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  • 1
    Electronic Resource
    Electronic Resource
    Responses ofArabidopsis roots to auxin studied with high temporal resolution: Comparison of wild type and auxin-response mutants (1994)
    Springer
    Planta 194 (1994), S. 215-222 
    Details
    ISSN: 1432-2048
    Keywords: Arabidopsis (mutants) ; Auxin ; Growth (root, auxin) ; Mutant (Arabidopsis) ; Root elongation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We modified a video digitizer system to allow short-term high-resolution measurements of root elongation in intact seedlings ofArabidopsis thaliana (L.) Heynh. We used the system to measure the kinetics of promotion and inhibition of root elongation by applied auxin and to determine the dose-response relationship for auxin action on elongation in roots of wild-type seedlings and seedlings of mutants (axr1,aux1, andaxr2) with altered auxin responsiveness. Roots of the mutants showed less inhibition in the presence of inhibitory concentrations of auxin than did roots of the wild type. The latent period preceding the change in elongation rate after auxin application was the same foraxr1 andaxr2 as for the wild type whereas the latent period foraux1 was about twice as long as for the wild type. Low concentrations (ca. 10−11 M) of auxin induced substantial promotion of root elongation in the wild type and inaxr2.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01101680
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  • 2
    Electronic Resource
    Electronic Resource
    Responses of Arabidopsis roots to auxin studied with high temporal resolution: Comparison of wild type and auxin-response mutants (1994)
    Springer
    Planta 194 (1994), S. 215-222 
    Details
    ISSN: 1432-2048
    Keywords: Arabidopsis (mutants) ; Auxin ; Growth (root, auxin) ; Mutant (Arabidopsis) ; Root elongation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We modified a video digitizer system to allow short-term high-resolution measurements of root elongation in intact seedlings of Arabidopsis thaliana (L.) Heynh. We used the system to measure the kinetics of promotion and inhibition of root elongation by applied auxin and to determine the dose-response relationship for auxin action on elongation in roots of wild-type seedlings and seedlings of mutants (axr1, aux1, and axr2) with altered auxin responsiveness. Roots of the mutants showed less inhibition in the presence of inhibitory concentrations of auxin than did roots of the wild type. The latent period preceding the change in elongation rate after auxin application was the same for axr1 and axr2 as for the wild type whereas the latent period for aux1 was about twice as long as for the wild type. Low concentrations (ca. 10−11 M) of auxin induced substantial promotion of root elongation in the wild type and in axr2.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00196390
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    A highly repeated DNA sequence in Arabidopsis thaliana (1986)
    Springer
    Molecular genetics and genomics 204 (1986), S. 417-423 
    Details
    ISSN: 1617-4623
    Keywords: Repetitive DNA ; Arabidopsis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Three members of a family of highly repeated DNA sequences from Arabidopsis thaliana have been cloned and characterized. The repeat unit has an average length of 180 bp and is tandemly repeated in arrays longer than 50 kb. This family represents more than one percent of the Arabidopsis genome. Sequence comparisons with tandemly repeated DNA sequences from other Cruciferae species show several regions of homology and a similar length of the repeat unit. Homologies are also found to highly repeated sequences from other plant species. When the sequence CCGG occurs in the repeated DNA, the inner cytosine is generally methylated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00331018
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  • 4
    Electronic Resource
    Electronic Resource
    Genetic elements near the structural gene modulate the level of dopa decarboxylase during Drosophila development (1984)
    Springer
