Publication Date:
2015-11-28
Description:
Publication date: Available online 26 November 2015 Source: FEBS Letters Author(s): N. Caporarello, M. Salmeri, M. Scalia, C. Motta, C. Parrino, L. Frittitta, M. Olivieri, M.A. Toscano, C.D. Anfuso, G. Lupo Cytosolic PLA 2 (cPLA 2 ) and Ca 2+ -independent PLA 2 (iPLA 2 ) play a significant role in insulin β-cells secretion. Bacterial infections may be responsible of the onset of diabetes. The mechanism by which S. aureus infection of INS-1 cells alters glucose-induced insulin secretion has been examined. After acute infection, insulin secretion and PLA 2 activities significantly increased. Moreover, increased expressions of phospho-cPLA 2 , phospho-PKCα and phospho-ERK 1/2 were observed. Chronic infection causes a decrease in insulin release and a significant increase of iPLA 2 and COX-2 protein expression. Moreover, insulin secretion in infected cells could be restored using specific si RNAs against iPLA 2 isoform and specific COX-2 inhibitor.
Print ISSN:
0014-5793
Electronic ISSN:
1873-3468
Topics:
Biology
,
Chemistry and Pharmacology
,
Physics
