In:
Cold Spring Harbor Protocols, Cold Spring Harbor Laboratory, Vol. 2012, No. 2 ( 2012-02), p. pdb.prot067942-
Kurzfassung:
This article describes a procedure for conducting crosses between different strains of Ectocarpus . Crossing gametophytes to obtain the sporophyte generation is the most technically challenging stage of this process because diploid sporophytes have to be distinguished from the haploid partheno-sporophytes that result from the parthenogenetic germination of unfused gametes. This requires careful monitoring of the progeny of the genetic cross until they have developed sufficiently to be transferred to a separate Petri dish. Genetic crosses allow several classical genetic methodologies to be applied in Ectocarpus , including allelic complementation tests, backcrosses, combination of different genetic mutations, and outcrosses to create mapping populations.
Materialart:
Online-Ressource
ISSN:
1940-3402
,
1559-6095
DOI:
10.1101/pdb.prot067942
Sprache:
Englisch
Verlag:
Cold Spring Harbor Laboratory
Publikationsdatum:
2012
