ISSN:
1617-4623
Keywords:
Plasmid
;
Replication protein
;
Replication origin
;
Bacillus subtilis host
;
Bacillus amyloliquefaciens plasmid
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary The structure of a 1.5-kb DNA sequence that is necessary and sufficient for the replication of an 8.2-kb cryptic plasmid, pFTB14, isolated from a strain of Bacillus amyloliquefaciens has been characterized. The 1.5-kb DNA sequence contains an open reading frame, rep, stretching for 1017 bp, a promoter region for rep expression, and a possible replication origin for the plasmid upstream of the promoter. The rep product is trans-active and essential for plasmid replication. The predicted rep protein is a basic protein, as are the RepC protein of pT181, RepB of pUB110 and protein A of pC194 (all these found in staphylococci) and the π protein of the R6K plasmid of Escherichia coli. The predicted rep protein has highly homologous amino acid sequences with protein A of pC194 and RepC of pUB110 throughout the protein molecule, but not with RepC of pT181, π of R6K or protein RepH encoded by and iniating the replication of pC194.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00337763
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