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  • MDPI Publishing  (9)
  • Massachusetts Institute of Technology and Woods Hole Oceanographic Institution  (2)
  • Blackwell Science Ltd  (1)
  • 1
    ISSN: 1471-4159
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Medizin
    Notizen: Abstract: A 20-kDa DNA-binding protein that binds the AT-rich sequences within the promoters of the brain-specific protein kinase C (PKC) γ and neurogranin/RC3 genes has been characterized as chromosomal nonhistone high-mobility-group protein (HMG)-I. This protein is a substrate of PKC α, β, γ, and δ but is poorly phosphorylated by PKC ε and ζ. Two major (Ser44 and Ser64) and four minor phosphorylation sites have been identified. The extents of phosphorylation of Ser44 and Ser64 were 1:1, whereas those of the four minor sites all together were 〈30% of the major one. These PKC phosphorylation sites are distinct from those phosphorylated by cdc2 kinase, which phosphorylates Thr53 and Thr78. Phosphorylation of HMG-I by PKC resulted in a reduction of DNA-binding affinity by 28-fold as compared with 12-fold caused by the phosphorylation with cdc2 kinase. HMG-I could be additively phosphorylated by cdc2 kinase and PKC, and the resulting doubly phosphorylated protein exhibited a 〉 100-fold reduction in binding affinity. The two cdc2 kinase phosphorylation sites of HMG-I are adjacent to the N terminus of two of the three predicted DNA-binding domains. In comparison, one of the major PKC phosphorylation sites, Ser64, is adjacent to the C terminus of the second DNA-binding domain, whereas Ser44 is located within the spanning region between the first and second DNA-binding domains. The current results suggest that phosphorylation of the mammalian HMG-I by PKC alone or in combination with cdc2 kinase provides an effective mechanism for the regulation of HMG-I function.
    Materialart: Digitale Medien
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 2
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    Unbekannt
    Massachusetts Institute of Technology and Woods Hole Oceanographic Institution
    Publikationsdatum: 2022-05-25
    Beschreibung: Submitted in partial fulfillment of the requirements for the degree of Master of Science at the Massachusetts Institute of Technology and the Woods Hole Oceanographic Institution September 1989
    Beschreibung: This thesis studies mixing and convection in a rectangular basin driven by a specified heat flux at the surface. A numerical model is constructed for this purpose. The main focus of the study is on the density and circulation structure resulting from the thermal forcing. In chapter two, a simple vertical one-dimensional model is developed to examine the mixing processes under a given surface heat flux. In order to simulate strong vertical mixing in the region where stratification is unstable, turbulent processes are modeled by a convective overturning parameterization of eddy viscosity and diffusivity. The results show that the density structure is strongly affected by the convective overturning adjustment as surface cooling prevails, and the resulting density field is nearly depth independent. In chapter three, a more complicated two-dimensional model is constructed to simulate mixing and circulation in a vertical rectangular basin with rigid boundaries. The aspect ratio of the basin ranges from 1 to 0.001 and Rayleigh number from 104 to 2 x 1012. It is found that the circulation pattern is dominated by these two important numbers. The roles of density overturning and density-momentum overturning mixing are further investigated. The results show that the convective overturning not only homogenizes the density field in the unstably stratified region but also contributes to increase the circulation. A crude scale analysis of the system shows that the characteristics of the density and momentum fields from the analysis agree well with the numerical results.
