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  • 1
    Electronic Resource
    Electronic Resource
    Chlorophyll d as a major pigment (1996)
    [s.l.] : Nature Publishing Group
    Nature 383 (1996), S. 402-402 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] SIR á€" Chlorophyll d was reported first as a minor, green and magnesium-containing pigment in various species of red macroalgae in 1943 (ref. 1). It was thought by some, however, that chlorophyll d could be an artefact produced by the pigment extraction process, as it is not found ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/383402a0
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Rubisco activase transcript (rca) abundance increases when the marine unicellular green alga Chlorococcum littorale is grown under high-CO2 stress (1999)
    Springer
    Plant molecular biology 41 (1999), S. 627-635 
    Details
    ISSN: 1573-5028
    Keywords: green algae ; high-CO2 stress ; photosynthesis ; regulation ; Rubisco activase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract cDNA and the corresponding genomic DNA region encoding Rubisco activase were isolated from the unicellular green alga Chlorococcum littorale. The deduced amino acid sequence encoded by the cDNA was 403 amino acids long and exhibited important homology with those of other known Rubisco activases. Its N-terminal sequence was similar to the chloroplastic transit peptides in Chlamydomonas reinhardtii. The mature protein had a predicted molecular mass of 42 kDa. Five introns were located inside the genomic gene encoding Rubisco activase (rca). Genomic Southern blots indicated that two copies of the rca gene were present in the genome of C. littorale. The level of rca messenger RNA increased when cells of C. littorale were subjected to high-CO2 stress (i.e. grown under at least 20% CO2). Hsp70 heat-shock protein was also induced under high-CO2 conditions and, as expected, was also induced at 35 °C. The rca gene, in contrast, was not induced at 35 °C, indicating that this gene was induced in response to the high CO2 concentration and not to general stress. A search of the promoter-binding proteins by a gel retardation assay showed that, under the high-CO2 conditions, a protein(s) which was probably an activator of the rca transcription was synthesized.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006371905597
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Utilization and stability of vitamins in serum-containing and serum-free media in CHO cell culture (1990)
    Springer
    Cytotechnology 4 (1990), S. 243-250 
    Details
    ISSN: 1573-0778
    Keywords: Chinese hamster ovary (CHO) cells ; vitamins ; ascorbic acid ; choline ; nicotinamide ; thiamine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The concentrations of four vitamins, ascorbic acid, nicotinamide, choline and thiamine were evaluated in the culture supernatant of Chinese hamster ovary (CHO) cells. The media used were α-modified Eagle's minimum essential medium (MEM-α) supplemented with 10% fetal calf serum, and a 1:1 mixture of Ham's F12 and Dulbecco's modified Eagle's medium (DME/F12), containing neither serum nor protein. The reference experiment without cells revealed instability of ascorbic acid and thiamine. Moreover, a significant amount of each vitamin decreased in the culture supernatant. The possibility of growth limitation by vitamin depletion is strongly suggested.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00563784
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