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  • 2005-2009  (14)
  • 2006  (14)
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  • 2005-2009  (14)
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  • 1
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    Allelochemicals of the marine dinoflagellate Alexandrium spp. - a means of excluding protistan grazers and competitors? (2006)
    In:  EPIC3Vortrag CO-Symposium, 16.-17.2.06, Hamburg.
    Details
    Publication Date: 2019-07-16
    Repository Name: EPIC Alfred Wegener Institut
    Type: Conference , notRev
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  • 2
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    Allelopathic effects of bioactive compounds produced by harmful algae (2006)
    In:  EPIC3invited plenary talk, 12th International Conference on Harmful Algae, Copenhagen, DenmarkSeptember 2006., 4
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    Publication Date: 2019-07-16
    Repository Name: EPIC Alfred Wegener Institut
    Type: Conference , notRev
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  • 3
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    Allelochemical Interactions of marine Protists (2006)
    In:  EPIC336th Annual Conference of the Ecological Society of Germany, Switzerland and Austria (GfÖ), 11-15 September 2006, Bremen, Germany.
    Details
    Publication Date: 2019-07-16
    Repository Name: EPIC Alfred Wegener Institut
    Type: Conference , notRev
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  • 4
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    Genetic differentiation and phenotypic characteristics of geographically separated populations of the Alexandrium tamarense North American ribotype (2006)
    In:  EPIC312th International Conference on Harmful Algae, 4-8 Sept., Copenhagen, Denmark.
    Details
    Publication Date: 2019-07-16
    Description: The Alexandrium tamarense species complex contains prominent paralytic shellfish poisoning (PSP) toxin producers that can be further discriminated either on the basis of morphological characteristics or by DNA sequence divergence. The North American clade, as defined by its LSU ribosomal DNA sequence, is the most widely distributed representative of the PSP toxin-producing A. tamarense clades. Populations of this clade cause recurrent blooms in many regions of the world. Natural populations from North America, Northern Europe and Japan exhibit notable genetic differentiation that can be detected by molecular markers with different resolution properties. Whereas molecular sequence analysis of ribosomal DNA yields only a coarse resolution pattern of regional subclades, mitochondrial DNA sequences and microsatellites, as well as Amplified Fragment Length Polymorphism (AFLP) analysis, allow the estimation of genetic differentiation between contiguous populations. No congruence of any of the genetic markers were found between the expression of PSP toxin phenotypes or allelochemical properties that can affect grazers or competing algal species, but inter-population differences in PSP toxin profile were apparent on a broad geographical scale. The variable expression of the allelopathic phenotype within a population from Northern Europe was used to experimentally test the protective benefit of allelochemical properties on bloom formation.
    Repository Name: EPIC Alfred Wegener Institut
    Type: Conference , notRev
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  • 5
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    Genetic and phenotypic differences among species and strains of potential fish-killing raphidophytes in the Mediterranean (2006)
    In:  EPIC312th International Conference on Harmful Algae, 4-8 Sep., Copenhagen, Denmark.
    Details
    Publication Date: 2019-07-16
    Description: Dense raphidophyte blooms are a cause of mass fish mortalities in coastal areas throughout the world. The recent dramatic global increase in aquaculture, especially fish farming, has led to a heightened requirement for knowledge on characterization and toxigenicity of raphidophyte blooms. In the Mediterranean, raphidophytes form annual blooms in coastal waters, thereby posing a threat to fish aquaculture. We investigated a mixed bloom event involving the raphidophytes Chattonella sp. and Fibrocapsa sp. on the Adriatic coast of Italy, including molecular genetic, morphological and ecological aspects. Light and electron microscopy revealed one of species as Chattonella subsalsa as described by Biecheler (1936). Nevertheless, genetic markers for nuclear and ribosomal DNA (LSU, SSU, ITS, psaA and RubisCo) differentiated this Chattonella, and with less distinctness, Fibrocapsa sp., from other known strains of the respective genera. Phenotypic differences among isolated clones were determined from profiles of polyunsaturated fatty acids (PUFAs) and activity of reactive oxygen species (ROS), which are putatively responsible for ichthyotoxicity. Strains were bioassayed with a fish erythrocyte lytic assay and the brine shrimp Artemia salina test. The relationship (if any) between toxicity and phenotypic and genotypic characteristics is complex and not yet resolved.
    Repository Name: EPIC Alfred Wegener Institut
    Type: Conference , notRev
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  • 6
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    Chemical Ecology of Toxic Algae (2006)
    In:  EPIC3Tagung der Sektion Phykologie in der Deutschen Botanischen Gesellschaft, 28.-31. Aug. 2006, Helgoland.
