ocean Acidification Affects Redox-Balance and Ion-Homeostasis in the Life-Cycle Stages of Emiliania huxleyi. PLoS ONE, 7(12), e52212, https://doi.org/10.1371/journal.pone.0052212
    Details
    Publication Date: 2024-03-15
    Description: Ocean Acidification (OA) has been shown to affect photosynthesis and calcification in the coccolithophore Emiliania huxleyi, a cosmopolitan calcifier that significantly contributes to the regulation of the biological carbon pumps. Its non-calcifying, haploid life-cycle stage was found to be relatively unaffected by OA with respect to biomass production. Deeper insights into physiological key processes and their dependence on environmental factors are lacking, but are required to understand and possibly estimate the dynamics of carbon cycling in present and future oceans. Therefore, calcifying diploid and non-calcifying haploid cells were acclimated to present and future CO2 partial pressures (pCO2; 38.5 Pa vs. 101.3 Pa CO2) under low and high light (50 vs. 300 µmol photons/m**2 /s). Comparative microarray-based transcriptome profiling was used to screen for the underlying cellular processes and allowed to follow up interpretations derived from physiological data. In the diplont, the observed increases in biomass production under OA are likely caused by stimulated production of glycoconjugates and lipids. The observed lowered calcification under OA can be attributed to impaired signal-transduction and ion-transport. The haplont utilizes distinct genes and metabolic pathways, reflecting the stage-specific usage of certain portions of the genome. With respect to functionality and energy-dependence, however, the transcriptomic OA-responses resemble those of the diplont. In both life-cycle stages, OA affects the cellular redox-state as a master regulator and thereby causes a metabolic shift from oxidative towards reductive pathways, which involves a reconstellation of carbon flux networks within and across compartments. Whereas signal transduction and ion-homeostasis appear equally OA-sensitive under both light intensities, the effects on carbon metabolism and light physiology are clearly modulated by light availability. These interactive effects can be attributed to the influence of OA and light on the redox equilibria of NAD and NADP, which function as major sensors for energization and stress. This generic mode of action of OA may therefore provoke similar cell-physiological responses in other protists.
    Keywords: Alkalinity, total; Alkalinity, total, standard deviation; Aragonite saturation state; Bicarbonate ion; Bicarbonate ion, standard deviation; Bottles or small containers/Aquaria (〈20 L); Calcite saturation state; Calcite saturation state, standard deviation; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Carbonate system computation flag; Carbon dioxide; Category; Chromista; Emiliania huxleyi; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gene abundance; Gene expression (incl. proteomics); Group; Haptophyta; Irradiance; Laboratory experiment; Laboratory strains; Light; North Atlantic; OA-ICC; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH; pH, standard deviation; Phytoplankton; Potentiometric; Potentiometric titration; Salinity; Single species; Species; Strain; Temperature, water
    Type: Dataset
    Format: text/tab-separated-values, 696 data points
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    DATA PANGAEA
  • 8
    facet.materialart.
    Unknown
    Photophysiological responses of Southern Ocean phytoplankton to changes in CO2 concentrations: Short-term versus acclimation effects (2014)
    PANGAEA
    In:  Supplement to: Trimborn, Scarlett; Thoms, Silke; Petrou, Katherina; Kranz, Sven A; Rost, Björn (2014): Photophysiological responses of Southern Ocean phytoplankton to changes in CO2 concentrations: Short-term versus acclimation effects. Journal of Experimental Marine Biology and Ecology, 451, 44-54, https://doi.org/10.1016/j.jembe.2013.11.001
    Details
    Publication Date: 2024-03-15
    Description: The present study examines how different pCO2 acclimations affect the CO2- and light-dependence of photophysiological processes and O2 fluxes in four Southern Ocean (SO) key phytoplankton species. We grew Chaetoceros debilis (Cleve), Pseudo-nitzschia subcurvata (Hasle), Fragilariopsis kerguelensis (O'Meara) and Phaeocystis antarctica (Karsten) under low (160 µatm) and high (1000 ?atm) pCO2. The CO2- and light-dependence of fluorescence parameters of photosystem II (PSII) were determined by means of a fluorescence induction relaxation system (FIRe). In all tested species, nonphotochemical quenching (NPQ) is the primary photoprotection strategy in response to short-term exposure to high light or low CO2 concentrations. In C. debilis and P. subcurvata, PSII connectivity (p) and functional absorption cross-sections of PSII in ambient light (sigma PSII') also contributed to photoprotection while changes in re-oxidation times of Qa acceptor (tQa) were more significant in F. kerguelensis. The latter was also the only species being responsive to high acclimation pCO2, as these cells had enhanced relative electron transport rates (rETRs) and sigma PSII' while tQa and p were reduced under short-term exposure to high irradiance. Low CO2-acclimated cells of F. kerguelensis and all pCO2 acclimations of C. debilis and P. subcurvata showed dynamic photoinhibition with increasing irradiance. To test for the role and presence of the Mehler reaction in C. debilis and P. subcurvata, the light-dependence of O2 fluxes was estimated using membrane inlet mass spectrometry (MIMS). Our results show that the Mehler reaction is absent in both species under the tested conditions. We also observed that dark respiration was strongly reduced under high pCO2 in C. debilis while it remained unaltered in P. subcurvata. Our study revealed species-specific differences in the photophysiological responses to pCO2, both on the acclimation as well as the short-term level.
