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  • Springer  (14)
  • 2000-2004  (14)
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  • 1
    ISSN: 1432-086X
    Keywords: Key words: Peripheral arterial occlusive disease—Intermittent claudication—Angioplasty—Superficial femoral artery, long-segment occlusion—Distal ostial collateral stenosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Purpose: To evaluate the angiographic and clinical effects of percutaneous transluminal angioplasty (PTA) of distal ostial collateral stenoses in patients with claudication and long-segment occlusion of the superficial femoral artery (SFA). Methods: In ten patients (9 men, 1 woman) with stable intermittent claudication due to chronic long-segment occlusion of the SFA a high-grade stenosis of the distal collateral ostium of the deep femoral artery to the popliteal artery were dilated. PTA was performed using popliteal artery access. Claudication distances on the treadmill and ankle-brachial pressure indices (ABI) at rest were analyzed before, 1 week, and 14 weeks after PTA. Results: Initial technical success was obtained in all patients. There were no significant periprocedural local complications. The initial mean claudication distance on the treadmill increased significantly from 107 ± 65 m to 306 ± 209 m (p 〈 0.01), the maximal claudication distance from 203 ± 128 m to 392 ± 167 m (p 〈 0.01). The mean ABI changed slightly but significantly (0.61 ± 0.08 vs. 0.64 ± 0.07; p 〈 0.05). Early follow-up after 14 weeks revealed no clinical deterioration. Conclusion: This new technique is considered helpful in patients with well-defined claudication and long-segment occlusion of the SFA.
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  • 2
    ISSN: 1432-0738
    Keywords: Key words NQO1 polymorphism ; Real-time PCR ; Colorectal cancer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The present study used a rapid and single-step method for genotyping of NAD(P)H quinone oxidoreductase (NQO1) codon 609 polymorphism using real-time polymerase chain reaction (PCR)-analysis and subsequent melting curve analysis for the analysis of allelic distribution of NQO1. The design was a case control study of 323 Caucasians with colorectal cancer and 205 healthy controls. There was no difference in the frequencies of the mutated NQO1 allele (NQO1*2): 0.190 for control individuals and 0.195 for cancer patients, respectively (P=0.947). When this allelic distribution was further compared between non-smoking and smoking colorectal cancer patients, it appeared that the frequency of the wild-type allele NQO1*1 was higher in the smoking than in the non-smoking group [Odds ratio (OR), 0.434; 95% confidence interval (CI), 0.13–1.42]. This observation may suggest a protective role of the NQO1 wild-type allele in colon cancer susceptibility of individuals exposed to NQO1-inducing chemicals.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-1084
    Keywords: Key words: Renal arteries – Stenosis or obstruction – Renal arteries – Transluminal angioplasty – Stents and prostheses – Renal hypertension
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. The aim of this study was to determine long-term success of flexible tantalum stents for the treatment of ostial and truncal renal artery stenosis. Since 1989, flexible tantalum stents (type Strecker) were implanted in 34 patients (36 arteries, 25 ostial lesions, 11 truncal lesions) with uncontrollable renovascular hypertension, 9 of them in association with renal insufficiency. Stents were placed unilaterally in 32 patients, and bilaterally in 2 patients for the treatment of renal artery stenosis. Thirty-five of 36 lesions were atherosclerotic, including 5 recurrencies after previous percutaneous transluminal renal angioplasty (PTRA). One patient had Takayasu arteritis. Stents were implanted after unsuccessful PTRA of 11 truncal and 23 ostial lesions, and as a primary procedure in 2 ostial lesions. Follow-up examinations included blood pressure measurement, determination of serum creatinine level, color duplex sonography, or angiography. The technical success rate was 92 %. Technical failure included incorrect stent placement (1 of 36 lesions, 2.8 %), and stent dislocation (2 of 36 lesions, 5.6 %), and two stents were retrieved percutaneously. In one case of Takayasu arteritis, residual stenosis of 40 % was observed. After technically successful stent placement, 77 % of patients became normotensive with or without medication. In the remaining patients there was partial improvement with blood pressure between 140 and 180 mmHg. Renal function improved in 76 % of patients (completely in 3 of 8, 38 %; and partially in 3 of 8, 38 %). Primary patency rate including all stented lesions and initial technical failures was 82.4 % ± 6.8 (1 year) and 82.4 % ± 9.2 (3 years). After technically successful stent placement, patency rates were 89.9 ± 5.6 % (1 year), and 89.9 ± 7.6 % (3 years). For ostial lesions, primary patency rate was 87.9 ± 6.7 % (1 year) and 87.9 ± 9.2 % (3 years). Placement of flexible tantalum stents in renal arteries is technically demanding, especially in ostial lesions. Once placed successfully, stent patency rate is excellent.
