Description: The voltage-gated sodium channel (VGSC) interacting peptide is of special interest for both basic research and pharmaceutical purposes. In this study, we established a yeast-two-hybrid based strategy to detect the interaction(s) between neurotoxic peptide and the extracellular region of VGSC. Using a previously reported neurotoxin JZTX-III as a model molecule, we demonstrated that the interactions between JZTX-III and the extracellular regions of its target hNav1.5 are detectable and the detected interactions are directly related to its activity. We further applied this strategy to the screening of VGSC interacting peptides. Using the extracellular region of hNav1.5 as the bait, we identified a novel sodium channel inhibitor SSCM-1 from a random peptide library. This peptide selectively inhibits hNav1.5 currents in the whole-cell patch clamp assays. This strategy might be used for the large scale screening for target-specific interacting peptides of VGSCs or other ion channels. Scientific Reports 4 doi: 10.1038/srep04569

Description: State-of-the-art pansharpening methods generally inject the spatial structures of a high spatial resolution (HR) panchromatic (PAN) image into the corresponding low spatial resolution (LR) multispectral (MS) image by an injection model. In this paper, a novel pansharpening method with an edge-preserving guided filter based on three-layer decomposition is proposed. In the proposed method, the PAN image is decomposed into three layers: A strong edge layer, a detail layer, and a low-frequency layer. The edge layer and detail layer are then injected into the MS image by a proportional injection model. In addition, two new quantitative evaluation indices, including the modified correlation coefficient (MCC) and the modified universal image quality index (MUIQI) are developed. The proposed method was tested and verified by IKONOS, QuickBird, and Gaofen (GF)-1 satellite images, and it was compared with several of state-of-the-art pansharpening methods from both qualitative and quantitative aspects. The experimental results confirm the superiority of the proposed method.

Description: The structural properties of the uranium-encapsulated nano-cage U@Au14 are predicted using density functional theory. The presence of the uranium atom makes the Au14 structure more stable than the empty Au14-cage, with a triplet ground electronic state for U@Au14. Analysis of the electronic structure shows that the two frontier single-occupied molecular orbital electrons of U@Au14 mainly originate from the 5f shell of the U atom after charge transfer. Meanwhile, the bonding orbitals and charge population indicate that the designed U@Au14 nano-cage structure is stabilized by ionocovalent interactions. The current findings provide theoretical basis for future syntheses and further study of actinide doped gold nanoclusters, which might subsequently facilitate applications of such structure in radio-labeling, nanodrug carrier and other biomedical applications. Scientific Reports 4 doi: 10.1038/srep05862

Description: Remote Sensing, Vol. 10, Pages 474: Evaluating Eight Global Reanalysis Products for Atmospheric Correction of Thermal Infrared Sensor—Application to Landsat 8 TIRS10 Data Remote Sensing doi: 10.3390/rs10030474 Authors: Xiangchen Meng Jie Cheng Global reanalysis products have been widely used for correcting the atmospheric effects of thermal infrared data, but their performances have not been comprehensively evaluated. In this paper, we evaluate eight global reanalysis products (NCEP/FNL; NCEP/DOE Reanalysis2; MERRA-3; MERRA-6; MERRA2-3; MERRA2-6; JRA-55; and ERA-Interim) commonly used in the atmospheric correction of Landsat 8 TIRS10 data by referencing global radiosonde observations collected from 163 stations. The atmospheric parameters (atmospheric transmittance, upward radiance, and downward radiance) simulated with MERRA-6 and ERA-Interim were accurate than those simulated with other reanalysis products for different water vapor contents and surface elevations. When global reanalysis products were applied to retrieve land surface temperature (LST) from simulated Landsat 8 TIRS10 data, ERA-Interim and MERRA-6 were accurate than other reanalysis products. The overall LST biases and RMSEs between the retrieved LSTs and LSTs that were used to generate the top-of-atmosphere radiances were less than 0.2 K and 1.09 K, respectively. When eight reanalysis products were used to estimate LSTs from thirty-two Landsat 8 TIRS10 images covering the Heihe River basin in China, the various reanalysis products showed similar validation accuracies for LSTs with low water vapor contents. The biases ranged from 0.07 K to 0.24 K, and the STDs (RMSEs) ranged from 1.93 K (1.93 K) to 2.02 K (2.04 K). Considering the above evaluation results, MERRA-6 and ERA-Interim are recommended for thermal infrared data atmospheric corrections.

