In:
Chemical Biology & Drug Design, Wiley, Vol. 91, No. 1 ( 2018-01), p. 17-28
Abstract:
Water‐soluble arginyl–diosgenin (Arg‐ DG ) conjugate was designed, synthesized, and evaluated for a biological activity. The Arg‐ DG conjugate was characterized using FT ‐ IR , 1 H NMR , 13 C NMR , and HPLC ‐ MS analyses, followed by a biological activity evaluation. Compared with DG , the Arg‐ DG conjugate showed a decreased cytotoxicity against L929 cells and an increased antiproliferative activity against hepatocellular cells. The safety of the Arg‐ DG conjugate was confirmed using the highly sensitive Alamar Blue assay, which indicated that it increased the cellular metabolic activity at suitable concentrations. The Arg‐ DG conjugate promoted an endothelial tube formation as well. Furthermore, the Arg‐ DG conjugate improved the bone morphogenetic protein 2 ( BMP 2)‐induced osteoblastic differentiation with synergistic effects on alkaline phosphatase ( ALP ) activity and mineralization. These results suggest that the Arg‐ DG conjugate developed in this study has great potentials for biomedical applications such as bone tissue engineering.
Type of Medium:
Online Resource
ISSN:
1747-0277
,
1747-0285
DOI:
10.1111/cbdd.2018.91.issue-1
Language:
English
Publisher:
Wiley
Publication Date:
2018
detail.hit.zdb_id:
2216600-2
SSG:
12
Permalink