ISSN:
1432-0614
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Abstract Xanthobacter autotrophicus GJ10 was applied in a packed-bed fermentor to degrade dichloroacetic acid (DCA) in batch-, semicontinuous and continuous culture. Degradation has been studied with freely suspended and adsorptive immobilized cells. To imitate natural soil systems, the fermentor was filled with sand. Concentrations of up to 20 mm DCA were degraded completely. If higher initial concentrations were used, the decrease in pH value inhibited further growth and degradation. In continuous culture the fermentor was inoculated additionally with activated sludge. Over a period of 2 weeks the specialized strain could be retained and no decrease in metabolic activity was observed. A decrease in degradation of DCA was observed when succinate was added as a second substrate. The haloacid dehalogenase was found to be induced by DCA. Non-induced cells showed typical repression of catabolites and diauxic growth with succinate as co-substrate. The results demonstrate that X. autotrophicus GJ10 might be suitable for applications in biological waste treatment systems.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00170446
