Publikationsdatum:
2014-11-29
Beschreibung:
Publication date: Available online 27 November 2014 Source: FEBS Open Bio Author(s): Yeo Cho Yoon , Sung-Hee Kim , Min Jung Kim , Hye Jeong Yang , Mee-Ra Rhyu , Jae-Ho Park This study investigated the effects of an ethanol extract of black pepper and its constituent, piperine, on odorant-induced signal transduction in non-chemosensory cells. An ethanol extract of black pepper decreased eugenol-induced cAMP and calcium levels in preadipocyte 3T3-L1 cells with no toxicity. Phosphorylation of CREB (cAMP response element-binding protein) was down-regulated by the black pepper extract. The concentration (133.8 mg/g) and retention time (5.5 min) of piperine in the ethanol extract were quantified using UPLC-MS/MS. Pretreatment with piperine decreased eugenol-induced cAMP and calcium levels in 3T3-L1 cells. Piperine also decreased the phosphorylation of CREB, which is up-regulated by eugenol. These results suggest that piperine inhibits the eugenol-induced signal transduction pathway through modulation of cAMP and calcium levels and phosphorylation of CREB in non-chemosensory cells.
Digitale ISSN:
2211-5463
Thema:
Biologie
