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  • 1
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    Ocorrência de paratuberculose em búfalos (Bubalus bubalis) em Pernambuco
    FapUNIFESP (SciELO) ; 2010
    In:  Pesquisa Veterinária Brasileira Vol. 30, No. 3 ( 2010-03), p. 237-242
    Details
    In: Pesquisa Veterinária Brasileira, FapUNIFESP (SciELO), Vol. 30, No. 3 ( 2010-03), p. 237-242
    Abstract: Paratuberculosis (PTB) is a disease of great economical importance for ruminant in several countries and represents a threat to the development of Brazilian livestock. The contagious disease caused by chronic PTB leads to incurable granulomatous enterocolitis of difficult control. PTB is caused by the Mycobacterium avium subsp. paratuberculosis (MAP). No record on the occurrence of paratuberculosis in buffaloes in Brazil could be found. Five of 100 buffaloes in a herd in Pernambuco-Brazil showed clinical signs characteristic of PTB. At necropsy, of two animals the lesions were restricted to the small intestine with thickening and corrugation of the mucosa, increase of mesenteric lymph nodes and prominent lymph vessels. Histopathology revealed granulomatous inflammation infiltrated with numerous epithelioid macrophages, Langhans type giant cells, and clusters of Ziehl-Neelsen (ZN) positive organisms within the intestinal mucosa. In the mesenteric lymph nodes there was thickening of the capsule and marked granulomatous inflammation. Smears of feces and scrapping smears were prepared from intestinal mucosa and cut surface of mesenteric lymph nodes and, stained by the Ziehl-Neelsen method for research of acid fast bacilli, with positive results. Lymph nodes and intestinal mucosa revealed at IS900 specific polymerase chain reaction amplification of a fragment of about 110pb, confirmed by the comparison with other sequences of M. avium subsp. paratuberculosis available in GenBank.
    Type of Medium: Online Resource
    ISSN: 0100-736X
    URL: Article
    DOI: 10.1590/S0100-736X2010000300008
    Language: Unknown
    Publisher: FapUNIFESP (SciELO)
    Publication Date: 2010
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  • 2
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    Paratuberculose em ruminantes no Brasil
    FapUNIFESP (SciELO) ; 2013
    In:  Pesquisa Veterinária Brasileira Vol. 33, No. 2 ( 2013-02), p. 127-140
    Details
    In: Pesquisa Veterinária Brasileira, FapUNIFESP (SciELO), Vol. 33, No. 2 ( 2013-02), p. 127-140
    Abstract: Paratuberculosis also known as Johne's disease, is a granulomatous enteritis caused by Mycobacterium avium subsp. paratuberculosis (MAP), an acid-fast bacillus that preferentially resides within host intestinal macrophages. The condition is most commonly seen in domestic ruminants, however MAP can also infect other mammalian species. Paratuberculosis shows a global distribution and is considered endemic in some regions. The World Organization for Animal Health (OIE, Office International des Epizooties), have classified paratuberculosis as a notificable disease; considered to be of socio-economic and/or public-health importance, the control of which is necessary for the international trade of animal and animal products. The importance of paratuberculosis is related primarily to economic losses in the animal industry and also because of a potential role for this bacterium in the pathogenesis of Crohn´s disease, a debilitating condition affecting the digestive tract of humans. In Brazil, paratuberculosis has been reported in a variety of ruminant species and shows a broad geographic distribution. The reported incidence of natural cases in Brazil has been limited, but it is believed that interespecific transmission of MAP and dissemination of the agent is driven by the commercialization of infected animals. The main objective of this paper was to collate the published epidemiological, clinic-pathological and diagnostic information in relation to paratuberculosis in cattle, buffaloes, goats and sheep in Brazil. Moreover, it served as a platform to emphasize the requirement to implement sanitary policies for control of MAP in the county, which may serve to improve the quality and value of animal products on international markets.
