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  • 1
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: Rat brain creatine kinase (CKB) gene expression is highest in the brain but is also detectable at lower levels in some other tissues. In the brain, the CKB enzyme is thought to be involved in the regeneration of ATP necessary for transport of ions and neurotransmitters. To understand the molecular events that lead to high CKB expression in the brain, we have determined the steady-state levels of CKB mRNA in homogeneous cultures of primary rat brain astrocytes, oligodendrocytes, and neurons. Northern blot analysis showed that whereas the 1.4-kb CKB mRNA was detectable in neurons, the level was about 17-fold higher in oligodendrocytes and 15-fold higher in astrocytes. The blots were hybridized with a CKB-specific 32P-antisense RNA probe, complementary to the 3’untranslated sequence of CKB, which hybridizes to CKB mRNA but not CKM mRNA. Also, the 5’and 3’ends of CKB mRNA from the glial cells were mapped, using exon-specific antisense probes in the RNase-protection assay, and were found to be the same in astrocytes and oligodendrocytes. This indicated that (a) the site of in vivo transcription initiation in astrocytes and oligodendrocytes was directed exclusively by the downstream, nonconcensus TTAA sequence at -25 bp in the CKB promoter that is also utilized by all other cell types that express CKB and (b) the 3’end of mature CKB mRNA was the same in astrocytes and oligodendrocytes. In addition, there was no detectable alternate splicing in exon 1, 2, or 8 of CKB mRNA in rat astrocytes and oligodendrocytes. Also, our studies showed that 1.4-kb CKB mRNA is expressed in established C6 glioma cells at an intermediate level about threefold higher than that in primary neurons.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Mitigation and adaptation strategies for global change 5 (2000), S. 51-60 
    ISSN: 1573-1596
    Keywords: carbon accounting ; carbon sequestration ; carbon sinks ; carbon storage ; equivalence time ; equivalence factor ; permanence ; tonne.year
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: Abstract Concern about the issue of permanence andreversibility of the effects of carbon sequestrationhas led to the need to devise accounting methods thatquantify the temporal value of storing carbon that hasbeen actively sequestered or removed from theatmosphere, as compared to carbon stored as a resultof activities taken to avoid emissions. This paperdescribes a method for accounting for the atmosphericeffects of sequestration-based land-use projects inrelation to the duration of carbon storage. Firstly,the time period over which sequestered carbon shouldbe stored in order to counteract the radiative forcingeffect of carbon emissions was calculated, based onthe residence time and decay pattern of atmosphericCO2, its Absolute Global Warming Potential. Thistime period was called the equivalence time, andwas calculated to be approximately 55 years. From thisequivalence time, the effect of storage of 1 tCO2 for 1 year was derived, and found to besimilar to preventing the effect of the emission of0.0182 tCO2. Potential applications of thistonne.year figure, here called the equivalencefactor, are then discussed in relation to theestimation of atmospheric benefits over time ofsequestration-based land use projects.
    Type of Medium: Electronic Resource
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  • 3
    Publication Date: 2022-03-08
    Description: 3rd Report prepared by The World in 2050 initiative
    Type: Report , NonPeerReviewed
    Format: text
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