Summary
The structure of a 1.5-kb DNA sequence that is necessary and sufficient for the replication of an 8.2-kb cryptic plasmid, pFTB14, isolated from a strain of Bacillus amyloliquefaciens has been characterized. The 1.5-kb DNA sequence contains an open reading frame, rep, stretching for 1017 bp, a promoter region for rep expression, and a possible replication origin for the plasmid upstream of the promoter. The rep product is trans-active and essential for plasmid replication. The predicted rep protein is a basic protein, as are the RepC protein of pT181, RepB of pUB110 and protein A of pC194 (all these found in staphylococci) and the π protein of the R6K plasmid of Escherichia coli. The predicted rep protein has highly homologous amino acid sequences with protein A of pC194 and RepC of pUB110 throughout the protein molecule, but not with RepC of pT181, π of R6K or protein RepH encoded by and iniating the replication of pC194.
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Communicated by M. Takanami
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Murai, M., Miyashita, H., Araki, H. et al. Molecular structure of the replication origin of a Bacillus amyloliquefaciens plasmid pFTB14. Mol Gen Genet 210, 92–100 (1987). https://doi.org/10.1007/BF00337763
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DOI: https://doi.org/10.1007/BF00337763