    Schlagwort(e): Ocean circulation
    Repository-Name: Woods Hole Open Access Server
    Materialart: Thesis
    Format: application/pdf
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 3
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    Unbekannt
    Massachusetts Institute of Technology and Woods Hole Oceanographic Institution
    Publikationsdatum: 2022-05-26
    Beschreibung: Submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy at the Massachusetts Institute of Technology and the Woods Hole Oceanographic Institution September 1992
    Beschreibung: This study concerns the barotropic interactions between a mesoscale eddy and a straight monotonic bottom topography. Through simple to relatively complicated modeling effort, some of the fundamental properties of the interaction are investigated. In chapter two, the fundamental aspects of the interaction are examined using a simple contour dynamics model. With the simplest model configuration of an ideal vortex and a step topography, the basic dynamical features of the observed oceanic eddy-topography interaction are qualitatively reproduced. The results consist of eddy-induced cross-topography exchange, formation of topographic eddies, eddy propagation and generation of topographic waves. In chapter three, a more complicated primitive equation model is used to investigate a mesoscale eddy interacting with an exponential continental shelf/slope topography on both f and β-planes. The f-plane model recasts the important features of chapter two. The roles of the eddy size and strength and the geometry of topography are studied. It is seen that the multiple anticyclonic eddy-slope interactions strongly affect the total cross-slope volume transport and the evolution of both the original anticyclone and the topographic eddy. Since a cyclone is trapped at the slope and eventually moves on to the slope, it is most effective in causing perturbation on the shelf and slope. The responses on the shelf and slope are mainly wavelike with dispersion relation obeying that of the free shelf-trapped wave modes. On the β-plane, the problem of an eddy colliding onto a continental shelf/slope from a distance with straight or oblique incident angles is investigated. It is found that the straight eddy incident is more effective in achieving large onslope eddy penetration distance than the oblique eddy incident. The formation of a dipole-like eddy pair consisting of the original anticyclone and the topographic cyclone acts to suppress the eddy decay due to long Rossby wave radiation. A weak along-slope current near the edge of the slope is found, which is part of a outer slope circulation cell originated from the Rossby wave wake trailing the propagating eddy. Model-observation comparisons in_chapter four show favorable qualitative agreement of the model results with some of the observed events in the eastern U.S. continental margins and in the Gulf of Mexico. The model results give dynamical interpretations to some observed features of the oceanic eddy-topography interactions and provide enlightening insight into the problem.
    Schlagwort(e): Eddies ; Ocean currents ; Fluid dynamics
    Repository-Name: Woods Hole Open Access Server
    Materialart: Thesis
    Format: application/pdf
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 4
    Publikationsdatum: 2018-05-15
    Beschreibung: Energies, Vol. 11, Pages 1242: Optimal Scheduling Strategies of Distributed Energy Storage Aggregator in Energy and Reserve Markets Considering Wind Power Uncertainties Energies doi: 10.3390/en11051242 Authors: Zengqiang Mi Yulong Jia Junjie Wang Xiaoming Zheng With continuous technological improvement and economic development of energy storage, distributed energy storage (DES) will be widely connected to the distribution network. If fragmented DES systems are aggregated to form a distributed energy storage aggregator (DESA), the DESA will have great potential to participate in the day-ahead energy and reserve market and the balancing market. The DESA could act as a mediator between the market and DES consumers, enabling beneficial coordination for DES owners and power systems. This paper presents a bilevel optimization model for DESAs in the energy and reserve market under wind power uncertainties. In the lower-level problem, generating companies, wind power plants (WPP), and DESAs are optimized for scheduling day-ahead (DA) energy and the reserve market. In the upper-level problem, operational strategies for DES systems and DESAs are designed to deal with wind power uncertainties in the balancing market. The DESA splits its resources between the energy and reserve markets so that it can reduce total power system consumption, and mutual profit for the system and end customers is achieved. This model is formulated as a mixed-integer linear programming (MILP) program, which can be solved with commercial software. The validity of the bilevel optimization model is verified by the eight-node test transmission system and IEEE-33 bus distribution system.
    Digitale ISSN: 1996-1073
    Thema: Energietechnik
    Publiziert von MDPI Publishing
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 5
    Publikationsdatum: 2018-03-09
    Beschreibung: IJGI, Vol. 7, Pages 85: Assessment of Multiple GNSS Real-Time SSR Products from Different Analysis Centers ISPRS International Journal of Geo-Information doi: 10.3390/ijgi7030085 Authors: Zhiyu Wang Zishen Li Liang Wang Xiaoming Wang Hong Yuan The real-time State Space Representation (SSR) product of the GNSS (Global Navigation Satellite System) orbit and clock is one of the most essential corrections for real-time precise point positioning (PPP). In this work, the performance of current SSR products from eight analysis centers were assessed by comparing it with the final product and the accuracy of real-time PPP. Numerical results showed that (1) the accuracies of the GPS SSR product were better than 8 cm for the satellite orbit and 0.3 ns for the satellite clock; (2) the accuracies of the GLONASS (GLObalnaya NAvigatsionnaya Sputnikovaya Sistema) SSR product were better than 10 cm for orbit RMS (Root Mean Square) and 0.6 ns for clock STD (Standard Deviation); and (3) the accuracies of the BDS (BeiDou Navigation Satellite System) and Galileo SSR products from CLK93 were about 14.54 and 4.42 cm for the orbit RMS and 0.32 and 0.18 ns for the clock STD, respectively. The simulated kinematic PPP results obtained using the SSR products from CLK93 and CLK51 performed better than those using other SSR products; and the accuracy of PPP based on all products was better than 6 and 10 cm in the horizontal and vertical directions, respectively. The real-time kinematic PPP experiment carried out in Beijing, Tianjin, and Shijiazhuang, China indicated that the SSR product CLK93 from Centre National d’Etudes Spatiales (CNES) had a better performance than CAS01. Moreover, the PPP with GPS + BDS dual systems had a higher accuracy than those with only a GPS single system.