    Keywords: Alkalinity, total; Alkalinity, total, standard deviation; Antarctic; Aragonite saturation state; Bicarbonate ion; Bottles or small containers/Aquaria (〈20 L); Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Carbon dioxide, standard deviation; Chaetoceros debilis; Chromista; Connectivity between photosystem II; Connectivity between photosystem II, standard deviation; Effective absorbance cross-section of photosystem II; Effective quantum yield; Electron transport rate, relative; Figure; Fragilariopsis kerguelensis; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gross oxygen evolution, per chlorophyll a; Gross oxygen evolution, standard deviation; Haptophyta; Irradiance; Laboratory experiment; Laboratory strains; Net oxygen evolution, per chlorophyll a; Net oxygen evolution, per chlorophyll a, standard deviation; Non photochemical quenching; OA-ICC; Ocean Acidification International Coordination Centre; Ochrophyta; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH; pH, standard deviation; Phaeocystis antarctica; Phytoplankton; Potentiometric; Potentiometric titration; Primary production/Photosynthesis; Priority Programme 1158 Antarctic Research with Comparable Investigations in Arctic Sea Ice Areas; Pseudo-nitzschia subcurvata; Re-oxidation time of the Qa acceptor; Re-oxidation time of the Qa acceptor, standard deviation; Respiration; Respiration rate, oxygen; Respiration rate, oxygen, standard deviation; Salinity; Single species; Species; SPP1158; Table; Temperature, water; Treatment
    Type: Dataset
    Format: text/tab-separated-values, 36257 data points
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    DATA PANGAEA
  • 9
    facet.materialart.
    Unknown
    Seawater carbonate chemistry and biological processes of Emiliania huxleyi (strains RCC1256 and NZEH) during experiments, 2011 (2011)
    PANGAEA
    In:  Supplement to: Hoppe, Clara Jule Marie; Langer, Gerald; Rost, Björn (2011): Emiliania huxleyi shows identical responses to elevated pCO2 in TA and DIC manipulations. Journal of Experimental Marine Biology and Ecology, 406(1-2), 54-62, https://doi.org/10.1016/j.jembe.2011.06.008
    Details
    Publication Date: 2024-03-15
    Description: With respect to their sensitivity to ocean acidification, calcifiers such as the coccolithophore Emiliania huxleyi have received special attention, as the process of calcification seems to be particularly sensitive to changes in the marine carbonate system. For E. huxleyi, apparently conflicting results regarding its sensitivity to ocean acidification have been published (Iglesias-Rodriguez et al., 2008a; Riebesell et al., 2000). As possible causes for discrepancies, intra-specific variability and different effects of CO2 manipulation methods, i.e. the manipulation of total alkalinity (TA) or total dissolved inorganic carbon (DIC), have been discussed. While Langer et al. (2009) demonstrate a high degree of intra-specific variability between strains of E. huxleyi, the question whether different CO2 manipulation methods influence the cellular responses has not been resolved yet. In this study, closed TA as well as open and closed DIC manipulation methods were compared with respect to E. huxleyi's CO2-dependence in growth rate, POC- and PIC-production. The differences in the carbonate chemistry between TA and DIC manipulations were shown not to cause any differences in response patterns, while the latter differed between open and closed DIC manipulation. The two strains investigated showed different sensitivities to acidification of seawater, RCC1256 being more negatively affected in growth rates and PIC production than NZEH.
