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  • 1
    ISSN: 1471-4159
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Medizin
    Notizen: Abstract: A 20-kDa DNA-binding protein that binds the AT-rich sequences within the promoters of the brain-specific protein kinase C (PKC) γ and neurogranin/RC3 genes has been characterized as chromosomal nonhistone high-mobility-group protein (HMG)-I. This protein is a substrate of PKC α, β, γ, and δ but is poorly phosphorylated by PKC ε and ζ. Two major (Ser44 and Ser64) and four minor phosphorylation sites have been identified. The extents of phosphorylation of Ser44 and Ser64 were 1:1, whereas those of the four minor sites all together were 〈30% of the major one. These PKC phosphorylation sites are distinct from those phosphorylated by cdc2 kinase, which phosphorylates Thr53 and Thr78. Phosphorylation of HMG-I by PKC resulted in a reduction of DNA-binding affinity by 28-fold as compared with 12-fold caused by the phosphorylation with cdc2 kinase. HMG-I could be additively phosphorylated by cdc2 kinase and PKC, and the resulting doubly phosphorylated protein exhibited a 〉 100-fold reduction in binding affinity. The two cdc2 kinase phosphorylation sites of HMG-I are adjacent to the N terminus of two of the three predicted DNA-binding domains. In comparison, one of the major PKC phosphorylation sites, Ser64, is adjacent to the C terminus of the second DNA-binding domain, whereas Ser44 is located within the spanning region between the first and second DNA-binding domains. The current results suggest that phosphorylation of the mammalian HMG-I by PKC alone or in combination with cdc2 kinase provides an effective mechanism for the regulation of HMG-I function.
    Materialart: Digitale Medien
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 2
    Publikationsdatum: 2013-08-31
    Beschreibung: [1]  One of the primary goals for the Visible Infrared Imaging Radiometer Suite (VIIRS) onboard the Suomi National Polar-orbiting Partnership (SNPP) is to provide the science and user communities with the data continuity of the Environmental Data Records (EDR) (or Level-2 products) over global oceanic waters for various research and applications, including assessment of climatic and environmental variations. The ocean color EDR is one of the most important products derived from VIIRS. Since ocean color EDR is processed from the upstream Sensor Data Records (SDR) (or Level-1B data), the objective of this study is to evaluate the impact of the SDR on the VIIRS ocean color EDR. The quality of the SDR relies on pre-launch sensor characterizations as well as on-orbit radiometric calibrations, which are used to develop the sensor F-factor lookup tables (F-LUTs). VIIRS F-LUTs derived from solar and lunar calibrations have been used in processing data from the VIIRS Raw Data Records (RDR) (or Level-0 data) to SDR. In this study, three sets of F-LUTs with different generation schemes have been used to reprocess the SDR and then the ocean color EDR for product evaluations. VIIRS ocean color products are compared with in situ data from the Marine Optical Buoy (MOBY) and products from the Moderate Resolution Imaging Spectroradiometer (MODIS) on the satellite Aqua. It is found that the data quality of VIIRS operational ocean color products before 6 February 2012 is poor due to the inappropriate use of the at-launch F-LUTs for the SDR calibration, and that the recently updated VIIRS F-LUTs have significantly improved the SDR and ocean color EDR. Using reprocessed SDR with updated F-LUTs and including vicarious calibration, VIIRS ocean color EDR products are consistent with those from MODIS-Aqua in global deep waters. Although there are still some significant issues with VIIRS ocean color EDR, e.g., poor data quality over coastal regions, our results demonstrate that VIIRS has great potential to provide the science and user communities with consistently high quality global ocean color data records that are established from heritage ocean color sensors such as MODIS-Aqua.
    Print ISSN: 0148-0227
    Thema: Geologie und Paläontologie , Physik
    Publiziert von Wiley-Blackwell im Namen von American Geophysical Union (AGU).
