Beschreibung: The brown shrimp Crangon crangon is a key component of the North Atlantic coastal food web and an important target species for the fishery economy. As the brown shrimp contains large amounts of protein and essential fatty acids, its consumption makes it a beneficial choice for humans. Commercially harvested crustaceans like C. crangon are frequently affected by bacterial shell disease, with necrotizing erosions and ulcerations of the cuticle. To determine whether shell disease influences the nutritional value of C. crangon, total protein and lipid contents, as well as fatty acid compositions of muscle tissue and hepatopancreas, together with the hepatosomatic index, were examined in healthy and affected individuals. The biochemical composition of the tissues did not differ significantly between the 2 groups. Also, the hepatosomatic index, as an indicator of energy reserves in shrimps, was similar between healthy and affected animals. Our results indicate that the nutritional value of C. crangon is not affected by shell disease, as long as it remains superficial as in the present study.

Beschreibung: The brown shrimp, Crangon crangon, is well adapted to the variable environmental conditions in the southern North Sea. It is very abundant, has high reproduction rates, and holds a key position in coastal ecosystems. This species has very low lipid deposits in the midgut gland, suggesting that the main function of the midgut gland is metabolic turnover rather than energy storage. Based on seasonal gene expression studies and established transcriptome data, we investigated key components of lipid metabolic pathways. Gene expression of triacylglycerol lipase, phospholipase, and fatty acid desaturase were analyzed and compared with that of other digestive enzymes involved in lipid, carbohydrate, and protein catabolism. Our results suggest that gene expression of digestive enzymes involved in lipid metabolism is modulated by the lipid content in the midgut gland and is related to food availability. Brown shrimp seem to be capable of using cellular phospholipids during periods of food paucity but high energetic (lipid) requirements. Two of three isoforms of fatty acid binding proteins (FABPs) from the midgut gland involved in fatty acid transport showed specific mutations of the binding site. We hypothesize that the mutations in FABPs and deficiencies in anabolic pathways limit lipid storage capacities in the midgut gland of C. crangon. In turn, food utilization, including lipid catabolism, has to be efficient to fulfill the energetic requirements of brown shrimp.

Beschreibung: Background: Trophic interactions are key processes, which determine the ecological function and performance of organisms. Many decapod crustaceans feed on plant material as a source for essential nutrients, e.g. polyunsaturated fatty acids. Strictly herbivorous feeding appears only occasionally in marine decapods but is common in land crabs. To verify food preferences and to establish trophic markers, we studied the lipid and fatty acid composition of the midgut glands of two marine crab species (Grapsus albolineatus and Percnon affine), one semi-terrestrial species (Orisarma intermedium, formerly Sesarmops intermedius), and one terrestrial species (Geothelphusa albogilva) from Taiwan. Results: All species showed a wide span of total lipid levels ranging from 4 to 42% of the dry mass (%DM) in the marine P. affine and from 3 to 25%DM in the terrestrial G. albogilva. Triacylglycerols (TAG) were the major storage lipid compound. The fatty acids 16:0, 18:1(n-9), and 20:4(n-6) prevailed in all species. Essential fatty acids such as 20:4(n-6) originated from the diet. Terrestrial species also showed relatively high amounts of 18:2(n-6), which is a trophic marker for vascular plants. The fatty acid compositions of the four species allow to clearly distinguish between marine and terrestrial herbivorous feeding due to significantly different amounts of 16:0, 18:1(n-9), and 18:2(n-6). Conclusions: Based on the fatty acid composition, marine/terrestrial herbivory indices were defined and compared with regard to their resolution and differentiating capacity. These indices can help to reveal trophic preferences of unexplored species, particularly in habitats of border regions like mangrove intertidal flats and estuaries.

