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  • Springer  (14)
  • American Institute of Physics (AIP)  (1)
  • 1
    Electronic Resource
    Electronic Resource
    The kinetic and internal energy of NO from the photodissociation of nitrobenzene (1994)
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 100 (1994), S. 1946-1952 
    Details
    ISSN: 1089-7690
    Source: AIP Digital Archive
    Topics: Physics , Chemistry and Pharmacology
    Notes: Laser induced fluorescence probing of the nitric oxide fragment determines the distribution of rotational and vibrational energies of NO produced in the 226 and 280 nm photolysis of nitrobenzene. Combining these results with kinetic energy measurements using vacuum ultraviolet photoionization to detect the fragment gives a detailed view of the energy release in the photolysis. Boltzmann distributions describe the rotational state populations at both photolysis wavelengths. The rotational temperature of NO from the 226 nm photolysis is (3700±350) K, corresponding to an average rotational energy of (0.32±0.03) eV, and that of NO from the 280 nm photolysis is (2400±200) K, corresponding to an average rotational energy of (0.20±0.03) eV. We observe no vibrationally excited NO and place an upper limit of 10% on the fraction of nitric oxide produced in any one vibrationally excited state. Two different limiting models, impulsive energy release and statistical energy redistribution, both correctly predict much more rotational than vibrational excitation, but neither completely describes the observed internal and kinetic energies. The impulsive model finds more NO rotational and translational energy, but much less phenoxy fragment internal energy than we observe. The statistical model does better for the NO rotation and phenoxy fragment internal energy, but underestimates the translational energy substantially. A combination of these two types of behavior provides a physical picture that qualitatively explains our observations. It is likely that statistical energy redistribution occurs during the approach to the transition state for isomerization of nitrobenzene to phenyl nitrite and impulsive energy release dominates during the subsequent rupture of the CO–NO bond.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.466547
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  • 2
    Electronic Resource
    Electronic Resource
    Molecular principles of antigenic variation in Neisseria gonorrhoeae (1987)
    Springer
    Antonie van Leeuwenhoek 53 (1987), S. 431-434 
    Details
    ISSN: 1572-9699
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The genome of Neisseria gonorrhoeae harbours many gene loci for the production of variant pili. Strain MS11 has two expression genes (pilE) with promoter and complete coding sequences. The remaining genes are silent (pilS) lacking the promoter and the conservative amino terminals coding sequences of pilin. The pilus genes consist of six variable minicassettes (mc's), that are flancked by strictly conserved sequences. Upon phase (P+ to P+) and antigenic (P+ to P−, or vice versa) transitions minicassettes from silent loci are transferred from silent pilus gene copies to the expression gene by gene conversion. P− variants resulting from such rearrangements still produce pilin mRNA as well as pilin, but only a few are found on the surface of those gonococci.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00415498
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  • 3
    Electronic Resource
    Electronic Resource
    A rapid luciferase transfection assay for transcription activation effects and stability control of estrogenic drugs in cell cultures (1994)
    Springer
    Journal of cancer research and clinical oncology 120 (1994), S. 359-364 
    Details
    ISSN: 1432-1335
    Keywords: Estrogen receptor ; Estrogenic plantinum(II) complex ; Luciferase ; Transfection ; Drug stability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A rapid assay system for measuring the potential of estrogenic drugs is introduced. Luciferase induction could be measured in estrogen-receptor-positive human MCF-7 breast cancer cells, which had been transfected with a novel luciferase reporter plasmid ERE luc. The minimal requirement was 1 h exposure to the inducing drug and 3.5 h of incubation after removal of the drug. The assay system was used to measure the stability of the drug diaqua-[1,2-bis (2,6-dichloro-4-hydroxyphenyl) ethylenediamine] platinum(II) sulfate, containing an estrogenic ligand and reactive platinum. Luciferase activity was observed only when the drug was in the culture medium and cells for short times, whereas the estrogenic ligand alone remained active. It is assumed that binding of the platinum moiety to macromolecular constituents of the culture or cells renders the drug inaccessible for binding to the estrogen receptor.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01247461
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  • 4
    Electronic Resource
    Electronic Resource
    A Monte Carlo model to produce baryons ine + e − annihilation (1982)
    Springer
    The European physical journal 12 (1982), S. 77-79 
    Details
    ISSN: 1434-6052
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract A simple model is described extending the Field-Feynman model to baryon production in quark fragmentation. The model predicts baryon baryon correlations within jets and in opposite jets produced in electron-positron annihilation. Existing data is well described by the model.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01475734
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  • 5
    Electronic Resource
    Electronic Resource
    Isolation and culturing of highly polarized primary epithelial cells from normal human stomach (antrum) as spheroid-like vesicles (1997)
    Springer
    Methods in cell science 19 (1997), S. 169-178 
    Details
    ISSN: 1573-0603
    Keywords: Antrum ; Human gastrointestinal epithelium ; Polarized epithelial cells ; Spheroid-like vesicles ; Tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A novel procedure is described for the three-dimensional (3-D) in vitro culture and for maintaining of nontransformed gastric epithelial cells from the human antrum mucosa (HAEC). Biopsies obtained from the antrum were cut into small pieces and the tissue fragments were incubated in culture medium containing the appropriate antibiotics. The suspended mucosal fragments generated small, spheroid-like vesicles consisting of predominantly highly prismatic, mucus-producing cells which mimic the in vivo counterparts structurally and functionally. Electron microscopic investigations revealed a number of ultrastructural and morphological features similar to those of normal gastric cells in vivo such as apical microvilli associated with a glycocalyx, tight junctions, desmosomes, membraneous infoldings, mucous droplets, and an irregular basal lamina. In comparison to the two-dimensional (2-D) gastric cell cultures grown on plane supports, the vesicles maintain an intact epithelial organization of individual cells. The prismatic phenotype, the histophysiology as well as the cytoarchitecture of the non-transformed 3-D cultured gastric epithelial cells are comparable to those of the native tissue and therefore represent a suitable model for defined pathogen-host cell interactions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009751913391