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 3
    Publikationsdatum: 2017-01-11
    Beschreibung: Unusual cold filaments are uncovered during the spring intermonsoon season in the South China Sea (SCS) using a suite of satellite observations. They have a width of about 100 km on average and extend several hundreds of kilometers offshore on the sea surface, providing significant cross-shelf transport of heat and nutrients. The eastward current associated with mesoscale eddies in spring in the western SCS is found to play an important role in the filament formation by advecting coastal cold waters far offshore. The meridional location of the cold filament displays considerable interannual variability ranging between 9 o N and 18 o N, which can be attributed to the interannual south-north shift of the eastward current associated with eddies. It is also found that in the spring, cold filaments have profound effects on the chlorophyll a concentration in the upper ocean and the overlying atmosphere. These findings provide new insights into the role of eddies in cross-shelf exchange and mesoscale air-sea interaction in the marginal seas.
    Print ISSN: 0148-0227
    Thema: Geologie und Paläontologie , Physik
    Publiziert von Wiley-Blackwell im Namen von American Geophysical Union (AGU).
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 4
    Publikationsdatum: 2018-05-15
    Beschreibung: Energies, Vol. 11, Pages 1242: Optimal Scheduling Strategies of Distributed Energy Storage Aggregator in Energy and Reserve Markets Considering Wind Power Uncertainties Energies doi: 10.3390/en11051242 Authors: Zengqiang Mi Yulong Jia Junjie Wang Xiaoming Zheng With continuous technological improvement and economic development of energy storage, distributed energy storage (DES) will be widely connected to the distribution network. If fragmented DES systems are aggregated to form a distributed energy storage aggregator (DESA), the DESA will have great potential to participate in the day-ahead energy and reserve market and the balancing market. The DESA could act as a mediator between the market and DES consumers, enabling beneficial coordination for DES owners and power systems. This paper presents a bilevel optimization model for DESAs in the energy and reserve market under wind power uncertainties. In the lower-level problem, generating companies, wind power plants (WPP), and DESAs are optimized for scheduling day-ahead (DA) energy and the reserve market. In the upper-level problem, operational strategies for DES systems and DESAs are designed to deal with wind power uncertainties in the balancing market. The DESA splits its resources between the energy and reserve markets so that it can reduce total power system consumption, and mutual profit for the system and end customers is achieved. This model is formulated as a mixed-integer linear programming (MILP) program, which can be solved with commercial software. The validity of the bilevel optimization model is verified by the eight-node test transmission system and IEEE-33 bus distribution system.
    Digitale ISSN: 1996-1073
    Thema: Energietechnik
    Publiziert von MDPI Publishing
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 5
    Publikationsdatum: 2014-08-13
    Beschreibung: P-n junction BiOBr/ZnO composites were prepared by a facile solvothermal process with double Br sources of CTAB and KBr. The samples were characterized by XRD, XPS, SEM, TEM, HRTEM, DRS, BET and PL. The BiOBr/ZnO composites exhibited much higher photocatalytic activity than single BiOBr and ZnO for the degradation of phenol under simulated sunlight irradiation. The enhanced photocatalytic activity of BiOBr/ZnO composites could be mainly ascribed to the high-efficiency separation of photogenerated electron-hole pairs through BiOBr/ZnO p-n junction. The reaction mechanism for the removal of phenol was also discussed. Hole and ·OH were the main reactive species. Moreover, the influence of disparate ratios of double Br sources to BiOBr/ZnO composites was also investigated. The results indicated that the BiOBr/ZnO composites prepared by double Br sources showed better photocatalytic activities than the sample prepared by single Br source. P-n junction BiOBr/ZnO composites were prepared by a facile solvothermal process with double Br sources and the composites exhibited higher photocatalytic activity than BiOBr and ZnO for the degradation of phenol, which could be ascribed to two reasons. One reason may be the high efficient separation of photogenerated electron-hole pairs through BiOBr/ZnO p-n junction. Another one would be the high surface area achieved by adjusting the content of CTAB.
