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  • 1
    Book
    Book
    Synthesis and new conception of North Sea Research (SYCON) : Working group 7: phytoplankton (2001)
    Hamburg : Zentrum für Meeres- und Klimaforschung
    Details Availability
    Keywords: Phytoplankton ; North Sea ; marine biology ; oceanology
    Description / Table of Contents: Ohne Kurzreferat
    Type of Medium: Book
    Pages: 111 S , graph. Darst.
    Series Statement: Berichte aus dem Zentrum für Meeres- und Klimaforschung 9
    Language: English
    Note: Literaturverz. S. 85 - 111
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  • 2
    Online Resource
    Online Resource
    RIPAZA - Risikopotential von Azaspirosäuren-Muschelvergiftung (AZP) in chinesischen Küstengewässern und der Nordsee unter sich ändernden Umweltbedingungen : Abschlussbericht : Berichtszeitraum: 1. August 2017-31. Oktober 2020 (2021)
    [Bremerhaven] : [AWI Bremerhaven]
    Details Availability
    Keywords: Forschungsbericht ; Muschelvergiftung
    Type of Medium: Online Resource
    Pages: 1 Online-Ressource (32 Seiten, 4,19 MB) , Illustrationen, Diagramme
    URL: https://doi.org/10.2314/KXP:1805555081
    DOI: 10.2314/KXP:1805555081
    Language: German
    Note: Förderkennzeichen BMBF 03F0763A , Unterschiede zwischen dem gedruckten Dokument und der elektronischen Ressource können nicht ausgeschlossen werden
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  • 3
    Online Resource
    Online Resource
    ARCHEMHAB : interactions and feedback mechanisms between hydrography, geochemical signatures and microbial ecology, with a focus on HAB species diversity, biogeography and dynamics : Cruise No. MSM 21/3 - July 25 - August 10, 2012 - Nuuk (Greenland) - Reykjavik (Iceland) (2016)
    Bremen : DFG-Senatskommission für Ozeanographie c/o MARUM - Zentrum für Marine Umweltwissenschaften, Universität Bremen
    Details Availability
    Type of Medium: Online Resource
    Pages: 1 Online-Ressource (49 Seiten)
    Series Statement: Maria S. Merian-Berichte MSM 21/3
    URL: https://doi.org/10.2312/cr_msm21_3
    DOI: 10.2312/cr_msm21_3
    Language: English
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  • 4
    Electronic Resource
    Electronic Resource
    Interactions between Planktonic Microalgae and Protozoan Grazers (2004)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 51 (2004), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: . For an algal bloom to develop, the growth rate of the bloom-forming species must exceed the sum of all loss processes. Among these loss processes, grazing is generally believed to be one of the more important factors. Based on numerous field studies, it is now recognized that microzooplankton are dominant consumers of phytoplankton in both open ocean and coastal waters. Heterotrophic protists, a major component of microzooplankton communities, constitute a vast complex of diverse feeding strategies and behavior which allow them access to even the larger phytoplankton species. A number of laboratory studies have shown the capability of different protistan species to feed and grow on bloom-forming algal species. Because of short generation times, their ability for fast reaction to short-term variation in food conditions enables phagotrophic protists to fulfill the function of a heterotrophic buffer, which might balance the flow of matter in case of phytoplankton blooms. The importance of grazing as a control of microalgae becomes most apparent by its failure; if community grazing controls initial stages of bloom development, there simply is no bloom. However, if a certain algal species is difficult to graze, e.g. due to specific defense mechanisms, reduced grazing pressure will certainly favor bloom development. The present contribution will provide a general overview on the interactions between planktonic microalgae and protozoan grazers with special emphasis on species-specific interactions and algal defense strategies against protozoan grazers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.2004.tb00540.x
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    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Distribution of carbon among photosynthetic end products in the bloom-forming arctic diatom Thalassiosira antarctica COMBER (1989)
    Springer
    Polar biology 10 (1989), S. 231-238 
    Details
    ISSN: 1432-2056
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Investigations of the bloom-forming arctic diatom Thalassiosira antarctica COMBER show that at all temperature and light conditions tested, the 14C-incorporation rates in lipids averaged 20% and in carbohydrates 12%. The percentage of 14C in proteins and small metabolites ranged from 30% to 50% respectively, with contrary trends. The production rate of protein per generation time was not temperature and light dependent. The changes in percent carbon incorporated into proteins may be due to enhanced metabolite synthesis at high irradiances and/or temperatures above 0° C.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00238500
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  • 6
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    NMR spectra of 16-desmethylGYMD (1), GYM E (2), 20-Hydroxy-13,19-didesMethylSPXC (10) and 20-Hydroxy-13,19-didesMethylSPXD (11) (2018)
    PANGAEA
    In:  Supplement to: Zurhelle, Christian; Nieva, Joyce; Tillmann, Urban; Krock, Bernd; Tebben, Jan (2018): Identification of novel gmnodimines and spirolides from the marine dinoflagellate Alexandrium ostenfeldii. 16(11), 446, https://doi.org/10.3390/md16110446
    Details
    Publication Date: 2023-03-16
    Description: The strain of Alexandrium ostenfeldii was isolated from a single cell from Ouwerkerkse Kreek.
