Description: Increasing interest in the acquisition of biotic and abiotic resources from within the deep sea (e.g., fisheries, oil–gas extraction, and mining) urgently imposes the development of novel monitoring technologies, beyond the traditional vessel-assisted, time-consuming, high-cost sampling surveys. The implementation of permanent networks of seabed and water-column-cabled (fixed) and docked mobile platforms is presently enforced, to cooperatively measure biological features and environmental (physicochemical) parameters. Video and acoustic (i.e., optoacoustic) imaging are becoming central approaches for studying benthic fauna (e.g., quantifying species presence, behavior, and trophic interactions) in a remote, continuous, and prolonged fashion. Imaging is also being complemented by in situ environmental-DNA sequencing technologies, allowing the traceability of a wide range of organisms (including prokaryotes) beyond the reach of optoacoustic tools. Here, we describe the different fixed and mobile platforms of those benthic and pelagic monitoring networks, proposing at the same time an innovative roadmap for the automated computing of hierarchical ecological information on deep-sea ecosystems (i.e., from single species’ abundance and life traits to community composition, and overall biodiversity).

Description: The anthropogenic impact of polymetallic nodule harvesting in the Clarion-Clipperton Fracture Zone is expected to strongly affect the benthic ecosystem. To predict the long-term, industrial-scale impact of nodule mining on the deep-sea environment and to improve the reliability of the sediment plume model, information about the specific characteristics of deep-sea particles is needed. Discharge simulations of mining-related fine-grained (median diameter ≈ 20 μm) sediment plumes at concentrations of 35–500 mg L–1 (dry weight) showed a propensity for rapid flocculation within 10 to 135 min, resulting in the formation of large aggregates up to 1100 μm in diameter. The results indicated that the discharge of elevated plume concentrations (500 mg L–1) under an increased shear rate (G ≥ 2.4 s–1) would result in improved efficiency of sediment flocculation. Furthermore, particle transport model results suggested that even under typical deep-sea flow conditions (G ≈ 0.1 s–1), rapid deposition of particles could be expected, which would restrict heavy sediment blanketing (several centimeters) to a smaller fall-out area near the source, unless subsequent flow events resuspended the sediments. Planning for in situ tests of these model projections is underway

Description: Future mining of polymetallic nodules in the Clarion Clipperton Zone (Northeastern Pacific) is expected to affect all benthic ecosystems. The diversity, distribution, and environmental functions of microorganisms inhabiting abyssal sediments are barely understood. To understand consequences of deep-sea mining, experimental in vitro systems needs to be established to test hypotheses on the environmental impact of mining. For this, 40 bacterial strains, belonging to proteobacteria, actinobacteria and firmicutes were isolated from deep-sea sediments and nodules sampled at depths of ≥ 4000 m. Phenotypic characterization revealed a strong inter-species and moderate intra-species variability. Determination of metal minimum inhibitory concentrations indicated the presence of acute manganese-resistant bacteria such as Rhodococcus erythropolis (228.9 mM), Loktanella cinnabarina (57.2 mM), and Dietzia maris (14.3 mM) that might be suitable systems for testing the effects of release of microbes from nodules and their interactions with sediment particles in plumes generated during mining. Comparative genomic analysis indicated the presence of manganese efflux systems relevant for future transcriptomics or proteomics approaches with environmental samples and might serve in paving the way to develop model systems including representative organisms which are currently not cultivable. Monitoring deep-sea mining activity at abyssal depth is a challenge that has to be tackled. We proposed the use of API strips as a fast on-board methodology for bacterial monitoring as an indicator for sediment plume dispersions within the water column.

Description: Despite the technological advances of the last decades (e.g. ROVs, AUVs, cabled observatories), our knowledge of most deep-sea environments is still strongly limited by spatio-temporal sampling and observational capabilities. The novel Internet Operated Deep-Sea Crawler technology can provide high-frequency, multi-sensor data, during long-term deployments, 24/7 communication with researchers and broader spatial coverage (i.e. mobile platform) than fixed instrument installations. The crawler “Wally” is deployed at the Barkley Canyon methane hydrates site (NE Pacific, Canada; ~890 m depth) and connected to the Ocean Networks Canada NEPTUNE cabled observatory network (ONC; www.oceannetworks.ca). Here we present the environmental and biological datasets obtained from Wally instruments and cameras, during the first deployment phase (September 2010 to January 2015), as well as new features and preliminary results obtained since it was re-deployed (May 2016 – present). In addition to data provided by the standard payload of the crawler (i.e. ADCP, CTD, methane sensor, turbidity sensor and fluorometer), the hydrates community was video-monitored at different frequencies and timespans. Photomosaics were generated at two distinct locations, in order to map chemosynthetic bacterial mats and vesicomyid clam colonies covering the ~2-3 m high hydrate mounds, and document their temporal dynamics. The crawler followed the development of a deep-sea shell taphonomic experiment aiming to quantify biogenic carbon fluxes at the hydrates environment. The composition and diel activity patterns of the hydrates megafaunal community were studied with the use of linear video-transects conducted from February 2013 to April 2014. Since the summer of 2016, video-frames recorded at different locations of the site are analyzed for a biodiversity study and photomosaicing of the hydrate mounds continues, with 3D modelling of the mound structures also available as a new feature of the crawler deployed in May 2016. All data are archived in real-time and can be accessed online on the Ocean Networks Canada database. As deep-sea crawler technology and similar mobile, benthic platform technologies progress towards full operational autonomy, they will provide an even greater capacity for future monitoring and understanding of dynamic, extreme environments such as methane hydrate fields.

