ISSN:
1432-072X
Schlagwort(e):
Lenzites trabea
;
Basidiomycete
;
Submerged culture
;
Cellulase Production
;
β-glucosidase
;
Endo-1,4-β-glucanase
;
Enzyme purification
;
Carboxymethyl cellulose
;
Xylan
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
Notizen:
Abstract An extracellular endo-1,4-β-glucanase (EC 3.2.1.4) has been isolated and purified from the culture solution of the basidiomyceteLenzites trabea grown on glucose and cellulose. Besidesβ-glucosidase activity (EC 3.2.1.21) no evidence for C1-activity (EC 3.2.1.91) in the culture solution was found. The endoglucanase has been purified in a four-step procedure including chromatography on Sepharose 6-B and DEAE-Sephadex A-50, adsorption on hydroxylapatite and gel filtration on Bio-Gel P-100. The enzyme showed maximum activity at pH 4.4 and 70°C. A molecular weight of 29000 Daltons was estimated by calibration on Bio-Gel P-100. The enzyme hydrolyses carboxymethyl cellulose (CMC) as well as xylan.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00738548
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