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  • 1
    Online Resource
    Online Resource
    American Society for Microbiology ; 2004
    In:  Applied and Environmental Microbiology Vol. 70, No. 7 ( 2004-07), p. 4144-4150
    In: Applied and Environmental Microbiology, American Society for Microbiology, Vol. 70, No. 7 ( 2004-07), p. 4144-4150
    Abstract: The large tropical lucinid clam Codakia orbicularis has a symbiotic relationship with intracellular, sulfide-oxidizing chemoautotrophic bacteria. The respiration strategies utilized by the symbiont were explored using integrative techniques on mechanically purified symbionts and intact clam-symbiont associations along with habitat analysis. Previous work on a related symbiont species found in the host lucinid Lucinoma aequizonata showed that the symbionts obligately used nitrate as an electron acceptor, even under oxygenated conditions. In contrast, the symbionts of C. orbicularis use oxygen as the primary electron acceptor while evidence for nitrate respiration was lacking. Direct measurements obtained by using microelectrodes in purified symbiont suspensions showed that the symbionts consumed oxygen; this intracellular respiration was confirmed by using the redox dye CTC (5-cyano-2,3-ditolyl tetrazolium chloride). In the few intact chemosymbioses tested in previous studies, hydrogen sulfide production was shown to occur when the animal-symbiont association was exposed to anoxia and elemental sulfur stored in the thioautotrophic symbionts was proposed to serve as an electron sink in the absence of oxygen and nitrate. However, this is the first study to show by direct measurements using sulfide microelectrodes in enriched symbiont suspensions that the symbionts are the actual source of sulfide under anoxic conditions.
    Type of Medium: Online Resource
    ISSN: 0099-2240 , 1098-5336
    RVK:
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 2004
    detail.hit.zdb_id: 223011-2
    detail.hit.zdb_id: 1478346-0
    SSG: 12
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  • 2
    In: Microbiology, Microbiology Society, Vol. 146, No. 6 ( 2000-06-01), p. 1287-1293
    Type of Medium: Online Resource
    ISSN: 1350-0872 , 1465-2080
    Language: English
    Publisher: Microbiology Society
    Publication Date: 2000
    detail.hit.zdb_id: 2008736-6
    SSG: 12
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  • 3
    In: Geochemistry, Geophysics, Geosystems, American Geophysical Union (AGU), Vol. 4, No. 12 ( 2003-12)
    Abstract: As part of an ongoing effort to explore the use of foraminifera as a means to assess modern and ancient methane release, we compared ambient pore water chemistry with the distribution and stable isotopic composition of living (rose Bengal stained) foraminifera in MBARI ROV Ventana tube cores taken from modern seepage areas (about 1000 m water depth) in Monterey Bay, California. Benthic foraminiferal isotopic differences between sites clearly indicate that methane‐influenced pore waters affect foraminiferal distributions and carbonate isotope geochemistry. Carbon isotope signatures of living benthic foraminifera did not conform to the very negative (−30 to −48‰), methane‐influenced carbon isotope values of the pore waters they live in. Instead, the influence of methane seep pore waters was reflected in the greater range and carbon isotopic variability of living seep foraminifera compared with published δ 13 C values of foraminifera living in nonseep habitats. It is not clear what relative influences biological, ecological, and physical factors have on the carbon isotopic signatures observed in seep foraminifera. Substantial carbon isotope differences can exist between individuals of the same seep species. For instance, δ 13 C values of living Globobulimina pacifica varied by as much as 2.9‰ between seeps within 8 km of each other, whereas δ 13 C values of living Uvigerina peregrina varied by as much as 1.95‰ within the same seep. Provided there is no diagenetic alteration of the test carbonate, isotopic results of individual seep foraminifera support the hypothesis that foraminifera can be used to assess past and present methane seepage.
    Type of Medium: Online Resource
    ISSN: 1525-2027 , 1525-2027
    Language: English
    Publisher: American Geophysical Union (AGU)
    Publication Date: 2003
    detail.hit.zdb_id: 2027201-7
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