    Keywords: AWI_EcolChem; BIO; Biology; Ecological Chemistry @ AWI; OuwerkerkseKreek
    Type: Dataset
    Format: application/zip, 386.9 MBytes
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    DATA PANGAEA
  • 7
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    Data files of figures 1, 2 and 3 (2011)
    PANGAEA
    In:  Supplement to: Ma, Haiyan; Krock, Bernd; Tillmann, Urban; Cembella, Allan (2010): Towards characterization of lytic compound(s) produced by Alexandrium tamarense. Proceedings of the 13th International Conference on Harmful Algae, Ed. Ho, K. et al., International Society for the Study of Harmful Algae and Intergovernmental Oceanographic Commission of UNESCO, Hong Kong, 2008, China, 142-146, hdl:10013/epic.36232.d001
    Details
    Publication Date: 2023-01-13
    Description: We investigated optimal conditions for characterization of bioactivity of lytic compound(s) excreted by Alexandrium tamarense based on a cell-bioassay system. Allelochemical response of the cryptophyte Rhodomonas salina indicated the presence oflytic compound(s) in a reliable and reproducible way and allows for quantification of this lytic effect. The parameters tested were the incubation time of putatively lytic extracts or fractions with the target organism R. salina, different techniques for cell harvest from A. tamarense cultures and the optimal harvest time. A three hour incubation time was found to be optimal to yield a rapid response while accurately estimating effective concentration (ECso) values. Harvest of A. tamarense cultures by filtration resulted in loss of lytic activity in most cases and centrifugation was most efficient in terms of recovery of lytic activity. Maximum yield of extracellular lytic activity of A. tamarense cultures was achieved in the stationary phase. Such optimized bioassay guided fractionation techniques are a valuable asset in the isolation and eventual stmctural elucidation of the unknown lytic substances.
    Type: Dataset
    Format: application/zip, 368.1 kBytes
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  • 8
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    Files related to figures 1 to 13 (2011)
    PANGAEA
    In:  Supplement to: Ma, Haiyan; Krock, Bernd; Tillmann, Urban; Cembella, Allan (2009): Preliminary characterization of extracellular allelochemicals of the toxic marine dinoflagellate Alexandrium tamarense using a Rhodomonas salina bioassay. Marine Drugs, 7(4), 497-522, https://doi.org/10.3390/md7040497
    Details
    Publication Date: 2023-01-13
    Description: Members of the marine dinoflagellate genus Alexandrium are known to exude allelochemicals, unrelated to well-known neurotoxins (PSP-toxins, spirolides), with negative effects on other phytoplankton and marine grazers. Physico/chemical characterization of extracellular lytic compounds of A. tamarense, quantified by Rhodomonas salina bioassay, showed that the lytic activity, and hence presumably the compounds were stable over wide ranges of temperatures and pH and were refractory to bacterial degradation. Two distinct lytic fractions were collected by reversed-phase solid-phase extraction. The more hydrophilic fraction accounted for about 2% of the whole lytic activity of the A. tamarense culture supernatant, while the less hydrophilic one accounted for about 98% of activity. Although temporal stability of the compounds is high, substantial losses were evident during purification. Lytic activity was best removed from aqueous phase with chloroform-methanol (3:1). A “pseudo-loss” of lytic activity in undisturbed and low-concentrated samples and high activity of an emulsion between aqueous and n-hexane phase after liquid-liquid partition are strong evidence for the presence of amphipathic compounds. Lytic activity in the early fraction of gel permeation chromatography and lack of activity after 5 kD ultrafiltration indicate that the lytic agents form large aggregates or macromolecular complexes.
