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  • 11
    ISSN: 1432-2048
    Keywords: Arabidopsis (mutants) ; Auxin ; Growth (root, auxin) ; Mutant (Arabidopsis) ; Root elongation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We modified a video digitizer system to allow short-term high-resolution measurements of root elongation in intact seedlings ofArabidopsis thaliana (L.) Heynh. We used the system to measure the kinetics of promotion and inhibition of root elongation by applied auxin and to determine the dose-response relationship for auxin action on elongation in roots of wild-type seedlings and seedlings of mutants (axr1,aux1, andaxr2) with altered auxin responsiveness. Roots of the mutants showed less inhibition in the presence of inhibitory concentrations of auxin than did roots of the wild type. The latent period preceding the change in elongation rate after auxin application was the same foraxr1 andaxr2 as for the wild type whereas the latent period foraux1 was about twice as long as for the wild type. Low concentrations (ca. 10−11 M) of auxin induced substantial promotion of root elongation in the wild type and inaxr2.
    Type of Medium: Electronic Resource
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  • 12
    ISSN: 1573-5087
    Keywords: Calmodulin ; gravitropism ; growth ; calcium ; auxin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract We examined the effect of calmodulin (CaM) antagonists applied at the root tip on root growth, gravity-induced root curvature, and the movement of calcium across the root tip and auxin (IAA) across the elongation zone of gravistimulated roots. All of the CaM antagonists used in these studies delayed gravity-induced curvature at a concentration (1 μM) that did not affect root growth. Calmodulin antagonists (≧ 1μM) inhibited downward transport of label from 45Ca2+ across the caps of gravistimulated roots relative to the downward transport of 45Ca2+ in gravistimulated roots which were not treated with CaM antagonists. Application of CaM antagonists at the root tip (≧ 1μM) also decreased the relative downward movement of label from 3H-IAA applied to the upper side of the elongation zone of gravistimulated roots. In general, tip application of antagonists inhibited neither the upward transport of 45Ca2+ in the root tip nor the upward movement of label from 3H-IAA in the elongation zone of gravistimulated roots. Thus, roots treated with CaM antagonists (≧ 1 μM) become less graviresponsive and exhibit reduced or even a reversal of downward polarity of calcium transport across the root tip and IAA transport across the elongation zone. The results indicate that calmodulin-regulated events play a role in root gravitropism.
    Type of Medium: Electronic Resource
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  • 13
    Electronic Resource
    Electronic Resource
    Springer
    Plant growth regulation 14 (1994), S. 235-242 
    ISSN: 1573-5087
    Keywords: auxin transport ; calcium ; gravitropism ; root cap ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract There is evidence that the cap is the initial site of lateral auxin redistribution during the gravitropic response of roots. We tested this further by comparing asymmetric auxin redistribution across the tips of gravistimulated intact roots, decapped roots, isolated root caps and isolated apical sections taken from decapped roots. Gravistimulation caused asymmetric (downward) auxin movement across the tips of intact roots and isolated root caps but not across the tips of decapped roots or across isolated apical root segments. Naphthylphthalamic acid and pyrenoylbenzoic acid, inhibitors of polar auxin transport, inhibited asymmetric auxin redistribution across gravistimulated isolated root caps and across the tips of gravistimulated intact roots. For intact roots there was a positive correlation between the extent of inhibition of assymmetric auxin redistribution by polar auxin transport inhibitors and the extent of inhibition of asymmetric calcium chelating agent, ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid, also caused parallel inhibition of asymmetric auxin redistribution and gravitropic curvature and this effect was reversed by subsequent treatment with calcium. The results support the hypothesis that the cap is a site of early development of auxin asymmetry in gravistimulated roots and that calcium plays an important role in the development of lateral auxin redistribution.
    Type of Medium: Electronic Resource
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  • 14
    ISSN: 1573-5079
    Keywords: oxygen evolving complex (OEC) ; EPR ; EXAFS
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Our recent EPR and EXAFS experiments investigating the structure of the oxygen-evolving complex of PS II are discussed. PS II treatments which affect the cofactors calcium and chloride have been used to poise samples in modified forms of the S-states, S1, S2 and S3. X-ray absorption studies indicate a similar overall structure for the manganese complex between treated and native samples although the influence of the treatments and cofactors is observed. Manganese oxidation (or oxidation of a ligand to the manganese cluster) is indicated to occur on each of the transitions S1 →S2 and S2 →S3 in these modified samples. The cluster appears to contain at least two inequivalent Mn-Mn pairs. In the native samples the Mn-Mn distance is 2.7 Å, but in samples where the calcium site is affected, one of the pairs has a 3.0 Å Mn-Mn distance. The intensity of the 3.3/3.6 Å interaction is reduced on sodium chloride treatment (calcium depletion) perhaps indicating calcium binding close to the manganese cluster. From EPR data we also propose that treatments which affect calcium and chloride binding cause a modification of the native S2 state, slow the reduction of Yz • and allow an S3 EPR signal to be observed following illumination. The origin of the S3 EPR signal, a modified S3 or S2 X• where X• is an organic radical of unknown charge, is discussed in relation to the results from the EXAFS studies.
    Type of Medium: Electronic Resource
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  • 15
    Electronic Resource
    Electronic Resource
    Springer
    Nonlinear dynamics 1 (1990), S. 91-116 
    ISSN: 1573-269X
    Keywords: internal resonance ; random vibrations ; non-Gaussian closure experiments
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract This paper presents the experimental results of random excitation of a nonlinear two-degree-of-freedom system in the neighborhood of internal resonance. The random signals of the excitation and response coordinates are processed to estimate the mean squares, autocorrelation functions, power spectral densities, and probability density functions. The results are qualitatively compared with those predicted by the Fokker-Planck equation together with a non-Gaussian closure scheme. The effects of system damping ratios, nonlinear coupling parameter, internal detuning ratio, and excitation spectral density level are considered in both studies except the effect of damping ratios is not considered in the experimental investigation. Both studies reveal similar dynamic features such as autoparametric absorber effect and stochastic instability of the coupled system. The experimental results show that the autocorrelation function of the coupled system has the feature of ultra narrow band process and degenerates to a periodic one as the internal detuning departs from the exact internal resonance condition. The measured probability density functions of the response of the main system suggests that the Gaussian representation is sufticient as long as the excitation level is relatively low in the neighborhood of the system internal resonance condition.
    Type of Medium: Electronic Resource
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  • 16
    Electronic Resource
    Electronic Resource
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 19 (1990), S. 13-26 
    ISSN: 1052-9306
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The determination of the amino acid composition and sequence of a peptide, using both conventional and tandem fast atom bombardment mass spectrometry, is presented. First a list of potential empirical formulae is generated using the accurate mass and isotopic peak intensity ratios from the molecular ion cluster. The formulae are mathematically analyzed to ensure that they correspond to linear peptides composed of 19 common amino acids. Then using the ions below m/z 160 Da, which are characteristic of the amino acid content of the peptide, the formulae are decomposed into possible amino acid compositions. From each composition sequences are generated and their predicted fragment ions are compared to ions present in the mass spectrum. A score based on the intensities and the occurrence of consecutive sequence fragments is calculated. Finally sequences with the ten highest scores are retained. Analyses performed on typical peptides with molecular weights below 1500 Da indicate that identification can generally be achieved using this approach.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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  • 17
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 16 (1990), S. 87-88 
    ISSN: 0741-0581
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
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