    Type of Medium: Online Resource
    ISSN: 0100-736X
    URL: Article
    DOI: 10.1590/S0100-736X2013000200001
    Language: Unknown
    Publisher: FapUNIFESP (SciELO)
    Publication Date: 2013
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  • 3
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    Lesões traumáticas de pele causadas pelos espinhos de Mimosa pudica e Mimosa debilis em equídeos
    FapUNIFESP (SciELO) ; 2011
    In:  Pesquisa Veterinária Brasileira Vol. 31, No. 9 ( 2011-09), p. 768-772
    Details
    In: Pesquisa Veterinária Brasileira, FapUNIFESP (SciELO), Vol. 31, No. 9 ( 2011-09), p. 768-772
    Abstract: Studies on the nature and cause of skin lesions in horses on a farm in the county of Castanhal, northeastern Para, Brazil were conducted. These were visits to the farm, epidemiological studies, blood sample collections, skin biopsies, and inspection of the pasture. The study included 25 Equidae, 14 males and 11 females, six months to eight years old. The animals showed ulcerative lesions of irregular borders on the head (nose, muzzle, upper and lower lips), in the oral cavity (buccal vestibule and gum) and on the limbs (billets, metacarpals and metatarsals and scapular-humeral joint). The histopathological examination revealed foci of cutaneous erosions with epidermal necrosis, spongiosis and vesicular degeneration of the remaining epidermis, and mild inflammatory infiltrate in the underlying dermis, consisting predominantly of macrophages and, to a lesser degree, eosinophils. The inspection of the pasture, which consisted of Brachiaria humidicola, revealed a heavy invasion by two plants provided with spines, Mimosa pudica and Mimosa debilis, of the Leguminosae Mimosoideae family. It was concluded that the skin lesions were caused by traumatic action of the spines of Mimosa pudica and Mimosa debilis.
    Type of Medium: Online Resource
    ISSN: 0100-736X
    URL: Article
    DOI: 10.1590/S0100-736X2011000900008
    Language: Unknown
    Publisher: FapUNIFESP (SciELO)
    Publication Date: 2011
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  • 4
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    Surto de encefalomielite equina Leste na Ilha de Marajó, Pará
    FapUNIFESP (SciELO) ; 2013
    In:  Pesquisa Veterinária Brasileira Vol. 33, No. 4 ( 2013-04), p. 443-448
    Details
    In: Pesquisa Veterinária Brasileira, FapUNIFESP (SciELO), Vol. 33, No. 4 ( 2013-04), p. 443-448
    Abstract: Nine cases of equine encephalomyelitis on Marajó Island, state of Pará, Brazil, were studied. The affected horses had difficulty to stand, walked in circles, with marked depression, closed eyelids, tongue paralysis, muscle tremors, bruxism, anorexia and dehydration. Some had their ear and eyelid reflexes diminished, decreased tongue tone and tachycardia; laid down frequently they kept their head on the chest. Often they were seen resting their head on tree trunks or fences. At necropsy, hemorrhages of the meninges and spinal cord, and in some animals also adhesion of the meninges were found. Histologically there was diffuse encephalitis affecting mainly the gray matter, with meningitis and choroiditis. Presence of perivascular cuffs consisting of mononuclear inflammatory cells was observed. From two horses the Eastern equine encephalitis virus was identified by semi-nested reverse transcription polymerase chain reaction (semi-nested RT-PCR).