    Type: Dataset
    Format: application/zip, 843.6 kBytes
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  • 9
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    Files of figures 2, 3 and table (2011)
    PANGAEA
    In:  Supplement to: Ma, Haiyan; Krock, Bernd; Tillmann, Urban; Bickmeyer, Ulf; Graeve, Martin; Cembella, Allan (2011): Mode of action of membrane-disruptive lytic compounds from the marine dinoflagellate Alexandrium tamarense. Toxicon, 58(3), 247-258, https://doi.org/10.1016/j.toxicon.2011.06.004
    Details
    Publication Date: 2023-01-13
    Description: Certain allelochemicals of the marine dinoflagellate Alexandrium tamarense cause lysis of a broad spectrum of target protist cells but the lytic mechanism is poorly defined. We first hypothesized that membrane sterols serve as molecular targets of these lytic compounds, and that differences in sterol composition among donor and target cells may cause insensitivity of Alexandrium and sensitivity of targets to lytic compounds. We investigated Ca2+ influx after application of lytic fractions to a model cell line PC12 derived from a pheochromocytoma of the rat adrenal medulla to establish how the lytic compounds affect ion flux associated with lysis of target membranes. The lytic compounds increased permeability of the cell membrane for Ca2+ ions even during blockade of Ca2+ channels with cadmium. Results of a liposome assay suggested that the lytic compounds did not lyse such target membranes non-specifically by means of detergent-like activity. Analysis of sterol composition of isolates of A. tamarense and of five target protistan species showed that both lytic and non-lytic A. tamarense strains contain cholesterol and dinosterol as major sterols, whereas none of the other tested species contain dinosterol. Adding sterols and phosphatidylcholine to a lysis bioassay with the cryptophyte Rhodomonas salina for evaluation of competitive binding indicated that the lytic compounds possessed apparent high affinity for free sterols and phosphatidylcholine. Lysis of protistan target cells was dose-dependently reduced by adding various sterols or phosphatidylcholine. For three tested sterols, the lytic compounds showed highest affinity towards cholesterol followed by ergosterol and brassicasterol. Cholesterol comprised a higher percentage of total sterols in plasma membrane fractions of A. tamarense than in corresponding whole cell fractions. We conclude therefore that although the molecular targets of the lytic compounds are likely to involve sterol components of membranes, A. tamarense must have a complex self-protective mechanism that still needs to be addressed.
    Type: Dataset
    Format: application/zip, 164.3 kBytes
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  • 10
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    Cell count time series of Azadinium spinosum, Azadinium poporum and Amphidoma languida at Cuxhaven, Helgoland, Sylt, Wilhelmshaven and Scapa Flow between 2015 and 2019 (2021)
    PANGAEA
    Details
    Publication Date: 2023-01-30
    Description: The data represent species counts (cells L-1) of the three AZA-producing dinoflagellate species Azadinium spinosum, Az. poporum and Amphidoma languida (all members of the taxonomic family Amphidomataceae) of water samples taken during in total six different field expeditions on several research vessels (RV Heincke, RV Uthörn, RV Polarstern) and on in total five stationary sampling stations (Scapa Flow/Scotland, Cuxhaven/Germany, Helgoland/Germany, Wilhelmshaven/Germany, Sylt/Germany) between 2015 and 2019. The water samples have been taken using Niskin bottles (on research vessels attached to a CTD). After DNA extraction, the species cell numbers have been calculated by quantitative PCR (qPCR) analysis using respective standard curves. These samples gained from different geographical areas in the eastern North Atlantic have been analyzed as part of the RIPAZA Project (funded by the German BMBF; in cooperation with the Third Institute of Oceanography, Xiamen/China) and the results are presented and discussed in the doctoral thesis of Stephan Wietkamp (Suppl.Tab.S6, Suppl.Tab.S7). Aim of the project and especially of this data set was to provide first reference data on the biogeography (geographical distribution and seasonality) of toxigenic Amphidomataceae in the eastern North Atlantic.
    Keywords: Amphidoma languida; Azadinium; Azadinium poporum; Azadinium spinosum; Azaspiracids; Cuxhaven_WS; DATE/TIME; Dinoflagellates; DNA; Field observation; Germany; Helgoland_WS; LATITUDE; Location; LONGITUDE; North Atlantic; qPCR; QPCR; Quantitative real-time PCR (qPCR); ScapaFlow_WS; Scotland; Sylt_WS; Water sample; Wilhelmshaven_WS; WS
    Type: Dataset
    Format: text/tab-separated-values, 980 data points
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