    Digitale ISSN: 2220-9964
    Thema: Architektur, Bauingenieurwesen, Vermessung , Geologie und Paläontologie
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 6
    Publikationsdatum: 2018-02-16
    Beschreibung: Energies, Vol. 11, Pages 438: Power-Balancing Based Induction Machine Model for Power System Dynamic Analysis in Electromechanical Timescale Energies doi: 10.3390/en11020438 Authors: Ding Wang Xiaoming Yuan Meiqing Zhang Power balance, including active and reactive power, between the system supply and the demand from induction motor loads is a potentially necessary condition for system stable operation. Motion of system states depends on the balancing of active and reactive powers. Therefore, this paper proposes an induction machine model in electromechanical timescale from a power balancing viewpoint, in which the induction motor load is modeled as a voltage vector driven by power balancing between the system supply and the demand from induction motor load, so as to describe the dynamic characteristics of induction motor loads in a physical way for power system dynamic analysis. Then a voltage magnitude-phase dynamic analysis with the proposed induction machine model is constructed. Based on the voltage magnitude-phase dynamic analysis, the characteristics of grid-connected induction motor loads are explored, and the instability mechanisms of grid-connected induction motor loads induced by a large disturbance are discussed. It is shown that the dynamic behavior of grid-connected induction motor loads can be described as the dynamic process of the terminal voltage vector driven by coupled active and reactive power balancing in different timescales. In this way, the dynamic behavior of induction motor loads in terms of voltage magnitude-phase dynamics and its physical characteristics are clearly illustrated. Time-domain simulation results are presented to validate the above analyses.
    Digitale ISSN: 1996-1073
    Thema: Energietechnik
    Publiziert von MDPI Publishing
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 7
    Publikationsdatum: 2017-11-08
    Beschreibung: Water, Vol. 9, Pages 865: Cyanobacterial Nitrogen Fixation Influences the Nitrogen Removal Efficiency in a Constructed Wetland Water doi: 10.3390/w9110865 Authors: Xiaodong Zhang Xin Jia Liang Yan Jinzhi Wang Xiaoming Kang Lijuan Cui Nitrogen removal efficiency in constructed wetlands (CW) is influenced by multiple environmental factors. However, little is known about the role of cyanobacterial nitrogen fixation in affecting nitrogen removal efficiency. This study investigated how cyanobacterial nitrogen fixation affects the efficiency, at which a CW removes nitrogen from an associated artificial lake (AL) in Beijing. For this purpose, we measured cell densities of N-fixing and non-N-fixing cyanobacteria, the aquatic nitrogen fixation rate (RNfix), and the concentration of various nitrogen fractions over the growing season (April–November) of 2014 in both AL and CW. We found that the removal of particulate organic nitrogen (PON) contributed to >90% of the total nitrogen removal in the CW. The removal efficiency of PON was lower during August–October (55.45 ± 27.49%) than during April–July (68.86 ± 8.83%). Phytoplankton proliferation in summer, as one of the main sources of PON, may have exceeded the capacity of the CW and led to declines in PON removal efficiency. RNfix peaked in July–October (3–169 ng N·L−1·h−1) and was positively correlated with both PON concentration and the cell density of N-fixing Anabaena sp. over the growing season, suggesting that aquatic nitrogen fixation (primarily in the AL) may increase PON and thereby reduce the its removal efficiency in the CW.
