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  • 1
    Electronic Resource
    Electronic Resource
    Value creation and sharing among universities, biotechnology and pharma (2003)
    [s.l.] : Nature Publishing Group
    Nature biotechnology 21 (2003), S. 618-624 
    Details
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] The three decades since the first breakthroughs in biotechnology and the subsequent passage of the Bayh-Dole Act have seen an enormous rise in the direct participation of universities and academic scientists in the development of novel medicines, diagnostics and discovery techniques. Over this same ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nbt0603-618
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  • 2
    Electronic Resource
    Electronic Resource
    Biotechnology financing dilemmas and the role of special purpose entities (2004)
    [s.l.] : Nature Publishing Group
    Nature biotechnology 22 (2004), S. 271-277 
    Details
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] Biotech firms attempting to make the transition from early-stage R&D to fully integrated companies with robust pipelines face a daunting financial challenge. Given that the extraordinary costs and time required to bring not one but a series of successful compounds to market cannot be sustained ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nbt0304-271
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  • 3
    Electronic Resource
    Electronic Resource
    Gold in the ivory tower: equity rewards of outlicensing (2006)
    [s.l.] : Nature Publishing Group
    Nature biotechnology 24 (2006), S. 509-515 
    Details
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] Since the invention of recombinant DNA techniques, monoclonal antibodies and beyond, biotech companies have relied upon academic discoveries as the foundation of their technology value. University-biotech licensing agreements have become more complex over time, but typically include one or more of ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nbt0506-509
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  • 4
    Electronic Resource
    Electronic Resource
    Expression of the Talaromyces flavus glucose oxidase gene in cotton and tobacco reduces fungal infection, but is also phytotoxic (1999)
    Springer
    Molecular breeding 5 (1999), S. 219-232 
    Details
    ISSN: 1572-9788
    Keywords: disease resistance ; glucose oxidase ; Gossypium hirsutum ; Nicotiana tabacum ; Talaromyces flavus ; transgenic plant
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Glucose oxidase secreted by the fungus Talaromyces flavus generates, in the presence of glucose, hydrogen peroxide that is toxic to phytopathogenic fungi responsible for economically important diseases in many crops. A glucose oxidase gene from T. flavus, was modified with a carrot extensin signal peptide and fused to either a constitutive or root-specific plant promoter. T1 tobacco plants expressing the enzyme constitutively were protected against infection by the seedling pathogen Rhizoctonia solani. Constitutive expression in tobacco was associated with reduced root growth, and slow germination on culture medium, and with reduced seed set in glasshouse conditions. Several independent transformed cotton plants with a root-specific construct expressed high glucose oxidase activity in the roots, excluding the root tip. Selected T3 homozygous lines showed some protection against the root pathogen, Verticillium dahliae, but not against Fusarium oxysporum. High levels of glucose oxidase expression in cotton roots were associated with reduced height, seed set and seedling germination and reduced lateral root formation. If this gene is to be of value for crop protection against pathogens it will require precise control of its expression to remove the deleterious phenotypes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009625801909
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  • 5
    Electronic Resource
    Electronic Resource
    Surrogate transformation of perennial ryegrass,Lolium perenne, using genetically modifiedAcremonium endophyte (1992)
    Springer
    Molecular genetics and genomics 233 (1992), S. 1-9 
    Details
    ISSN: 1617-4623
    Keywords: Acremonium sp. ; Transformation ; Chromosomal karyotype ; Surrogate grass transformation ; uidA andhph genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Conditions have been developed for transforming protoplasts of the perennial ryegrass endophyteAcremonium strain 187BB. Unlike most other ryegrass endophytes, this strain does not produce the lolitrem B neurotoxin and is therefore suitable as a host for surrogate introduction of foreign genes into grasses. Transformation frequencies of 700–800 transformants/μg DNA were obtained for both linear and circular forms of pAN7-1, a hygromycin (hph) resistant plasmid. Up to 80% of the linear transformants were stable on further culturing but only 25% of the circular transformants retained hygromycin resistance. Integration of pAN7-1 into the genome was confirmed by Southern blotting and probing of genomic digests of transformant DNA. Both single and tandemly repeated copies of the plasmid were found in the genome and both the number and sites of integration varied among the transformants. At least 13 chromosomes were identified in 187BB using contour-clamped homogeneous electric field (CHEF) gel electrophoresis. Probing of Southern blots of these gels confirmed that pAN7-1 had integrated into different chromosomes. The β-glucuronidase (GUS) gene,uidA, was also introduced into 187BB by co-transformation of pNOM-2 with pAN7-1. GUS activity was detected by growing the transformants on plates containing 5-bromo-4-chloro-3-indolyl β-D-glucuronic acid and by enzyme assays of mycelial extracts. Severalhph- anduidA-containing transformants were reintroduced into ryegrass seedlings and expression of GUS visualized in vivo, demonstrating that 187BB can be used as a surrogate host to introduce foreign genes into perennial ryegrass. Molecular analysis of fungal isolates from the leaf sheath confirmed that the pattern of pAN7-1 and pNOM-2 hybridizing fragments was identical to that observed in the fungus used as inoculum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00587554
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