ISSN:
0006-3525
Keywords:
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
A method is presented which allows one to calculate a distribution of sedimentation coefficients from the boundries of sedimentation-velocity experiments with mono- or pauci-disperse solutes. With two solutes differences in S value as small as 20% can be resolved. In absence of heterogeneity and concentration-dependent effects, the analysis also provides values for the diffusion coefficient within an accuracy of -10 to +5%. Tests with both simulated data and ultracentrifugation experiments on short DNA fragments show the value and the limitations of the method.
Additional Material:
10 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bip.1978.360170602