    Digitale ISSN: 2073-4441
    Thema: Energietechnik
    Publiziert von MDPI Publishing
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 8
    Publikationsdatum: 2018-05-02
    Beschreibung: Sensors, Vol. 18, Pages 1385: Geometric Integration of Hybrid Correspondences for RGB-D Unidirectional Tracking Sensors doi: 10.3390/s18051385 Authors: Shengjun Tang Wu Chen Weixi Wang Xiaoming Li Walid Darwish Wenbin Li Zhengdong Huang Han Hu Renzhong Guo Traditionally, visual-based RGB-D SLAM systems only use correspondences with valid depth values for camera tracking, thus ignoring the regions without 3D information. Due to the strict limitation on measurement distance and view angle, such systems adopt only short-range constraints which may introduce larger drift errors during long-distance unidirectional tracking. In this paper, we propose a novel geometric integration method that makes use of both 2D and 3D correspondences for RGB-D tracking. Our method handles the problem by exploring visual features both when depth information is available and when it is unknown. The system comprises two parts: coarse pose tracking with 3D correspondences, and geometric integration with hybrid correspondences. First, the coarse pose tracking generates the initial camera pose using 3D correspondences with frame-by-frame registration. The initial camera poses are then used as inputs for the geometric integration model, along with 3D correspondences, 2D-3D correspondences and 2D correspondences identified from frame pairs. The initial 3D location of the correspondence is determined in two ways, from depth image and by using the initial poses to triangulate. The model improves the camera poses and decreases drift error during long-distance RGB-D tracking iteratively. Experiments were conducted using data sequences collected by commercial Structure Sensors. The results verify that the geometric integration of hybrid correspondences effectively decreases the drift error and improves mapping accuracy. Furthermore, the model enables a comparative and synergistic use of datasets, including both 2D and 3D features.
    Digitale ISSN: 1424-8220
    Thema: Chemie und Pharmazie , Elektrotechnik, Elektronik, Nachrichtentechnik
    Publiziert von MDPI Publishing
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 9
    Publikationsdatum: 2016-12-31
    Beschreibung: Enterovirus 71 (EV71) usually causes hand-foot-and-mouth disease (HFMD) with severe clinical symptoms and even deaths in China. There is no efficient antiviral drug to protect against severe EV71-associated HFMD, making the development of EV71 vaccines therefore a priority. However, the potential target subject population(s) to be immunized with EV71 vaccine are not well understood. In this study, we characterized the epidemiology regarding EV71-associated HFMD on the basis of provincial-level surveillance. We extracted data on EV71-associated HFMD from the National Notifiable Disease Reporting System in Zhejiang Province, China between 1 January 2009 and 31 December 2013 (n = 7650). The higher incidence rate of EV71 cases occurred in those children aged 12–23 months, with boys being predominant. Interestingly, different peaks activities of EV71 infection was observed in different calendar year, with one peak in 2009 and 2013 and two peaks in 2010–2012. However, EV71 infection seemed to predominately occur in warm season and a distinguished cyclic peak that seemed to be of about 12 months. Children aged 12–23 months are thus identified as an important target population for public health intervention, for example, it is recommended that these key subjects immunized with EV71 vaccine. In addition, an enhanced surveillance system for EV71-associated with HFMD needs to focus on generic and phylogenetic analysis.
    Print ISSN: 1661-7827
    Digitale ISSN: 1660-4601
    Thema: Energietechnik , Medizin
    Publiziert von MDPI Publishing
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 10
    Publikationsdatum: 2017-01-22
    Beschreibung: To meet the great needs for MFL (magnetic flux leakage) inspection of drill pipes at wellheads, a lift-off-tolerant MFL testing method is proposed and investigated in this paper. Firstly, a Helmholtz coil magnetization method and the whole MFL testing scheme are proposed. Then, based on the magnetic field focusing effect of ferrite cores, a lift-off-tolerant MFL sensor is developed and tested. It shows high sensitivity at a lift-off distance of 5.0 mm. Further, the follow-up high repeatability MFL probing system is designed and manufactured, which was embedded with the developed sensors. It can track the swing movement of drill pipes and allow the pipe ends to pass smoothly. Finally, the developed system is employed in a drilling field for drill pipe inspection. Test results show that the proposed method can fulfill the requirements for drill pipe inspection at wellheads, which is of great importance in drill pipe safety.
    Digitale ISSN: 1424-8220
    Thema: Chemie und Pharmazie , Elektrotechnik, Elektronik, Nachrichtentechnik
    Publiziert von MDPI Publishing
    Standort Signatur Einschränkungen Verfügbarkeit
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