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  • 1995-1999  (3)
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  • 1
    Electronic Resource
    Electronic Resource
    Detection of p34cdc2- and cyclin B-like proteins in Dunaliella tertiolecta (Chlorophyceae) (1996)
    Springer
    Marine biology 125 (1996), S. 603-610 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract p34cdc2 and cyclin B are two key proteins in the eukaryotic cell cycle control machinery. They thus could be important cell cycle markers for studies of environmental effects on cell cycle progression and on growth rate of marine phytoplankton. From July 1993 to March 1995, we used commercially available antibodies to examine the presence of their homologs in a marine phytoplankton species, Dunaliella tertiolecta Butcher. A p34cdc2-like protein was detected on the Western blots, with an apparent molecular mass as expected (34 kDa). Anti-cyclin B detected a protein of 63 kDa, a size similar to that of cyclin B in other organisms. The two proteins decreased from the exponential to the stationary growth phase. As determined on the Western blots, their abundance only changed slightly during the cell cycle, being slightly more abundant prior to cell division. Immunofluorescence performed for a partially synchronized culture showed that the fraction of the cell population that was positively stained by anti-p34cdc2 was highest at the time when the culture was mainly in the late G1 or early S phase, and in the late G2 or early M phase, respectively. The fraction was low when the culture was mainly in the S phase. Although further characterization is required to verify their identities, these two growth phase-related proteins appear to be p34cdc2 and cyclin B homologs, which may be useful in studying the cell cycle and growth rates of phytoplankton.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00353271
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Rubisco of Dunaliella tertiolecta is redistributed between the pyrenoid and the stroma as a light /shade response (1997)
    Springer
    Marine biology 127 (1997), S. 521-529 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  We used an immunofluorescence technique to investigate the effects of varying light regimes on ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) in individual cells of the chlorophyte Dunaliella tertiolecta Butcher. These studies were carried out between September 1995 and February 1996. The population was heterogeneous with respect to Rubisco localization in the pyrenoid, i.e. in some cells Rubisco staining was highly concentrated in the pyrenoid while in others it was evenly distributed throughout the chloroplast stroma. When light intensity was varied sevenfold, the fraction of the cell population that displayed distinct Rubisco staining in the pyrenoid (PR-index) was correlated with light intensity, although the average Rubisco abundance per cell or per total cellular protein appeared fairly constant. In darkness, or when treated with 3-(3,4-dichlorophenyl)-1,1-dimethyl urea (DCMU), the PR-index decreased markedly during the first 4 and 3 h, respectively, and then remained at low levels, while the cell division cycle progression remained unaffected. We conclude that D. tertiolecta probably possesses an adaptive mechanism, i.e. the redistribution of Rubisco between the pyrenoid (probably the active site of Rubisco activation and CO2 fixation) and the stroma (probably a reservoir of deactivated Rubisco that is readily available for transport to the pyrenoid and activation), to respond to variations in irradiance or photosynthetic inhibition. Our results also suggest that this mechanism is insensitive to slight variations in growth irradiance and to seasonal changes in photoperiod and temperature.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002270050041
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    No UVB effect? (1995)
    [s.l.] : Nature Publishing Group
    Nature 374 (1995), S. 601-601 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] SIR - McMinn et al.1 have examined the relative abundances of diatom taxa in three sediment cores, each taken from a different fjord in the Vestfold Hills region of eastern Antarctica. Over a 20-year period they noted little change in species composition based on relative abundances, and they ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/374601a0
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    Paper (German National Licenses)
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