    Keywords: Alkalinity, Gran titration (Gran, 1950); Alkalinity, total; Aragonite saturation state; Bicarbonate ion; BIOACID; Biological Impacts of Ocean Acidification; Bottles or small containers/Aquaria (〈20 L); Calcification/Dissolution; Calcite saturation state; Calculated; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, particulate, per cell; Carbon, organic, particulate, per cell; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Chromista; Comment; Emiliania huxleyi; EPOCA; EUR-OCEANS; European network of excellence for Ocean Ecosystems Analysis; European Project on Ocean Acidification; Experimental treatment; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Growth/Morphology; Growth rate; Haptophyta; Laboratory experiment; Laboratory strains; Mass spectrometer ANCA-SL 20-20 Europa Scientific; Mediterranean Sea Acidification in a Changing Climate; MedSeA; OA-ICC; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Particulate inorganic carbon/particulate organic carbon ratio; Particulate inorganic carbon production per cell; Particulate organic carbon production per cell; Pelagos; pH; Phytoplankton; Primary production/Photosynthesis; Salinity; Sample ID; Seal QuAAtro SFA Analyzer, Seal Analytical, 800 TM; Single species; South Pacific; Species; Temperature, water
    Type: Dataset
    Format: text/tab-separated-values, 1638 data points
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    DATA PANGAEA
  • 10
    facet.materialart.
    Unknown
    Seawater carbonate chemistry and Emiliania huxleyi mass and size, 2011 (2011)
    PANGAEA
    In:  Supplement to: Beaufort, Luc; Probert, Ian; de Garidel-Thoron, Thibault; Bendif, E M; Ruiz-Pino, Diana; Metzi, N; Goyet, Catherine; Buchet, Noëlle; Coupel, Pierre; Grelaud, Michaël; Rost, Björn; Rickaby, Rosalind E M; De Vargas, Colomban (2011): Sensitivity of coccolithophores to carbonate chemistry and ocean acidification. Nature, 476, 80-83, https://doi.org/10.1038/nature10295
    Details
    Publication Date: 2024-03-15
    Description: About one-third of the carbon dioxide (CO2) released into the atmosphere as a result of human activity has been absorbed by the oceans, where it partitions into the constituent ions of carbonic acid. This leads to ocean acidification, one of the major threats to marine ecosystems and particularly to calcifying organisms such as corals, foraminifera and coccolithophores. Coccolithophores are abundant phytoplankton that are responsible for a large part of modern oceanic carbonate production. Culture experiments investigating the physiological response of coccolithophore calcification to increased CO2 have yielded contradictory results between and even within species. Here we quantified the calcite mass of dominant coccolithophores in the present ocean and over the past forty thousand years, and found a marked pattern of decreasing calcification with increasing partial pressure of CO2 and concomitant decreasing concentrations of CO3. Our analyses revealed that differentially calcified species and morphotypes are distributed in the ocean according to carbonate chemistry. A substantial impact on the marine carbon cycle might be expected upon extrapolation of this correlation to predicted ocean acidification in the future. However, our discovery of a heavily calcified Emiliania huxleyi morphotype in modern waters with low pH highlights the complexity of assemblage-level responses to environmental forcing factors.
    Keywords: Age, dated; Alkalinity, total; Antarctic; Aragonite saturation state; Bicarbonate ion; Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; CTD, Sea-Bird SBE 911plus; Emiliania huxleyi; Emiliania huxleyi, diameter; Emiliania huxleyi, weight; Emiliania huxleyi, weight, standard error; EPOCA; Estimated by measuring brightness in cross-polarized light (birefringence); EUR-OCEANS; European network of excellence for Ocean Ecosystems Analysis; European Project on Ocean Acidification; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Indian Ocean; LATITUDE; LONGITUDE; Measured and/or detected by SYRACO software; North Atlantic; North Pacific; OA-ICC; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH; Phytoplankton; Replicates; Salinity; Sample ID; South Atlantic; South Pacific; Temperature, water; Titration potentiometric
    Type: Dataset
    Format: text/tab-separated-values, 16400 data points
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    DATA PANGAEA
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...