    Type of Medium: Online Resource
    ISSN: 0100-736X
    URL: Article
    DOI: 10.1590/S0100-736X2013000400005
    Language: Unknown
    Publisher: FapUNIFESP (SciELO)
    Publication Date: 2013
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  • 5
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    Aspectos clinicopatológicos e laboratoriais do envenenamento experimental por Bothrops moojeni e Bothropoides neuwiedi em ovinos
    FapUNIFESP (SciELO) ; 2012
    In:  Pesquisa Veterinária Brasileira Vol. 32, No. 1 ( 2012-01), p. 49-60
    Details
    In: Pesquisa Veterinária Brasileira, FapUNIFESP (SciELO), Vol. 32, No. 1 ( 2012-01), p. 49-60
    Abstract: Spontaneous envenoming by snake bite is described as a cause of death in domestic animals. However, there are just few information about the species of snake involved, course, and clinicopathological and laboratory findings. Thus, this research aimed to determine the clinicopathological and laboratory changes induced by Bothrops moojeni and Bothropoides neuwiedi snake venoms in sheep, in order to provide additional information regarding snakebites in farm animals and to help establish the diagnosis of this condition. The lyophilized snake venoms were dissolved in 1mL saline solution and administered subcutaneously into the right face of four sheep, at doses of 0.41mg/kg and 0.82mg/kg of B. moojeni venom for two sheep, and 1.0mg/kg of B. neuwiedi venom for two other sheep. Only the sheep which had received the lowest dose (0.41mg/kg) survived, but developed severe clinical signs, similar to the others. First clinical signs were observed about 10 minutes after inoculation in all sheep. The course varied from 2 to 4 days. The clinical findings in all sheep were characterized by apathy, marked swelling of the face, the ventral neck and esternal region, and mild swelling of the proximal portion of the forelimbs, as well as increased bleeding time, tachycardia, pale mucous membranes, and large quantity of undigested blood in the intestinal lumen. Laboratory exams showed mainly a reduction in serum protein and increased creatine kinase in all sheep. At necropsy, extensive hematomas were observed in the subcutaneous tissue of the swollen areas. Also petechiae, bruises and mild to moderate hemorrhagic suffusions on the serosa of various organs, and blood within the intestinal contents of the distal rectum were observed. In addition to hemorrhages, the main histopathological changes were necrosis of skeletal muscle fibers and blood vessel walls next to the inoculation site. The swollen areas on face, neck, sternum and limbs of the sheep were due the hematomas.
    Type of Medium: Online Resource
    ISSN: 0100-736X
    URL: Article
    DOI: 10.1590/S0100-736X2012000100009
    Language: Unknown
    Publisher: FapUNIFESP (SciELO)
    Publication Date: 2012
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  • 6
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    Diagnóstico imuno-histopatológico da paratuberculose subclínica em bovinos no estado do Rio de Janeiro
    FapUNIFESP (SciELO) ; 2013
    In:  Pesquisa Veterinária Brasileira Vol. 33, No. 12 ( 2013-12), p. 1427-1432
    Details
    In: Pesquisa Veterinária Brasileira, FapUNIFESP (SciELO), Vol. 33, No. 12 ( 2013-12), p. 1427-1432
    Abstract: The early and specific diagnosis of paratuberculosis remains a challenge due to the low sensitivity of the currently available laboratory tests and also because of variations in the immune response towards infection with Mycobacterium avium subsp. paratuberculosis. Globally this disease causes significant economic losses, primarily in dairy cattle, owing to the chronic nature of the infection. Paratuberculosis has been described in a number of Brazilian states and from a diversity of domestic ruminant species clearly demonstrating that the disease is present in the country and highlighting the requirement for the development of diagnostic techniques for confirmation of infection and for epidemiological analyses. The aim of this study was to characterize the anatomo-histopathological and immunohistochemical findings in the bowel and mesenteric lymph nodes of assymptomatic cattle, derived from paratuberculosis positive herds located in state of Rio de Janeiro, Brazil. Macroscopic examination during necropsy revealed nonspecific changes including reddening of the gut mucosa, increased volumes for the Peyer's patches and mesenteric lymph nodes and in some case dilation and whitening of the mesenteric lymphatic vessel. Histopathology revealed granulomatous infiltration, occasionally with the formation of giant cells in the jejunal and ileal mucosa or sub-mucosa, and/or in the cortical region of the mesenteric lymph nodes, in 32 of the 52 cattle examined. Tissue sections from these animals were subjected to Ziehl-Neelsen staining, but the presence of acid-fast bacilli was not observed. Subsequent analysis, employing genus specific immunohistochemisty for Mycobacterium, revealed areas of immunoreactivity in sections prepared from a total of six animals. The results of this investigation highlighted the value of histopathology and particularly immunohistochemistry as tools for the diagnosis of subclinical paratuberculosis.
