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  • 1
    Electronic Resource
    Electronic Resource
    Semipersistency of Myzus persicae Transmission of Cucumoviruses Systemically Infecting Leguminous Plants (2000)
    Springer
    Journal of general plant pathology 66 (2000), S. 64-67 
    Details
    ISSN: 1610-739X
    Keywords: Keywords: Cucumoviruses, leguminous plants, semipersistent transmission, Myzus persicae.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Sequential transmission tests of Peanut stunt virus (PSV) and Cucumber mosaic virus (CMV) systemically infecting common bean, Phaseolus vulgaris, were conducted using Myzus persicae allowed to fast for 2 hr and then to acquisition feed on infected common bean plants or purified virus for 10 min. In the sequential transmission tests using either one or 10 aphids per assay plant, three isolates of PSV (J,S,Y5) and one of CMV (V) were transmitted from and to common bean up to a third or fourth inoculation access. Many aphids transmitted these viruses to two or three plants. Purified viruses of PSV-S and CMV-V were also transmitted up to a third or second inoculation access at low percentage. On tobacco, Nicotiana tabacum, aphids transmitted PSV-S and CMV-V only in the first inoculation access, although PSV-S was transmitted to only one plant in the fourth and fifth inoculation access. These viruses may be transmitted in two phases by aphids, depending on the plant species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00012922
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Genomic Structure of a Geminivirus in the Genus Begomovirus from Yellow Vein-affected Eupatorium makinor (2000)
    Springer
    Journal of general plant pathology 66 (2000), S. 176-181 
    Details
    ISSN: 1610-739X
    Keywords: Keywords: monopartite begomovirus, sequence comparison, conserved motifs, Eupatorium makinoi.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: A virus from yellow vein-affected Eupatorium makinoi was tentatively designated as Eupatorium-infecting geminivirus (EuGV) on the basis of whitefly transmissibility, electron microscopic observation of geminate particles in sap and symptomatology. EuGV-speciflc DNA fragments were obtained by polymerase chain reaction (PCR). Based on the restriction analysis of the PCR products, EuGV was suggested to have a monopartite genome. We determined the putative, complete nucleotide sequence of the EuGV genome which is comprised of 2766 nucleotides. The EuGV sequence had two virus sense open reading frames (ORF)(V1, V2), four complementary sense ORFs (C1–C4) and a non-coding region termed the intergenic region. This genome structure is quite similar to other monopartite begomoviruses already reported. The nucleotide and amino acid sequences were compared with other begomoviruses. The results supported the conclusion that EuGV is distinct and divergent from other begomoviruses, whereas potential sequence motifs reported in other geminiviruses are well conserved.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00012941
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Geminate Particle Morphology of Sweet Potato Leaf Curl Virus in Partially Purified Preparation and Its Serological Relationship to Two Begomoviruses by Western Blotting (2000)
    Springer
    Journal of general plant pathology 66 (2000), S. 182-184 
    Details
    ISSN: 1610-739X
    Keywords: Keywords: Sweet potato leaf curl virus, begomovirus, partial purification, western blotting.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Sweet potato leaf curl virus (SPLCV) is a possible member of the genus Begomovirus; however, the presence of typical geminate particles in sap has not been confirmed. We attempted to observe SPLCV virions by partially purifying the virus using an enzyme-assisted procedure. The observed virions in the partially purified preparations were typical geminate particles with a size of ca. 18×30nm. This virus preparation was subjected to western blot analysis using antisera against Bean golden mosaic virus (BGMV) and Mungbean yellow mosaic virus (MYMV). SPLCV reacted with both antisera. Specific bands appeared to be slightly larger than the 30-kDa marker protein and were considered to be SPLCV coat protein. This western blot analysis revealed for the first time a serelogical relationship between SPLCV and the two well-characterized begomoviruses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00012942
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    Paper (German National Licenses)
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