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  • 6
    Electronic Resource
    Electronic Resource
    Construction of hermes shuttle vectors: a versatile system useful for genetic complementation of transformable and non-transformableNeisseria mutants (1996)
    Springer
    Molecular genetics and genomics 250 (1996), S. 558-569 
    Details
    ISSN: 1617-4623
    Keywords: Plasmid vector ; Conjugation ; Generalized mutagenesis ; Homologous recombination ; Natural transformation competence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A versatile shuttle system has been developed for genetic complementation with cloned genes of transformable and non-transformableNeisseria mutants. By random insertion of a selectable marker into the conjugativeNeisseria plasmidptetM25.2, a site within this plasmid was identified that is compatible with plasmid replication and with conjugative transfer of plasmid. Regions flanking the permissive insertion site of ptetM25.2 were cloned inEscherichia coli and served as a basis for the construction of the Hermes vectors. Hermes vectors are composed of anE. coli replicon that does not support autonomous replication inNeisseria, e.g. ColE1, p15A, orori fd, fused with a shuttle consisting of a selectable marker and a multiple cloning site flanked by the integration region of ptetM25.2. Complementation of a non-transformableNeisseria strain involves a three-step process: (i) insertion of the desired gene into a Hermes vector; (ii) transformation of Hermes into aNeisseria strain containing ptetM25.2 to create a hybrid ptetM25.2 via gene replacement by the Hermes shuttle cassette; and (iii) conjugative transfer of the hybrid ptetM25.2 into the finalNeisseria recipient. Several applications for the genetic manipulation of pathogenicNeisseriae are described.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02174444
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  • 7
    Electronic Resource
    Electronic Resource
    Virulence functions and antigen variation in pathogenic Neisseriae (1988)
    Springer
    Antonie van Leeuwenhoek 54 (1988), S. 421-430 
    Details
    ISSN: 1572-9699
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00461860
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  • 8
    Electronic Resource
    Electronic Resource
    Structure-based design of potent CDK1 inhibitors derived from olomoucine (2000)
    Springer
    Journal of computer aided molecular design 14 (2000), S. 403-409 
    Details
    ISSN: 1573-4951
    Keywords: cyclin-dependent kinase ; CDK1 ; inhibitor ; olomoucine ; structure-based design
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Cyclin-dependent kinase 1 (CDK1), an enzyme participating in the regulation of the cell cycle, constitutes a possible target in the search for new antitumor agents. Starting from the purine derivative olomoucine and following a structure-based approach, potent inhibitors of this enzyme were rapidly identified. The molecular modeling aspects of this work are described.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008115004986
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  • 9
    Electronic Resource
    Electronic Resource
    Kinetics of isomerization for the proline helical forms of two oligoproline redox arrays by circular dichroic spectropolarimetry (1999)
    Springer
    International journal of peptide research and therapeutics 6 (1999), S. 61-69 
    Details
    ISSN: 1573-3904
    Keywords: biexponential kinetics ; proline helices ; substituted proline residues
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The kinetics of isomerization of the helical forms of three oligoprolines was determined by far-ultraviolet CD spectropolarimetry and kinetic analysis by singular value decomposition. ZRA (Pro3-X-Pro2-Y-Pro2-Z-Pro3) and ZRA2 (Pro7-X-Pro2-Y-Pro2-Z-Pro7) bear large redox-active substituents on proline residues X, Y, and Z, but P9 (Pro9) does not. All three peptides formed a stable proline-II helix in water. In acetonitrile, both ZRA2 and P9 were converted into a proline-I helical form but ZRA remained predominantly in the proline-II helical form. Evidently, in order to undergo substantial proline II→I isomerization, an oligoproline chain containing large substituents needs to have a segment of consecutive unsubstituted proline residues that is sufficiently long to form a stable proline helix. Biexponential kinetics (A→B, k1 = ∼3.3 × 10-4 s-1; B→C, k2 = ∼0.8 × 10-4 s-1) were observed for the proline II→I isomerization of ZRA2 and P9 in acetonitrile and for the proline I→II isomerization of ZRA2 in water, which provides evidence for the growth and decay of a major kinetic intermediate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008819511721
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  • 10
    Electronic Resource
    Electronic Resource
    Serological properties and processing in Escherichia coli K12 of OmpV fusion proteins of Vibrio cholerae (1986)
    Springer
    Molecular genetics and genomics 205 (1986), S. 501-506 
    Details
    ISSN: 1617-4623
    Keywords: Signal sequence ; Antigenic epitopes ; Outer membrane protein ; Immunogenicity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Fusion proteins comprising the amino-terminal 99 amino acids of the bacteriophage MS2 replicase and various portions of OmpV a major outer membrane protein of Vibrio cholerae were expressed in Escherichia coli K12. These fusions were expressed under the control of the PL promoter of bacteriophage λ, and expression was controlled using a cIts repressor. Fusions occurring within the secretory signal sequence of OmpV gave rise to the production of mature OmpV. The efficiency, however, decreased with progressive deletion of the signal sequence within the fusions. The reactivity of various OmpV fusions with antisera raised against purified OmpV and whole bacteria demonstrated the existence of two antigenic domains: one present in the denatured form and another in the membrane-associated form of OmpV. These domains correspond to markedly hydrophilic regions of the protein as would be predicted for surface-exposed epitopes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00338089
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