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  • 1
    Publication Date: 2023-02-12
    Keywords: Calculated after Luo et al. (2012); Comment; CTD/Rosette; CTD-RO; Date/Time of event; DEPTH, water; Diazotrophs, total biomass as carbon; East China Sea; Event label; Latitude of event; Light microscope; Longitude of event; MAREDAT_Diazotrophs_Collection; OR-I/414_1; OR-I/414_2; OR-I/448; OR-II/034; OR-II/111_1; OR-II/111_2; OR-II/149_1; OR-II/149_2; Trichodesmium, biomass as carbon; Trichodesmium, carbon per trichome; Trichodesmium abundance, total
    Type: Dataset
    Format: text/tab-separated-values, 425 data points
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  • 2
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Industrial and engineering chemistry 21 (1982), S. 101-106 
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Marine biology 125 (1996), S. 603-610 
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract p34cdc2 and cyclin B are two key proteins in the eukaryotic cell cycle control machinery. They thus could be important cell cycle markers for studies of environmental effects on cell cycle progression and on growth rate of marine phytoplankton. From July 1993 to March 1995, we used commercially available antibodies to examine the presence of their homologs in a marine phytoplankton species, Dunaliella tertiolecta Butcher. A p34cdc2-like protein was detected on the Western blots, with an apparent molecular mass as expected (34 kDa). Anti-cyclin B detected a protein of 63 kDa, a size similar to that of cyclin B in other organisms. The two proteins decreased from the exponential to the stationary growth phase. As determined on the Western blots, their abundance only changed slightly during the cell cycle, being slightly more abundant prior to cell division. Immunofluorescence performed for a partially synchronized culture showed that the fraction of the cell population that was positively stained by anti-p34cdc2 was highest at the time when the culture was mainly in the late G1 or early S phase, and in the late G2 or early M phase, respectively. The fraction was low when the culture was mainly in the S phase. Although further characterization is required to verify their identities, these two growth phase-related proteins appear to be p34cdc2 and cyclin B homologs, which may be useful in studying the cell cycle and growth rates of phytoplankton.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Pediatric cardiology 13 (1992), S. 52-55 
    ISSN: 1432-1971
    Keywords: Cardiac tumor ; Hemangioendothelioma ; Cardiac arrhythmia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A 4-month-old infant with cardiac hemangioendothelioma presented with thrombocytopenia, and pericardial effusion, as well as signs and symptoms of heart failure. This is the first reported case of infantile cardiac hemangioma successfully treated with steroids.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1436-2236
    Keywords: Key words: algae, cell cycle, diatoms, DNA polymerase alpha, PCR, phytoplankton.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract: To investigate the potential of DNA polymerase α as a marker for DNA replication in phytoplankton, two gene fragments that showed a high degree of similarity with eukaryotic DNA polymerase α were cloned from two strains of a diatom, Skeletonema costatum (Greville) Cleve. The gene fragments amplified with the polymerase chain reaction were 397 and 396 bp in length, respectively. The deduced amino acid sequences showed 44% to 61% similarity to the corresponding regions of DNA polymerase α sequences of eukaryotic organisms ranging from yeast to humans. The similarity was especially high in three evolutionarily conserved regions within the amplified fragments. Further, hybridization patterns from Southern blotting confirmed that the amplified fragments were an integral part on the genome of S. costatum. In batch cultures abundant messenger of DNA polymerase α appeared in the late exponential phase and the early stationary phase. This pattern suggests that DNA polymerase α expression is associated with actively dividing cells.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1573-6903
    Keywords: L-α-aminoadipate ; kynurenate ; kynurenine ; aminotransferase ; astrocyte ; neurodegeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Intracerebral administration of L-α-aminoadipic acid (L-AAA) at 500 mg/kg body weight to rats caused a complex behavioral change with sporadic wet-dog shakes. Animals developed severe limbic seizures between 1 and 6 h after L-AAA injection, characterized by generalized convulsions. Twenty days after L-AAA injection kynurenine aminotransferase (KAT) activity measured in hippocampal brain tissue slices prepared with a McIlwain chopper at 30 μm showed a significant 43% decrease. Subcutaneous injection of kynurenine at 500 mg/kg showed a 63% increase in KAT activity twenty days later. This increase was offset by a concomitant administration of 500 mg/kg L-AAA stereotaxically on day one. In astrocyte culture kynurenic acid synthesis is inhibited by L-AAA and L-pipecolic acid. The possible involvement of kynurenic acid in the modulation of neuronal degeneration is discussed.
    Type of Medium: Electronic Resource
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  • 7
    Publication Date: 2018-08-15
    Description: Observations of the dinoflagellate Dinophysis norvegica in the Baltic Sea during the summers of 1991–1993 indicate that maximal abundances (c 40–150 × 103 cells l-1) were found at the thermocline, typically at 12°C. Maximum densities were usually between 12 and 15 m where 2·9% and 1·5% of surface photon irradiances, respectively, were measured. No diel vertical migration was observed, and cell densities in the mixed layer were always low. Photosynthesis versus irradiance measurements with an oxygen electrode indicated that these populations had a P max of 2·47 [coefficient of variation (CV) 7·3%] and 3·4 (CV 4·7%) mg O2 mg Chl a -1 h-1, and compensation values of photon irradiance were 16·5 and 83 μmol m-2 s-1 in 1992 and 1993, respectively. Both oxygen electrode and 14C light/dark bottle measurements indicated that D. norvegica had very little net photosynthesis at the depths where it was most abundant; it would have had about 2·5-fold greater capacity at photon irradiances present closer to the surface. Calculated carbon doubling times via photosynthesis averaged 4–11 months. There was no observable diel rhythym of DNA synthesis, suggesting that either D. norvegica was not dividing synchronously (asynchronous division is common in heterotrophs) or not dividing at all. Electron microscopy did not reveal the presence of food vacuoles, but feeding and digestion could have been extracellular. The data suggest that this species is a mixotroph which received its primary nutrition via heterotrophic means during our observation periods in the summers of 1991–1993.
    Type: Article , PeerReviewed
    Format: text
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  • 8
    Publication Date: 2018-05-07
    Description: The accuracy of species-specific phytoplankton growth rates estimated by cell cycle analysis was tested with the dinoflagellate Prorocentrum minimum (Pav.) Sch. under conditions of altered nitrogen and phosphorus availability. Reduced nutrient availability caused major changes in the duration of cell cycle phases. At the nutrient level of complete f/2 media, the length of the combination of S, G2, and M phases was about 8 h at growth rates of 0.53 to 0.56 d-' A decrease in ~ 0 ,o~r N-O3 concentration extended the S+G2+M phase to about 15.5 to 17.7 h at growth rates ranging from 0.41 to 0.30 d-' Changes in phase durations dld not significantly affect growth rate estimates. In addition, a minimum growth rate, calculated from the maximum values on phase fraction curves, was shown to be usable as an error detector in some cases. Results support the validity of cell cycle analysis to measure in situ growth rates.
    Type: Article , PeerReviewed
    Format: text
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