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  • 1
    Online-Ressource
    Online-Ressource
    Saarbrücken : Institut für Sozialforschung und Sozialwirtschaft (iso) e.V.
    Schlagwort(e): Forschungsbericht ; Gesundheitswesen ; Nervendegeneration ; Krankenpflege ; Hauspflege ; Sekundärkrankheit ; Ambulanter Hilfs- und Pflegedienst ; Pflegeperson ; App ; Case Management ; Portal ; Prozessorganisation
    Materialart: Online-Ressource
    Seiten: 1 Online-Ressource (132 Seiten, 4,37 MB) , Illustrationen
    Serie: iso-Report Nr. 7
    Sprache: Deutsch
    Anmerkung: Förderkennzeichen BMBF 02L18A130-135 , Verbundnummer 01212224 , Laufzeit: 01.04.2020 bis 31.03.2023 , Das Vorhaben „ProDigA - Digital unterstützte Interaktionsarbeit“ (FKZ: 02L18A130) wurde im Rahmen des Programms „Zukunft der Arbeit“ vom Bundesministerium für Bildung und Forschung (BMBF) und dem Europäischen Sozialfonds (ESF) gefördert , Literaturangaben
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    Molecular genetics and genomics 205 (1986), S. 501-506 
    ISSN: 1617-4623
    Schlagwort(e): Signal sequence ; Antigenic epitopes ; Outer membrane protein ; Immunogenicity
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Fusion proteins comprising the amino-terminal 99 amino acids of the bacteriophage MS2 replicase and various portions of OmpV a major outer membrane protein of Vibrio cholerae were expressed in Escherichia coli K12. These fusions were expressed under the control of the PL promoter of bacteriophage λ, and expression was controlled using a cIts repressor. Fusions occurring within the secretory signal sequence of OmpV gave rise to the production of mature OmpV. The efficiency, however, decreased with progressive deletion of the signal sequence within the fusions. The reactivity of various OmpV fusions with antisera raised against purified OmpV and whole bacteria demonstrated the existence of two antigenic domains: one present in the denatured form and another in the membrane-associated form of OmpV. These domains correspond to markedly hydrophilic regions of the protein as would be predicted for surface-exposed epitopes.
    Materialart: Digitale Medien
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 3
    ISSN: 1573-0603
    Schlagwort(e): Antrum ; Human gastrointestinal epithelium ; Polarized epithelial cells ; Spheroid-like vesicles ; Tissue culture
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A novel procedure is described for the three-dimensional (3-D) in vitro culture and for maintaining of nontransformed gastric epithelial cells from the human antrum mucosa (HAEC). Biopsies obtained from the antrum were cut into small pieces and the tissue fragments were incubated in culture medium containing the appropriate antibiotics. The suspended mucosal fragments generated small, spheroid-like vesicles consisting of predominantly highly prismatic, mucus-producing cells which mimic the in vivo counterparts structurally and functionally. Electron microscopic investigations revealed a number of ultrastructural and morphological features similar to those of normal gastric cells in vivo such as apical microvilli associated with a glycocalyx, tight junctions, desmosomes, membraneous infoldings, mucous droplets, and an irregular basal lamina. In comparison to the two-dimensional (2-D) gastric cell cultures grown on plane supports, the vesicles maintain an intact epithelial organization of individual cells. The prismatic phenotype, the histophysiology as well as the cytoarchitecture of the non-transformed 3-D cultured gastric epithelial cells are comparable to those of the native tissue and therefore represent a suitable model for defined pathogen-host cell interactions.
    Materialart: Digitale Medien
    Standort Signatur Einschränkungen Verfügbarkeit
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