Description: Three benthic megafaunal species (i.e. sablefish Anoplopoma fimbria; pacific hagfish Eptatretus stoutii and a group of juvenile crabs) were tested for diel behavioral patterns at the methane hydrates site of Barkley Canyon (890 m depth), off Vancouver Island (BC, Canada). Fluctuations of animal counts in linear video-transects conducted with the Internet Operated Deep-Sea Crawler ªWallyº in June, July and December of 2013, were used as proxy of population activity rhythms. Count time series and environmental parameters were analyzed under the hypothesis that the environmental conditioning of activity rhythms depends on the life habits of particular species (i.e. movement type and trophic level). Nonlinear least squares modeling of biological time series revealed significant diel periods for sablefish in summer and for hagfish and crabs in December. Combined cross-correlation and redundancy (RDA) analyses showed strong relationships among environmental fluctuations and detected megafauna. In particular, sablefish presence during summer months was related to flow magnitude, while the activity of pacific hagfish and juvenile crabs in December correlated with change in chemical parameters (i.e. chlorophyll and oxygen concentrations, respectively). Waveform analyses of animal counts and environmental variables confirmed the phase delay during the 24 h cycle. The timing of detection of sablefish occurred under low flow velocities, a possible behavioral adaptation to the general hypoxic conditions. The proposed effect of chlorophyll concentrations on hagfish counts highlights the potential role of phytodetritus as an alternative food source for this opportunistic feeder. The juvenile crabs seemed to display a cryptic behavior, possibly to avoid predation, though this was suppressed when oxygen levels were at a minimum. Our results highlight the potential advantages such mobile observation platforms offer in multiparametric deep-sea monitoring in terms of both spatial and temporal resolution and add to the vastly understudied field of diel rhythms of deep-sea megafauna.

Description: The anthropogenic impact of polymetallic nodule harvesting in the Clarion-Clipperton Fracture Zone is expected to strongly affect the benthic ecosystem. To predict the long-term, industrial-scale impact of nodule mining on the deep-sea environment and to improve the reliability of the sediment plume model, information about the specific characteristics of deep-sea particles is needed. Discharge simulations of mining-related fine-grained (median diameter ≈ 20 μm) sediment plumes at concentrations of 35–500 mg L–1 (dry weight) showed a propensity for rapid flocculation within 10 to 135 min, resulting in the formation of large aggregates up to 1100 μm in diameter. The results indicated that the discharge of elevated plume concentrations (500 mg L–1) under an increased shear rate (G ≥ 2.4 s–1) would result in improved efficiency of sediment flocculation. Furthermore, particle transport model results suggested that even under typical deep-sea flow conditions (G ≈ 0.1 s–1), rapid deposition of particles could be expected, which would restrict heavy sediment blanketing (several centimeters) to a smaller fall-out area near the source, unless subsequent flow events resuspended the sediments. Planning for in situ tests of these model projections is underway

Description: Knowledge on basic biological functions of organisms is essential to understand not only the role they play in the ecosystems but also to manage and protect their populations. The study of biological processes, such as growth, reproduction and physiology, which can be approached in situ or by collecting specimens and rearing them in aquaria, is particularly challenging for deep-sea organisms like cold-water corals. Field experimental work and monitoring of deep-sea populations is still a chimera. Only a handful of research institutes or companies has been able to install in situ marine observatories in the Mediterranean Sea or elsewhere, which facilitate a continuous monitoring of deep-sea ecosystems. Hence, today’s best way to obtain basic biological information on these organisms is (1) working with collected samples and analysing them post-mortem and / or (2) cultivating corals in aquaria in order to monitor biological processes and investigate coral behaviour and physiological responses under different experimental treatments. The first challenging aspect is the collection process, which implies the use of oceanographic research vessels in most occasions since these organisms inhabit areas between ca. 150 m to more than 1000 m depth, and specific sampling gears. The next challenge is the maintenance of the animals on board (in situations where cruises may take weeks) and their transport to home laboratories. Maintenance in the home laboratories is also extremely challenging since special conditions and set-ups are needed to conduct experimental studies to obtain information on the biological processes of these animals. The complexity of the natural environment from which the corals were collected cannot be exactly replicated within the laboratory setting; a fact which has led some researchers to question the validity of work and conclusions drawn from such undertakings. It is evident that aquaria experiments cannot perfectly reflect the real environmental and trophic conditions where these organisms occur, but: (1) in most cases we do not have the possibility to obtain equivalent in situ information and (2) even with limitations, they produce relevant information about the biological limits of the species, which is especially valuable when considering potential future climate change scenarios. This chapter includes many contributions from different authors and is envisioned as both to be a practical “handbook” for conducting cold-water coral aquaria work, whilst at the same time offering an overview on the cold-water coral research conducted in Mediterranean laboratories equipped with aquaria infrastructure. Experiences from Atlantic and Pacific laboratories with extensive experience with cold-water coral work have also contributed to this chapter, as their procedures are valuable to any researcher interested in conducting experimental work with cold-water corals in aquaria. It was impossible to include contributions from all laboratories in the world currently working experimentally with cold-water corals in the laboratory, but at the conclusion of the chapter we attempt, to our best of our knowledge, to supply a list of several laboratories with operational cold-water coral aquaria facilities.

Description: This work aimed to explore evaluated the effects of the increased of hydrostatic pressure on a defined bacterial community on aggregates formed from an axenic culture of marine diatoms by simulating sedimentation to the deep sea by increase of hydrostatic pressure up to 30 bar (equivalent to 3000 m water depth) against control at ambient surface pressure. Our hypothesis was that microbial colonization and community composition and thus microbial OM turnover is greatly affected by changes in hydrostatic pressure during sinking to the deep ocean.