Publication Date:
2022-05-25
Description:
Author Posting. © The Author(s), 2011. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Journal of Insect Physiology 57 (2011): 665-675, doi:10.1016/j.jinsphys.2011.03.007.
Description:
Calanoid copepods, such as Calanus finmarchicus, are a key component of marine food webs. C.
finmarchicus undergoes a facultative diapause during juvenile development, which profoundly
affects their seasonal distribution and availability to their predators. The current ignorance of
how copepod diapause is regulated limits understanding of copepod population dynamics,
distribution, and ecosystem interactions. Heat shock proteins (Hsps) are a superfamily of
molecular chaperones characteristically upregulated in response to stress conditions and
frequently associated with diapause in other taxa. In this study, 8 heat shock proteins were
identified in C. finmarchicus C5 copepodids (Hsp21, Hsp22, p26, Hsp90, and 4 forms of Hsp70),
and expression of these transcripts was characterized in response to handling stress and in
association with diapause. Hsp21, Hsp22, and Hsp70A (cytosolic subfamily) were induced by
handling stress. Expression of Hsp70A was also elevated in shallow active copepodids relative to
deep diapausing copepodids, which may reflect induction of this gene by varied stressors in
active animals. In contrast, expression of Hsp22 was elevated in deep diapausing animals; Hsp22
may play a role both in short-term stress responses and in protecting proteins from degradation
during diapause. Expression of most of the Hsps examined did not vary in response to diapause,
perhaps because the diapause of C. finmarchicus is not associated with the extreme
environmental conditions (e.g., freezing, desiccation) experienced by many other taxa, such as
overwintering insects or Artemia cysts.
Description:
Funding for AMA was provided by the
WHOI Summer Student Fellowship Program and an EPA STAR fellowship.
Keywords:
Copepod
;
Crustacean
;
Diapause
;
Heat shock protein
;
Stress response
Repository Name:
Woods Hole Open Access Server
Type:
Preprint
Format:
application/pdf
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