    Molecular genetics and genomics 195 (1984), S. 434-441 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Measurement of dopa decarboxylase (DDC) levels in 109 strains of Drosophila melanogaster isogenic for second chromosomes isolated independently from natural populations was undertaken. One of the most extreme variants, designated Ddc +4, was shown to have about 20% more DDC activity at adult eclosion than a standard laboratory strain used for comparison. The DDC overproduction was shown to segregate with the second chromosome and was mapped to a position within 0.15 map units of the DDC structural gene. The variant was shown to be an underproducer of DDC activity at pupariation and the genetic element responsible for this trait mapped in an identical fashion to that causing overproduction. The temporal phenotype described above was observed in the epidermis but DDC activity levels in neural tissue were normal. Examination of CRM levels at pupariation and eclosion revealed that altered DDC protein levels were responsible for the variant DDC activity levels. Electrophoretic analysis under both denaturing and non-denaturing conditions indicated that the DDC molecules in Ddc +4 and the laboratory strain were indistinguishable. These results suggest that alterations in a genetic element (or elements) lying in close proximity to the structural gene are responsible for the complex temporal phenotype of DDC activity exhibited in the variant Ddc +4.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00341444
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  • 5
    Electronic Resource
    Electronic Resource
    Insertion polymorphisms may cause stage specific variation in mRNA levels for dopa decarboxylase in Drosophila (1984)
    Springer
    Molecular genetics and genomics 195 (1984), S. 442-451 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary An activity variant in Drosophila, Ddc +4, which has been isolated from natural populations is shown to affect the level of messenger RNA for dopa decarboxylase (DDC) in a stage specific manner. Newly hatched first instar larvae and newly eclosed adults have 1.5 times the amount of DDC mRNA as the Canton-S laboratory strain. On the other hand, puparia of the variant have only 0.5 times as much DDC mRNA as Canton-S. Genomic Southern analysis revealed that Ddc +4 DNA differed from Canton-S DNA by four small restriction length polymorphisms. To confirm these differences, a library of Ddc +4 was constructed in the λ1059 vector. A clone was recovered spanning the DDC region and compared to cloned Canton-S DNA. Acrylamide gel electrophoresis of restriction fragments revealed that one previously identified insertion really consisted of two smaller ones. One of the other differences identified by the Southern analysis was not confirmed in the cloned DNA of Ddc +4, indicating some divergence had occurred in the variant strain between the time of its isogenization and cloning. The differences between the cloned Ddc +4 DNA and cloned Canton-S DNA consisted of six small restriction length polymorphisms and one restriction site polymorphism. Five of the seven differences lay in the 5′ untranslated leader sequence of the DDC mRNA or in the 4.5 kb of DNA upstream of the transcription start site. The existence of these small (〈100 bp) insertion/deletion polymorphisms in a strain exhibiting a complex temporal phenotype for DDC activity, suggests natural populations are an excellent source of variation affecting gene expression. Secondly, subtle restriction length polymorphisms near the 5′ end of genes may well be an important component of the variation upon which selection might be expected to act.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00341445
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  • 6
    Electronic Resource
    Electronic Resource
    Auxin-resistant mutants of Arabidopsis thaliana with an altered morphology (1987)
    Springer
    Molecular genetics and genomics 206 (1987), S. 200-206 
    Details
    ISSN: 1617-4623
    Keywords: Auxin resistance ; 2,4-Dichloro phenoxyacetic acid ; Phytohormone ; Plant development ; Herbicide-resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mutant lines of Arabidopsis thaliana resistant to the artificial auxin 2,4-dichloro phenoxyacetic acid (2,4-D) were isolated by screening for growth of seedlings in the presence of toxic levels of 2,4-D. Genetic analysis of these resistant lines indicated that 2,4-D resistance is due to a recessive mutation at a locus we have designated Axr-1. Mutant seedlings were resistant to approximately 50-fold higher concentrations of 2,4-D than wild-type and were also resistant to 8-fold higher concentrations of indole-3-acetic acid (IAA) than wild-type. Labelling studies with (14C)2,4-D suggest that resistance was not due to changes in uptake or metabolism of 2,4-D. In addition to auxin resistance the mutants have a distinct morphological phenotype including alterations of the roots, leaves, and flowers. Genetic evidence indicates that both auxin resistance and the morphological changes are due to the same mutation. Because of the pleiotropic morphological effects of these mutations the Axr-1 gene may code for a function involved in auxin action in all tissues of the plant.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00333575
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