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  • 4
    ISSN: 1432-1238
    Keywords: Streptococcal toxic shock syndrome Capillary leak syndrome Necrotizing fasciitis C1-esterase inhibitor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Objective: To evaluate the effect of adjunctive C1-esterase inhibitor substitution therapy on clinical characteristics and outcome of patients with streptococcal toxic shock syndrome (TSS). Design: Observational. Setting: Medizinische Poliklinik, University of Bonn, Germany. Patients: Seven patients with direct or indirect evidence of streptococcal TSS. Intervention: In addition to conventional and supportive therapy, all patients received 2–3 single doses of C1-esterase inhibitor totaling 6,000–10,000 U within the first 24 h after admission. Measurements and results: All patients developed fulminant septic shock, multiorgan failure and/or capillary leak syndrome and necrotizing fasciitis within 10–72 h following the onset of first symptoms. Between 1 and 4 days following administration of C1-esterase inhibitor, a marked shift of fluid from extravascular to intravascular compartments took place in all but one patient, accompanied by a transient intra-alveolar lung edema and rapidly decreasing need for adrenergic agents. Six of seven patients survived. Conclusions: These clinical observations in a small series of patients and the favorable outcome point towards a positive effect of early and high-dose administration of C1-esterase inhibitor as adjunctive therapy in streptococcal TSS. The possible mechanism involved may be the attenuation of capillary leak syndrome (CLS) via early inactivation of complement and contact systems. Controlled studies are needed to establish an improvement of the survival rates of patients with streptococcal TSS following administration of C1-esterase inhibitor.
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  • 5
    ISSN: 1432-2013
    Keywords: β-Adrenergic stimulation Atria Calcium fluorescence Cardiac myocytes Cell contractility Phospholamban Relaxation SERCA2a
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Cardiac myocyte relaxation is brought about mainly through Ca2+ uptake into the sarcoplasmic reticulum (SR) by a Ca2+-ATPase isoform, SERCA2a. Its activity is modulated by another protein, phospholamban (PLB). The levels of both proteins differ in some mammals between atrial and ventricular myocardium and this may lead to differences in relaxation, especially under stimulatory conditions. At a concentration of 100 nM, the β-adrenergic agonist isoprenaline (ISO) accelerates the relaxation of rat papillary muscle more than that of the left atria (16.4 versus 4.0% hastening of time to 50% relaxation, respectively). Ventricular myocytes were 24.7% quicker in reaching 50% of their diastolic length after contraction when treated with ISO compared to atrial myocytes, which were only 3.6% faster. Ca2+ fluorescence transients were also abbreviated in ventricular compared to atrial myocytes exposed to ISO (41.9 versus 25.2% hastening of time to 50% peak Ca2+ respectively). Ca2+ uptake into ventricular SR vesicles was increased by 13% in the presence of protein kinase A while that into atrial SR vesicles remained unaffected. Western blotting analysis revealed 23% less SERCA2a protein, but 76% more PLB in ventricular compared to atrial tissue. We conclude that the distinct levels of SERCA2a and PLB in ventricular and atrial myocardium are responsible for the differential modulation of the relaxation process arising from β-adrenergic stimulation in single rat atrial and ventricular myocytes.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1434-601X
    Keywords: PACS. 21.60.-n Nuclear-structure models and methods – 23.20.Lv Gamma transitions and level energies – 27.80.+w 190 ≤A≤ 219
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract: High-spin states in 202Pb and 203Pb have been investigated by in-beam γ-ray spectroscopy following the reaction 198Pt(9Be,xn). A search for magnetic rotational bands in these isotopes confirmed one of the two bands previously assigned to 202Pb and revealed a new band in this isotope. No evidence for magnetic rotation has been found in 203Pb.