Beschreibung: A pronounced seasonal variability in primary productivity is the crucial factor in determining the life strategies of many polar zooplankton species, whereas oligotrophic tropical oceans experience little change with a low productivity. Lipid accumulation represents an important energetic adaptation in pelagic organisms to cope with the pronounced seasonal productivity in polar oceans. We compare important global euphausiid species from the Arctic via the tropics to the Antarctic, focussing on the genera Euphausia and Thysanoessa. While the Antarctic krill Euphausia superba is the species with the highest biomass of all metazoans on earth, krill species are clearly less relevant in other oceans. In polar krill species total lipid accumulation is usually very pronounced and may be utilised for metabolic maintenance during overwintering or for reproductive processes in spring. Most polar krill species store large amounts of the unusual depot lipid phosphatidylcholine (lecithin), a polar lipid with highly unsaturated fatty acids. However, they exhibit strong differences in their neutral lipid compounds, which may either consist of wax esters or triacylglycerols, but also of both types of lipid classes. Their major end-products of the fatty acid and fatty alcohol biosynthesis are quite different and usually species-specific. In contrast, tropical euphausiids do not rely on lipids as energy reserves and exhibit the usual lipid and fatty acid compositions of biomembranes with high amounts of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA). In polar euphausiids, the major end-products of the fatty acid and fatty alcohol biosynthesis are quite different and usually species-specific. The ecophysiological implications of these deviating lipid characteristics may determine biogeographical zonation patterns and affect the vulnerability of polar krill species to global warming.

Beschreibung: Total lipids, lipid class and fatty acid compositions were studied to elucidate their role in the life cycle strategies of the Antarctic silverfish Pleuragramma antarcticum, one of the few truly pelagic fish species in the Antarctic realm. Various size groups (larvae to juveniles, 10-105 mm length) of this notothenioid fish have been collected in the southern Weddell Sea during different seasons (mainly spring: October/November and summer: January/February). Total lipid data of P. antarcticum revealed little variation between the seasons. Lipid levels were largely determined by the developmental stage and size, with lower lipid levels (〈20% of dry mass DM) found in the larvae (10-16 mm) and intermediate concentrations in the younger juveniles, which increased strongly to maximum amounts of lipid (〉40%DM) in the older juveniles (〉55-105 mm). Lipid class composition was clearly related to total lipid levels, with neutral lipids (triacylglycerols) comprising 〈20% of total lipid (%TL) in the younger lipid-poor specimens and 〉65%TL in the older lipid-rich fishes. Triacylglycerols and phospholipids showed an inverse relationship. Hence, the relative importance of these polar lipids, typically components of biomembranes, decreased with increasing lipid and triacylglycerol levels. Principal fatty acids were 14:0, 16:0, 18:1(n-9), 18:1(n-7), 20:5(n-3) and 22:6(n-3). Elevated portions of the long-chain mono¬unsaturated fatty acids, 20:1 and 22:1, in the lipid-rich specimens suggest the ingestion of two dominant calanoid copepod species, the wax ester-rich Calanoides acutus and the triacylglycerol-rich Calanus propinquus. They are the only species known to biosynthesize larger amounts of these monounsaturated compounds in high-Antarctic waters, which may therefore serve as dietary marker fatty acids for higher trophic levels. Apparently, wax ester moieties (fatty acids and alcohols) ingested with prey, e.g. copepods, are converted to triacylglycerols via fatty acids or metabolized by P. antarcticum. We discuss the function of these high-energy and low-density lipid compounds as energy reserve and/or buoyancy aid in this swimbladderless fish species.

Beschreibung: The aim of this study was to determine the macro-parasitic infestation level of oysters from the southern German Bight focussing on copepods of the genus Mytilicola. Crassostrea gigas, Ostrea edulis and Mytilus edulis were collected at five locations: three nearshore sites in the eastern Wadden Sea and two offshore cultivation sites in the German Bight. To reveal seasonal variations one sampling site was investigated in winter and summer. At the nearshore sites, Mytilicola orientalis was regularly detected in C. gigas. Prevalences ranged between 32.3% and 45.1%, intensity between 3.0 ± 0.6 and 8.2 ± 1.5. Infestation rates of C. gigas within the southern German Bight decreased from west to east: Apparently, M. orientalis has started its range extension along the German coast with gradual retardation eastwards but generally followed the invasion route of its main host, the Pacific oyster. Interestingly, we detected not only M. intestinalis but also M. orientalis as an intestinal parasite in M. edulis, which has sofar not previously been described as host within this region. We conclude that M. orientalis is flexible in its host choice. Furthermore, in the eastern Wadden Sea infestation rates of oysters and mussels by copepods are similar. These results deviate from the patterns observed for the northern Wadden Sea in terms of infestation level and host specificity. No macro-parasites were found in oysters and mussels from the offshore sites. This absence can be considered as potentially beneficial for aquaculture activities in the open ocean in terms of stamina and physiological performance.

