ISSN:
1610-739X
Keywords:
Keywords: Sweet potato leaf curl virus, begomovirus, partial purification, western blotting.
Source:
Springer Online Journal Archives 1860-2000
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Sweet potato leaf curl virus (SPLCV) is a possible member of the genus Begomovirus; however, the presence of typical geminate particles in sap has not been confirmed. We attempted to observe SPLCV virions by partially purifying the virus using an enzyme-assisted procedure. The observed virions in the partially purified preparations were typical geminate particles with a size of ca. 18×30nm. This virus preparation was subjected to western blot analysis using antisera against Bean golden mosaic virus (BGMV) and Mungbean yellow mosaic virus (MYMV). SPLCV reacted with both antisera. Specific bands appeared to be slightly larger than the 30-kDa marker protein and were considered to be SPLCV coat protein. This western blot analysis revealed for the first time a serelogical relationship between SPLCV and the two well-characterized begomoviruses.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/PL00012942
