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  • Institut für Meereskunde  (5)
  • 1
    Publication Date: 2022-05-04
    Description: The present paper describes an improved method for the semicontinuous culture of bacterial populations on Nuclepore membrane filters. The filters, placed on swimming rings, are incubated in direct contact with the water surface of the flow system, and examined by fluorescence microscopy. The growth activity of both, pure cultures and natural bacterial populations, measured in the flow system is compared to the activity measured in a conventional culture system (incubation of membrane filters on the surface of paper disc saturated with incubation medium). The flow system increases the number and the spectrum of colony forming cells. The reasons for the superiority of the flow system are discussed.
    Type: Article , NonPeerReviewed
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  • 2
    Publication Date: 2022-05-25
    Type: Article , NonPeerReviewed
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  • 3
    Publication Date: 2022-05-25
    Description: Microbial biomass, concentrations and enzymatic decomposition rates of proteins and carbohydrates were analysed during spring 1980 from a 10 m sediment station in the Kiel Bight. During March, a considerable increase in total organic matter, proteins and carbohydrates was observed, followed by a decrease during April. The accumulation of organic matter in the sediment could be traced back to the breakdown of the algal spring bloom. Activity rates of (α-amylase, ß-D-glucosidase and proteolytic enzymes paralleled the variation pattern in concentrations of carbohydrates and proteins. During the decomposition processes, a drastic shift in the protein to carbohydrate ratio was observed. The dominating part of the enzymes involved in the decomposition of organic material obviously arose from autolysis of the algae cells themselves. After an initial depression, microbial biomass increased, obviously due to the availability of suitable substrates for microbial growth following the decomposition processes.
    Type: Article , NonPeerReviewed
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  • 4
    Publication Date: 2022-05-17
    Description: This article describes and discusses a new method for the fluorescence microscopic examination of bacteria on Nuclepore membrane filters. The bacteria, which have been concentrated by filtration, are stained with a solution of acridine orange. Then the filters are destained with isopropyl alkohol and xylene. This method makes it possible to recognize small bacteria and to distinguish them from detritus. Dieser Beitrag beschreibt und diskutiert eine neue Methode zur fluoreszenzmikroskopischen Untersuchung von Bakterien auf Nuclepore-Membranfiltern. Die durch Filtration angereicherten Keime werden mit einer Akridinorange-Lösung gefärbt. AnschlieBend werden die Filter mit Isopropanol und Xylol entfärbt. Diese Methode ermöglicht es, auch kleine Bakterien zu erkennen und sie von Detritus zu unterscheiden.
    Type: Article , NonPeerReviewed
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  • 5
    Publication Date: 2022-05-23
    Type: Article , NonPeerReviewed
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