Beschreibung: Antarctic and Arctic zooplankton species have developed very similar life strategies and energetic adaptations to the harsh environment via sophisticated modes of lipid accumulation. A very efficient biosynthesis, storage and utilization of lipids enable especially herbivorous species to buffer the pronounced seasonality of food supply in the polar oceans. Lipid levels usually peak at the end of the productive season in autumn and reach minimum levels in spring. In many species lipid deposits are not primarily used for maintenance during winter but are conserved to fuel reproductive processes at the end of the dark season. The dependence on seasonal primary production is also reflected by the respective lipid compositions. Detailed lipid analyses of dominant Antarctic and Arctic copepods revealed that the herbivorous Calanus and Calanoides species have developed the most complex lipid biochemical pathways. They biosynthesise large amounts of wax esters with long-chain monounsaturated fatty acids and alcohols (20:1, 22:1) as major components. In contrast, the Antarctic Calanus propinquus and C. simillimus synthesise primarily triacylglycerols consisting mainly of long-chain monounsaturated fatty acids with 22 and even 24 carbon atoms (2 major isomers), which is very unusual among plankton species. In contrast, the lipids of omnivorous and carnivorous taxa such as Metridia or Euchaeta are deficient in such long-chain fatty acids and alcohols, although their lipid reserves mainly consist of wax esters. Our investigations underscore that lipids are a key factor in high latitude ecosystems, especially for the lower trophic levels. The extremely lipid-rich herbivorous species ensure an efficient lipid-based energy transfer and represent high-calory food for fish and warm-blooded animals like birds and mammals.

Beschreibung: In the framework of the GENUS –“Geochemistry and Ecology of the Namibian Upwelling System” research program, trophic interactions and carbon pathways throughout the food web of the coastal upwelling system are being quantified. In contrast to earlier studies, special focus is been given to lower trophic levels in higher taxonomic resolution. Energy demands of various zooplankton taxa, including copepods, euphausiids, decapods and fish larvae, have been quantified with standardized methodology via optode respirometry. Dietary spectra and trophic levels were analyzed by trophic biomarker approaches based on fatty acid composition and stable isotopes (15N, 13C), respectively. All empirical data are assembled for an Ecopath with Ecosim (EwE) food-web model. The EwE model distinguishes between shelf and offshore communities. The conceptual food‐web model consists of three groups of primary producers, i.e. diatoms, dinoflagellates, and cyanobacteria, as well as many consumers such as Calanoides carinatus as the key herbivorous copepod in the Benguela upwelling system, other copepods, Euphausia hanseni, other zooplankton, and pelagic fishes including sardine, anchovy, and horse mackerel. Empirical data show that zooplankton and particularly copepods encompass a wider range of trophic levels from herbivory to secondary or even tertiary consumers (δ15N from 4 to 12‰), while anchovy had rather low δ15N of about 7‰. Respiration rates and metabolic activities of copepods could be parameterized for the model by an energy budget approach based on ambient temperature, body mass, and activity level. Calanoid copepods consumed 78mg C m‐2 d‐1 in shelf regions and 21mg C m‐2 d‐1 in oceanic regions. Locally, C. carinatus could remove up to 90% of the diatom biomass per day. The community consumption of pelagic decapods ranged from 7 mg C m‐2 d‐1 to 〉20mg C m‐2 d‐1 with highest values in the northernmost part of the study area. Overall, pelagic decapods apparently play a more prominent role in the northern Benguela ecosystem than previously assumed and may exert a substantial predation pressure on calanoid copepods. GENUS results emphasize that the trophic interactions within zooplankton and lower trophic levels are more complex than just linking primary producers with pelagic fish and should be taken into account in the process of developing realistic food‐web models of coastal upwelling systems. Keywords: foodweb, zooplankton, trophic interactions, energy flux