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  • 1970-1974  (5)
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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 23 (1970), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Metabolites of tryptophan were investigated using 2 systems: a bacterial (Peastem homogenates containing the epiphytic bacteria) and a plant system (pea stem sections under sterile conditions).The plant system produces: indolepyruvic acid (IPyA), indoleacetaldehyde (IAAld) indoleacetic acid (IAA), indoleethanol (tryptophol, IAAol), indolecarboxylie acid (ICA), indolecarboxaldehyde (ICAld). Bacteria produce additionally: indoleactic acid (ILA), tryptamine (TNH2) and the unknown Xb and Yb, but IAAld was not detected.A nonacidic inhibitor extract from pea stems decreases the gain of IAA, IPyA, ILA, Yb. It increases the gain of IAAld, IAAol, TNH2, Xb, and (only in the bacterial system) ICA and ICAld. Three sites of inhibitor action are suggested, namely the steps Try → IPyA, TNH2→ IAAld, IAAld → IAA.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 23 (1970), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The auxin content (extractable and ‘diffusible’ auxin) of non-sterile corn plants is much more increased by a tryptophan application than the auxin content of sterile plants. This effect is independent of the mode of tryptophan application (spray or supply with the transpiration stream). The epiphytic bacteria settling the shoot surface are responsible for this effect, since in special experiments the rhizosphere was separated from the tryptophan treatment. Sterilized plants which were artificially reinfected with epiphytic IAA-producing bacteria strains behave like non-sterile plants. Non-sterile plants which were superinfected with these bacteria strains have a still higher capacity to convert tryptophan to auxin.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 23 (1970), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: More “diffusible” auxin is received from nonsterile than from sterile corn coleoptile tips. An artificial reinfection of sterile coleoptiles with epiphytic, IAA-producing bacteria strains does, a superinfection of nonsterile coleoptiles does not increase the auxin amount. The difference between sterile and nonsterile tips persists if diffusion from the coleoptile surface is excluded by covering the surface with a paraffin layer. The greater the distance from the apex, the higher becomes the superiority of nonsterile tips. An artificial bacterial contamination of the contact face between tip and receiver agar block, or addition of glucose and tryptophan to the agar block, do not influence the received auxin amount. Consequently the additional, bacteria-produced auxin delivered by the nonsterile tip is not produced at the cut surface or in the agar but is present in the tissues of the coleoptile tip.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 23 (1970), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Metabolites of indoleacetaldehyde (IAAld), indoleethnol (tryptophol, IAAoI), tryptamine (TNH2) and indoleacetamide (IAAm) were investigated using 2 systems; a baeterial (Pea stem homogenates containing the epiphytic bacteria) and a plant system (pea stem sections under sterile conditions). In both systems, indoleacetic acid (IAA), IAAol and indolecarboxylic acid (ICA) are produced from IAAld, and IAA and ICA are produced from IAAol (production of IAAld and ICAld not investigated). In the plant system TNH2 is converted to IAAld,IAA, IAAol and little ICA and ICAld. In the bacterial system TNH2 is converted to IAA, IAAol, the unknown substance Xb, and many ICA and ICAld. Xb is produced from IAAm, too. A nonacidie inhibitor extract from pea stems reduces the IAA gain from IAAld, IAAol and TNH2, and the IAAol and IAAld gain from TNH2; in the bacterial system it increases the IAAol gain from IAAld and the Xb gain from TNH2 and IAAm. Metabolic routes connecting Try, TNH2 and IAA are constructed for the plant tissue and for the epiphytic bacteria, respectively, including 4 sites of inhibitor action.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 23 (1970), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Epiphytic, IAA-producing bacteria strains were fed with 14C-tryptophan (Try). 14C-Try absorption and, after transfer to a Try-free medium, 14C-IAA output were stated. Using 4 different methods, the 14C-Try containing bacteria were applied to the tips of sterile corn coleoptiles and the ‘diffusible’ auxin collected at the coleoptile bases by means of agar blocks. 14C-IAA was detected in the agar blocks. Sterile coleoptiles the tips of which were wupplied with 14C-Try also deliver some 14C-IAA at their bases, but much less than both sterile coleoptiles supplied with 14C-Try-containing bacteria and nonsterile supplied with 14C-Try.
    Type of Medium: Electronic Resource
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