    Type of Medium: Online Resource
    ISSN: 0100-736X
    URL: Article
    DOI: 10.1590/S0100-736X2013001200006
    Language: Unknown
    Publisher: FapUNIFESP (SciELO)
    Publication Date: 2013
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  • 7
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    Aspectos clínico-patológicos e controle da paratuberculose em rebanho bovino leiteiro
    FapUNIFESP (SciELO) ; 2010
    In:  Pesquisa Veterinária Brasileira Vol. 30, No. 11 ( 2010-11), p. 921-932
    Details
    In: Pesquisa Veterinária Brasileira, FapUNIFESP (SciELO), Vol. 30, No. 11 ( 2010-11), p. 921-932
    Abstract: Paratuberculosis (Johne's disease) is a granulomatous enteritis of ruminants caused by Mycobacterium avium subsp. paratuberculosis. Epidemiology, clinic-pathological and laboratorial aspects of paratuberculosis in a dairy cattle herd are described. The disease was diagnosed from 2006 to 2009 in eight cows that presented chronic-intermittent diarrhea and chronic weight loss, in the Rio Claro municipality, Rio de Janeiro. At necropsy, the subserosal lymphatic vessels were proeminent and dilated, mesenteric nodes were enlarged and intestinal mucosa was corrugated, thickened and of microgranular aspect. From duodenum to the rectum, histopathology revealed severe and diffuse granulomatous inflammation of the lamina propria and submucosa, broadened and distorted villi, dilatation of the lymphatic vessels in their apex, lymphangioectasia and granulomatous lymphangitis in the submucosa. Ziehl-Neelsen stain showed variable amounts of acid-fast bacilli in macrophages, in Langhans giant cells and freely in the mucosa and submucosa of the small intestine, colon and lymphnodes. In some cows, the lamina propria presented severe hypertrophy, mainly in the jejunum and ileum. Mycobacterium avium subsp. paratuberculosis was isolated through bacterial cultivation of samples taken from feces, intestinal mucosa and milk, and identified through IS900 PCR. From 298 cows older than three years, the percentage of reactive animals was 40% by indirect ELISA test. The diagnosis of paratuberculosis was based on clinic-epidemiological data, serology, bacterial isolation in Herrold egg yolk medium with micobactin and on IS900 PCR. After the adoption of control measures, as slaughter of cows with clinical signs, selective slaughter of seropositive cows, removal of the calf from the dam at birth, and use of the colostrum bank, we observed a reduction from six clinical cases to only one case per year, in the last three years of the study.
    Type of Medium: Online Resource
    ISSN: 0100-736X
    URL: Article
    DOI: 10.1590/S0100-736X2010001100005
    Language: Unknown
    Publisher: FapUNIFESP (SciELO)
    Publication Date: 2010
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  • 8
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    Intoxicação experimental com as folhas de Jatropha gossypifolia (Euphorbiaceae) em ovinos
    FapUNIFESP (SciELO) ; 2008
    In:  Pesquisa Veterinária Brasileira Vol. 28, No. 6 ( 2008-06), p. 275-278
    Details
    In: Pesquisa Veterinária Brasileira, FapUNIFESP (SciELO), Vol. 28, No. 6 ( 2008-06), p. 275-278
    Abstract: Fresh green leaves of Jatropha gossypifolia (Euphorbiaceae) were lethal for sheep in single administrations of 40g/kg. The dose of 5g/kg did not cause poisoning, but intermediate doses caused death in part of the animals. The clinical course of poisoning was 6 to 22 days. The clinic and pathological picture in the experimental sheep was characterized by digestive, lung and heart disturbances, and also by slight microscopic liver and renal regressive alterations. These findings are similar to those observed in experiments with the seeds of Jatropha curcas in goats, sheep and calves, and with the fruits and leaves of Jatropha glauca and Jatropha aceroides in goats, performed by other authors. A comparison indicates that, independently of the plant species, the leaves of Jatropha spp. contain toxic compounds similar to those found in the seeds.