    Details
    Publication Date: 2016-01-12
    Description: Toxic marine algae increasingly cause problems worldwide by forming so called harmful algal blooms (HABs). Although growth, taxonomic composition and diversity, ecophysiology and biomass transfer are well understood, few is known about the chemical interactions on species level, which are responsible for the regulation of growth, competitive factors and species succession. The ecological chemistry group at the AWI starts an interdisciplinary approach towards the understanding of these interactions of single protist species. The interdisciplinary approach consists of competition and survival experiments for the detection and investigation of allelochemical interactions among protist species, genetic and molecular biological methods for the analysis of gene expression and function of involved genes, and finally analytical chemical methods for the detection and quantitation of secondary metabolites that mediate these effects. A short overview over some currently ongoing projects will be given.
    Repository Name: EPIC Alfred Wegener Institut
    Type: Conference , notRev
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  • 7
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    MOLECULAR INVESTIGATIONS ON THE TOXIC MARINE DINOFLAGELLATE ALEXANDRIUM MINUTUM (2006)
    In:  EPIC312th International Conference on Harmful Algae, 4.-8. Sept. 2006, Copenhagen, Denmark..
    Details
    Publication Date: 2019-07-16
    Description: Alexandrium minutum is a widely distributed HAB dinoflagellate that typically produces PSP neurotoxins (gonyautoxins 1,2,3,4), as well as poorly characterized allelochemicals. Within the EU-Project ESTTAL (Expressed Sequence Tags of Toxic Algae), we aim to identify candidate genes and processes putatively involved in toxicity and allelochemical interactions, and in growth regulation and stress responses. To obtain RNA samples linked with physiological status, batch cultures of A. minutum were grown under different environmental conditions, yielding differences in growth, PSP toxin production, and allelochemical potency. As a basis for gene expression analysis, we generated a normalized cDNA library, from which about 10,000 expressed sequence tags (ESTs) were generated. Annotation of these data yielded first insights into the genome of A. minutum. Based on the EST library, we designed an oligonucleotide microarray, which will be used to screen for differences in gene expression among the differently treated A. minutum cultures. Here we present the results of the EST library analysis, the design of the microarray, and physiological data of cultures grown for gene expression analysis.
    Repository Name: EPIC Alfred Wegener Institut
    Type: Conference , notRev
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  • 8
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    Phagotrophy in Harmful Algae (2006)
    In:  EPIC3in: Ecology of Harmful Algae. Granéli, E., Turner, J.T. (Eds.). Ecological Studies Vol. 189, Springer, Berlin, pp. 177-187
    Details
    Publication Date: 2019-07-17
    Repository Name: EPIC Alfred Wegener Institut
    Type: Inbook , peerRev
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  • 9
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    Revelaing the allelochemical potential of Alexandrium ostenfeldii, a marine dinoflagellate (2006)
    In:  EPIC336th Annual Conference Gesellschaft für Ökologie, 11.-15. Sept. 2006, Bremen.
    Details
    Publication Date: 2019-07-16
    Description: Chemical ecology in marine systems is less understood than in terrestrial systems. Nevertheless, even such simple organisms as unicellular marine microalgae use secondary metabolites for defense, prey acquisition, induction of sexuality or life cycle transitions. With the example of the toxic species Alexandrium ostenfeldii we apply an interdisciplinary approach using molecular, biological, toxicological and analytical tools towards the functional understanding of secondary metabolites of A. ostenfeldii as well as its ecological effects. Information on the expressed genome has been gathered by creating expressed sequence tag (EST) libraries and comparing the sequence data with known sequences from gene bank. Polyketide synthase (PKS) genes have been identified, which are responsible for the biosynthesis of spirolides, a class of macrocyclic imine neurotoxins synthesized by A. ostenfeldii. This group of toxins, including new analogues has been detected by liquid chromatography-tandem mass spectrometry coupling (LC-MS/MS). Spirolide cytotoxicity has been examined with mouse neuroblastoma cell cultures and assays on alleochemical effects of extracellular metabolites of A. ostenfeldii on other marine protists have been performed. This interdisciplinary research has contributed substantially to understanding the relationship between genetic expression of macrocyclic imine toxins and their toxicological mode of action.
    Repository Name: EPIC Alfred Wegener Institut
    Type: Conference , notRev
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  • 10
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    Primary Productivity in the German Bight (1994-1996) (2006)
    In:  EPIC3Estuaries, 29(1), pp. 4-23
    Details
    Publication Date: 2019-07-17
    Repository Name: EPIC Alfred Wegener Institut
    Type: Article , isiRev
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