Description: A film comprising randomly distributed metal/dielectric/metal sandwich nanopillars with a distribution of cross-sectional diameters, displayed extremely low reflectance over the blue-to-red regime, when coated on glass and illuminated normally. When it is illuminated by normally incident light, this sandwich film (SWF) has a low extinction coefficient, its phase thickness is close to a negative wavelength in the blue-to-red spectral regime, and it provides weakly dispersive forward and backward impedances, so that reflected waves from the two faces of the SWF interfere destructively. Broadband reflection-reduction, over a wide range of incidence angles and regardless of the polarization state of the incident light, was observed when the SWF was deposited on polished silicon. Scientific Reports 3 doi: 10.1038/srep01672

Description: Nucleic acid based molecular device is a developing research field which attracts great interests in material for building machinelike nanodevices. G-quadruplex, as a new type of DNA secondary structures, can be harnessed to construct molecular device owing to its rich structural polymorphism. Herein, we developed a switching system based on G-quadruplexes and methylazacalix[6]pyridine (MACP6). The induced circular dichroism (CD) signal of MACP6 was used to monitor the switch controlled by temperature or pH value. Furthermore, the CD titration, Job-plot, variable temperature CD and 1H-NMR experiments not only confirmed the binding mode between MACP6 and G-quadruplex, but also explained the difference switching effect of MACP6 and various G-quadruplexes. The established strategy has the potential to be used as the chiral probe for specific G-quadruplex recognition. Scientific Reports 5 doi: 10.1038/srep10479

Description: Iron overload, caused by hereditary hemochromatosis or repeated blood transfusions in some diseases, such as beta thalassemia, bone marrow failure and myelodysplastic syndrome, can significantly induce injured bone marrow (BM) function as well as parenchyma organ dysfunctions. However, the effect of iron overload and its mechanism remain elusive. In this study, we investigated the effects of iron overload on the hematopoietic stem and progenitor cells (HSPCs) from a mouse model. Our results showed that iron overload markedly decreased the ratio and clonogenic function of murine HSPCs by the elevation of reactive oxygen species (ROS). This finding is supported by the results of NAC or DFX treatment, which reduced ROS level by inhibiting NOX4 and p38MAPK and improved the long-term and multi-lineage engrafment of iron overload HSCs after transplantation. Therefore, all of these data demonstrate that iron overload injures the hematopoiesis of BM by enhancing ROS through NOX4 and p38MAPK. This will be helpful for the treatment of iron overload in patients with hematopoietic dysfunction. Scientific Reports 5 doi: 10.1038/srep10181

Description: Remote Sensing, Vol. 9, Pages 1247: Estimating Land Surface Temperature from Feng Yun-3C/MERSI Data Using a New Land Surface Emissivity Scheme Remote Sensing doi: 10.3390/rs9121247 Authors: Xiangchen Meng Jie Cheng Shunlin Liang Land surface temperature (LST) is a key parameter for a wide number of applications, including hydrology, meteorology and surface energy balance. In this study, we first proposed a new land surface emissivity (LSE) scheme, including a lookup table-based method to determine the vegetated surface emissivity and an empirical method to derive the bare soil emissivity from the Global LAnd Surface Satellite (GLASS) broadband emissivity (BBE) product. Then, the Modern Era Retrospective-Analysis for Research and Applications (MERRA) reanalysis data and the Feng Yun-3C/Medium Resolution Spectral Imager (FY-3C/MERSI) precipitable water vapor product were used to correct the atmospheric effects. After resolving the land surface emissivity and atmospheric effects, the LST was derived in a straightforward manner from the FY-3C/MERSI data by the radiative transfer equation algorithm and the generalized single-channel algorithm. The mean difference between the derived LSE and field-measured LSE over seven stations is approximately 0.002. Validation of the LST retrieved with the LSE determined by the new scheme can achieve an acceptable accuracy. The absolute biases are less than 1 K and the STDs (RMSEs) are less than 1.95 K (2.2 K) for both the 1000 m and 250 m spatial resolutions. The LST accuracy is superior to that retrieved with the LSE determined by the commonly used Normalized Difference Vegetation Index (NDVI) threshold method. Thus, the new emissivity scheme can be used to improve the accuracy of the LSE and further the LST for sensors with broad spectral ranges such as FY-3C/MERSI.

Description: Energies, Vol. 11, Pages 1632: Interleaved High Step-Up DC-DC Converter Based on Voltage Multiplier Cell and Voltage-Stacking Techniques for Renewable Energy Applications † Energies doi: 10.3390/en11071632 Authors: Shin-Ju Chen Sung-Pei Yang Chao-Ming Huang Huann-Ming Chou Meng-Jie Shen A novel interleaved high step-up DC-DC converter based on voltage multiplier cell and voltage-stacking techniques is proposed for the power conversion in renewable energy power systems. The circuit configuration incorporates an input-parallel output-series boost converter with coupled inductors, clamp circuits and a voltage multiplier cell stacking on the output side to extend the voltage gain. The converter achieves high voltage conversion ratio without working at extreme large duty ratio. The voltage stresses on the power switches are significantly lower than the output voltage. As a result, the low-voltage-rated metal-oxide-semiconductor field-effect transistors (MOSFETs) can be employed to reduce the conduction losses and higher conversion efficiency can be expected. The interleaved operation reduces the input current ripple. The leakage inductances of the coupled inductors act on mitigating the diode reverse recovery problem. The operating principle, steady-state analysis and design guidelines of the proposed converter are presented in detail. Finally, a 1-kW prototype with 28-V input and 380-V output voltages was implemented and tested. The experimental results are presented to validate the performance of the proposed converter.