    Type of Medium: Online Resource
    ISSN: 0100-736X
    URL: Article
    DOI: 10.1590/S0100-736X2008000600003
    Language: Unknown
    Publisher: FapUNIFESP (SciELO)
    Publication Date: 2008
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  • 9
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    Transplacental transmission of Neospora caninum in naturally infected small ruminants from northeastern Brazil
    FapUNIFESP (SciELO) ; 2017
    In:  Pesquisa Veterinária Brasileira Vol. 37, No. 9 ( 2017-09), p. 921-925
    Details
    In: Pesquisa Veterinária Brasileira, FapUNIFESP (SciELO), Vol. 37, No. 9 ( 2017-09), p. 921-925
    Abstract: RESUMO: Toxoplasma gondii e Neospora caninum são reconhecidos como protozoário causadores de aborto em ovinos e caprinos. Desta forma, objetivou-se descrever a transmissão transplacentária desses agentes em pequenos ruminantes na região Nordeste do Brasil. Foram examinados seis fetos caprinos e onze fetos ovinos, totalizando 78 amostras de diferentes tecidos (cérebro, fígado, pulmão, rim e coração) provenientes de propriedades rurais com histórico de aborto. As amostras foram analisadas por Reação em Cadeia da Polimerase (PCR), Histopatologia (HP) e Imunohistoquímica (IHQ), com a finalidade de associar o abortamento a T. gondii e/ou N. caninum. Nenhuma amostra foi positiva na PCR e IHQ para T. gondii. Algumas amostras de cérebro, fígado, pulmão, rim e coração de fetos de caprinos e ovinos foram positivas na PCR para N. caninum. Na histopatologia foi observado leve infiltrado mononuclear no fígado e necrose com calcificação no SNC de um caprino, associada à imunomarcação positiva para N. caninum na IHQ e PCR positiva. Os resultados confirmam a transmissão vertical de N. caninum em caprinos naturalmente infectados na região nordeste do Brasil.
    Type of Medium: Online Resource
    ISSN: 1678-5150 , 0100-736X
    URL: Article
    DOI: 10.1590/s0100-736x2017000900004
    Language: Unknown
    Publisher: FapUNIFESP (SciELO)
    Publication Date: 2017
    detail.hit.zdb_id: 2028762-8
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  • 10
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    Intoxicação acidental por monensina em ovinos no Estado do Rio de Janeiro
    FapUNIFESP (SciELO) ; 2009
    In:  Pesquisa Veterinária Brasileira Vol. 29, No. 9 ( 2009-09), p. 743-746
    Details
    In: Pesquisa Veterinária Brasileira, FapUNIFESP (SciELO), Vol. 29, No. 9 ( 2009-09), p. 743-746
    Abstract: An outbreak of monensin poisoning in sheep in the State of Rio de Janeiro is described. From 180 animals, eight died after they had been fed with ration containing the ionophore. The poisoning had a variable course and was clinically characterized by apathy, heart arrhythmia, myoglobinuria, incoordenation, incapacity of getting up, and sternal decubitus; one sheep aborted. The macroscopic lesions consisted of pale areas in the myocardium, hydroperitoneum, hydrothorax, and pulmonary edema. Histopathological examination revealed degenerative-necrotic alterations in heart and skeletal muscles. In the myocardium lesions were more severe and were characterized by multifocal necrosis with substitution of the myofibres by fibrous tissue and interstitial mononuclear infiltration. Proliferation of satellite cells and mononuclear inflammatory reaction in skeletal muscles were also verified. It seems that the exaggerated addition of sodic monensin, eventually associated with improper homogenization of monensin in the ration, was responsible for the excessive ingestion of monensin by some animals.
    Type of Medium: Online Resource
    ISSN: 0100-736X
    URL: Article
    DOI: 10.1590/S0100-736X2009000900011
    Language: Unknown
    Publisher: FapUNIFESP (SciELO)
    Publication Date: 2009
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