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  • 7
    ISSN: 1432-0568
    Keywords: Key words Blood vessel walls ; Intima thickening ; Lamina elastica interna splitting ; Morphology ; Elastin quantification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Although pre-eclampsia (PE) is often associated with fetal hypoxia, hypertension and/or disturbed function of the fetal circulation, the effect of these altered hemodynamic parameters on the structure and composition of umbilical vessels has not been systematically investigated before. Therefore, this study focuses on PE-associated changes of the elastic fibre system in umbilical cord vessels investigated by light and electron microscopy, immunocytochemistry and biochemistry. In umbilical cord veins, no changes in thickness of the vessel wall or of any sublayer were observed. However, the internal elastic lamina of the veins was split in 80% of the PE-group in contrast to 20% in uncomplicated pregnancies. This effect was significant (α 〈0.01) from 36 weeks of gestation onwards. In umbilical cord arteries, the entire arterial vessel wall was found to be 15% thicker in PE than in uncomplicated pregnancies. The enlargement was caused by an increase of both the tunica intima and tunica media. The thickening of the tunica intima was attributed to a migration of smooth muscle cells towards the endothelium, accompanied by a splitting of the internal elastic lamina. Compared to uncomplicated pregnancies, smooth muscle cells of arteries and veins in PE showed a metabolic activation demonstrated by highly dilated endoplasmic reticulum. A semiquantitative score method as well as a quantitative dot blot assay indicated a PE-associated reduction of elastin expression in the arterial vessel walls. In summary, PE obviously induces a decrease of the elastin content accompanied by a thickening of the vessel wall in umbilical cord arteries. This remodeling of the elastic fibre system, together with an increased migration of smooth muscle cells, might represent part of the functional adaptation system of the umbilical cord arteries on the altered hemodynamic conditions in PE.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    European food research and technology 211 (2000), S. 346-348 
    ISSN: 1438-2385
    Keywords: Key wordsCraterellus ; Gomphidius ; Higher fungi ; Hydnum ; Hygrophorus, Laccaria ; Trypsin inhibitors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Laccaria and Hygrophorus species, Craterellus cornucopioides, Gomphidius glutinosus, Macrolepiota rhacodes and Hydnum repandum. The inhibiting activities found were higher than those of cereals but lower than those of some fabaceous plants.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Annals of biomedical engineering 28 (2000), S. 781-792 
    ISSN: 1573-9686
    Keywords: Ventricular mechanics ; Scalable parallel computation ; Finite element analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Technology
    Notes: Abstract To determine regional stress and strain distributions in rabbit ventricular myocardium, an anatomically detailed finite element model was used to solve the equations of stress equilibrium during passive filling of the left ventricle. Computations were conducted on a scalable parallel processing computer and performance was found to scale well with the number of processors used, so that stimulations previously requiring approximately 60 min were completed in just over 5 min. Epicardial strains from the model analysis showed good agreement (RMSE=0.007332) with experimental measurements when material properties were chosen such that cross fiber strain was more heterogeneous than fiber strain, which is also consistent with experimental observations in other species. © 2000 Biomedical Engineering Society. PAC00: 8719Hh, 8719Rr, 8710+e
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Molecular diversity 5 (2000), S. 111-116 
    ISSN: 1573-501X
    Keywords: combinatorial chemistry ; delivery microreactors ; drop-on-demand liquid ; miniaturization ; photolysis ; silicon wafer ; single bead synthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Microstructured silicon wafers were employed as miniaturizedsolid-phase reaction vessels as well as miniaturized microtiter plates. Employing piezoelectric drop-on-demand liquidjets, a combinatorial library of 256 Peptides was synthesizedon single beads. The synthesis protocol was associated to thelocation in the silicon nano-well arrangement. Products werephotolytically cleaved in the same well that was used forsynthesis and subsequently interrogated for thrombin inhibitionin a homogeneous competition assay. The assay procedure wasbased on drop-on-demand liquid delivery and laser inducedfluorescence imaging. The novel format proved useful for theintegration of both synthesis and screening into one platform,a prerequisite for an iterative, evolutionary approach towardsdrug discovery.
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