In:
MicroRNA, Bentham Science Publishers Ltd., Vol. 11, No. 3 ( 2022-11), p. 216-226
Abstract:
The fetus grows in a sterile womb environment. After birth, the newborn
immune system has two immediate hurdles to clear. First immediate suppression of the womb compatible immune system and turn on the immune system of the newborn that can counter the antigenic
world. The underlying mechanism of immune fluctuation by milk microRNAs (miRNAs) can be crucial for the treatment of critical or premature newborn. Methods: We collected fourteen samples of each colostrum and mature milk from lactating mothers,
four samples of each were used for microarray analysis, and the other ten were used for miRNA expression profiling by real-time PCR. Results: From the microarray, 154 differentially expressed miRNAs were identified, whereas 49
miRNAs were revealed as immune-related miRNAs based on a literature study. Among the 49 miRNAs, 33 were already shown as strongly validated immune-related miRNAs (validated by
qPCR, Western Blot, and Luciferase assay) and were considered for further analysis. Twenty-two miRNA expressions were analysed by real-time PCR as their Ct values were within considerable
limits. Twelve numbers of miRNAs were significantly downregulated in mature milk compared to colostrum, which were again subjected to bioinformatics analysis to predict the biological mechanisms
behind the differentially expressed miRNAs. Conclusion: This study shed light on the human milk exosome miRNA expression dynamics during
lactation and their possible role in the gradual skewing of the newborns' immune system. The information is crucial for the development and onset of sepsis in premature newborns in the NICU.
Type of Medium:
Online Resource
ISSN:
2211-5366
DOI:
10.2174/2211536611666220630102316
Language:
English
Publisher:
Bentham Science Publishers Ltd.
Publication Date:
2022
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