    Print ISSN: 0232-1300
    Digitale ISSN: 1521-4079
    Thema: Geologie und Paläontologie , Physik
    Publiziert von Wiley-Blackwell
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 6
    Publikationsdatum: 2018-03-09
    Beschreibung: IJGI, Vol. 7, Pages 85: Assessment of Multiple GNSS Real-Time SSR Products from Different Analysis Centers ISPRS International Journal of Geo-Information doi: 10.3390/ijgi7030085 Authors: Zhiyu Wang Zishen Li Liang Wang Xiaoming Wang Hong Yuan The real-time State Space Representation (SSR) product of the GNSS (Global Navigation Satellite System) orbit and clock is one of the most essential corrections for real-time precise point positioning (PPP). In this work, the performance of current SSR products from eight analysis centers were assessed by comparing it with the final product and the accuracy of real-time PPP. Numerical results showed that (1) the accuracies of the GPS SSR product were better than 8 cm for the satellite orbit and 0.3 ns for the satellite clock; (2) the accuracies of the GLONASS (GLObalnaya NAvigatsionnaya Sputnikovaya Sistema) SSR product were better than 10 cm for orbit RMS (Root Mean Square) and 0.6 ns for clock STD (Standard Deviation); and (3) the accuracies of the BDS (BeiDou Navigation Satellite System) and Galileo SSR products from CLK93 were about 14.54 and 4.42 cm for the orbit RMS and 0.32 and 0.18 ns for the clock STD, respectively. The simulated kinematic PPP results obtained using the SSR products from CLK93 and CLK51 performed better than those using other SSR products; and the accuracy of PPP based on all products was better than 6 and 10 cm in the horizontal and vertical directions, respectively. The real-time kinematic PPP experiment carried out in Beijing, Tianjin, and Shijiazhuang, China indicated that the SSR product CLK93 from Centre National d’Etudes Spatiales (CNES) had a better performance than CAS01. Moreover, the PPP with GPS + BDS dual systems had a higher accuracy than those with only a GPS single system.
    Digitale ISSN: 2220-9964
    Thema: Architektur, Bauingenieurwesen, Vermessung , Geologie und Paläontologie
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 7
    Publikationsdatum: 2018-02-16
    Beschreibung: Energies, Vol. 11, Pages 438: Power-Balancing Based Induction Machine Model for Power System Dynamic Analysis in Electromechanical Timescale Energies doi: 10.3390/en11020438 Authors: Ding Wang Xiaoming Yuan Meiqing Zhang Power balance, including active and reactive power, between the system supply and the demand from induction motor loads is a potentially necessary condition for system stable operation. Motion of system states depends on the balancing of active and reactive powers. Therefore, this paper proposes an induction machine model in electromechanical timescale from a power balancing viewpoint, in which the induction motor load is modeled as a voltage vector driven by power balancing between the system supply and the demand from induction motor load, so as to describe the dynamic characteristics of induction motor loads in a physical way for power system dynamic analysis. Then a voltage magnitude-phase dynamic analysis with the proposed induction machine model is constructed. Based on the voltage magnitude-phase dynamic analysis, the characteristics of grid-connected induction motor loads are explored, and the instability mechanisms of grid-connected induction motor loads induced by a large disturbance are discussed. It is shown that the dynamic behavior of grid-connected induction motor loads can be described as the dynamic process of the terminal voltage vector driven by coupled active and reactive power balancing in different timescales. In this way, the dynamic behavior of induction motor loads in terms of voltage magnitude-phase dynamics and its physical characteristics are clearly illustrated. Time-domain simulation results are presented to validate the above analyses.
    Digitale ISSN: 1996-1073
    Thema: Energietechnik
    Publiziert von MDPI Publishing
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 8
    Publikationsdatum: 2017-11-08
    Beschreibung: Water, Vol. 9, Pages 865: Cyanobacterial Nitrogen Fixation Influences the Nitrogen Removal Efficiency in a Constructed Wetland Water doi: 10.3390/w9110865 Authors: Xiaodong Zhang Xin Jia Liang Yan Jinzhi Wang Xiaoming Kang Lijuan Cui Nitrogen removal efficiency in constructed wetlands (CW) is influenced by multiple environmental factors. However, little is known about the role of cyanobacterial nitrogen fixation in affecting nitrogen removal efficiency. This study investigated how cyanobacterial nitrogen fixation affects the efficiency, at which a CW removes nitrogen from an associated artificial lake (AL) in Beijing. For this purpose, we measured cell densities of N-fixing and non-N-fixing cyanobacteria, the aquatic nitrogen fixation rate (RNfix), and the concentration of various nitrogen fractions over the growing season (April–November) of 2014 in both AL and CW. We found that the removal of particulate organic nitrogen (PON) contributed to >90% of the total nitrogen removal in the CW. The removal efficiency of PON was lower during August–October (55.45 ± 27.49%) than during April–July (68.86 ± 8.83%). Phytoplankton proliferation in summer, as one of the main sources of PON, may have exceeded the capacity of the CW and led to declines in PON removal efficiency. RNfix peaked in July–October (3–169 ng N·L−1·h−1) and was positively correlated with both PON concentration and the cell density of N-fixing Anabaena sp. over the growing season, suggesting that aquatic nitrogen fixation (primarily in the AL) may increase PON and thereby reduce the its removal efficiency in the CW.
    Digitale ISSN: 2073-4441
    Thema: Energietechnik
    Publiziert von MDPI Publishing
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 9
    Publikationsdatum: 2018-05-02
    Beschreibung: Sensors, Vol. 18, Pages 1385: Geometric Integration of Hybrid Correspondences for RGB-D Unidirectional Tracking Sensors doi: 10.3390/s18051385 Authors: Shengjun Tang Wu Chen Weixi Wang Xiaoming Li Walid Darwish Wenbin Li Zhengdong Huang Han Hu Renzhong Guo Traditionally, visual-based RGB-D SLAM systems only use correspondences with valid depth values for camera tracking, thus ignoring the regions without 3D information. Due to the strict limitation on measurement distance and view angle, such systems adopt only short-range constraints which may introduce larger drift errors during long-distance unidirectional tracking. In this paper, we propose a novel geometric integration method that makes use of both 2D and 3D correspondences for RGB-D tracking. Our method handles the problem by exploring visual features both when depth information is available and when it is unknown. The system comprises two parts: coarse pose tracking with 3D correspondences, and geometric integration with hybrid correspondences. First, the coarse pose tracking generates the initial camera pose using 3D correspondences with frame-by-frame registration. The initial camera poses are then used as inputs for the geometric integration model, along with 3D correspondences, 2D-3D correspondences and 2D correspondences identified from frame pairs. The initial 3D location of the correspondence is determined in two ways, from depth image and by using the initial poses to triangulate. The model improves the camera poses and decreases drift error during long-distance RGB-D tracking iteratively. Experiments were conducted using data sequences collected by commercial Structure Sensors. The results verify that the geometric integration of hybrid correspondences effectively decreases the drift error and improves mapping accuracy. Furthermore, the model enables a comparative and synergistic use of datasets, including both 2D and 3D features.
    Digitale ISSN: 1424-8220
    Thema: Chemie und Pharmazie , Elektrotechnik, Elektronik, Nachrichtentechnik
    Publiziert von MDPI Publishing
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 10
    Publikationsdatum: 2013-03-26
    Beschreibung: AmyP is a raw-starch-degrading α-amylase newly identified from a marine metagenome library. It shares low sequence similarity with characterized glycoside hydrolases and was classified into a new subfamily of GH13. In particular, it showed preferential degradation to raw rice starch. Full-length AmyP was cloned and overexpressed in Escherichia coli , then purified and crystallized in the presence of its substrate analogue β-cyclodextrin. X-ray diffraction data were collected to a resolution of 2.1 Å. The crystal belonged to space group P 2 1 2 1 2, with unit-cell parameters a = 129.824, b = 215.534, c = 79.699 Å, α = β = γ = 90°, and was estimated to contain two molecules in one asymmetric unit.
    Digitale ISSN: 1744-3091
    Thema: Biologie , Chemie und Pharmazie , Geologie und Paläontologie , Physik
    Standort Signatur Einschränkungen Verfügbarkeit
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