Note: Cover -- Half Title -- Title Page -- Copyright Page -- Preface to the Second Edition -- Preface to the First Edition -- Contents -- 1. INTRODUCTION -- I. Breadth of Enzymology -- II. Brief History of Enzymology -- III. General Characteristics of Enzymes -- IV. Importance of Enzymology to Food Science, Nutritional, and Health Sciences -- V. Literature of Enzymology -- Review Questions -- References -- 2. THE PROTEIN NATURE OF ENZYMES -- I. Role of Proteins in Organisms -- II. Primary Structure of Proteins -- III. Secondary Structure of Proteins -- IV. Tertiary Structure of Proteins -- V. Solvation of Proteins -- VI. Quaternary Structure of Proteins -- VII. Multimolecular Organization of Enzymes -- Review Questions -- References -- 3. ENZYME PURIFICATION -- I. Need for Purification -- II. Selection of Starting Material -- III. Preliminary Purification -- IV. Purification -- V. Order of Application of Purification Steps -- VI. Quantitation of Purification Steps -- VII. Criteria of Purity -- VIII. Loss of Enzyme Activity During Purification -- Review Questions -- References -- 4. ACTIVE SITES AND FACTORS RESPONSIBLE FOR ENZYME CATALYSIS -- I. Active Sites of Enzymes -- II. Factors Responsible for Efficiency of Enzyme-Catalyzed Reactions -- Review Questions -- References -- 5. RATES OF REACTIONS -- I. Writing Rate Expressions -- II. Rate Laws -- III. Molecularity Versus Order of Reaction -- IV. Rates of Enzyme-Catalyzed Reactions -- Review Questions -- References -- 6. EFFECT OF SUBSTRATE CONCENTRATION ON RATES OF ENZYME-CATALYZED REACTIONS -- I. General Concept of Enzyme-Substrate Combination -- II. One-Substrate Reactions -- III. Two-Substrate Reactions -- IV. Substrate-Induced "Abnormal" Behavior -- Review Questions -- References -- 7. EFFECT OF ENZYME CONCENTRATION ON RATES OF ENZYME-CATALYZED REACTIONS. , I. Enzyme Concentration-Velocity Relationships -- II. Perturbing Factors in Enzyme Concentration-Velocity Relationships -- III. Active Enzyme Concentration -- Review Questions -- References -- 8. KINETIC CONSEQUENCES OF ENZYME INHIBITION -- I. Types of Inhibitors -- II. Linear Inhibition Patterns -- III. Sigmoidal Behavior as a Result of Inhibition -- IV. Interpretation of Results -- Review Questions -- References -- 9. ENZYME INHIBITORS -- I. Importance of Enzyme Inhibitors -- II. Mode of Action of Inhibitors -- III. Some Factors That Influence Degree of Inhibition -- IV. Specific Inhibitors -- V. Activation of Enzymes by Compounds Usually Considered to Be Inhibitors -- VI. Inhibition and Stimulation of Enzyme Activity by Gases -- Review Questions -- References -- 10. EFFECT OF pH ON RATES OF ENZYME-CATALYZED REACTIONS -- I. Effect of pH on Stability of Enzymes -- II. Effect of pH on Catalytic Activity of Enzymes -- Review Questions -- References -- 11. EFFECT OF TEMPERATURE ON RATES OF ENZYME-CATALYZED REACTIONS -- I. Parameters Affected by Temperature Changes -- II. Stability of Enzymes -- III. Changes in Solubility of Gases -- IV. Effect of Temperature on Equilibria -- V. Effect of Temperature on Transformation of Enzyme-Substrate Complex to Products -- VI. Quantitation of Thermal Denaturation of Enzymes -- VII. Conclusions -- Review Questions -- References -- 12. ENZYME COFACTORS -- I. General Nature of Cofactors -- II. Coenzymes in Enzyme-Catalyzed Reactions -- III. Prosthetic Groups in Enzyme-Catalyzed Reactions -- IV. Role of Some Other Organic Compounds -- V. Inorganic Ions in Enzyme-Catalyzed Reactions -- VI. Apoenzyme-Cofactor Associations -- VII. Role of Protein in Enzymes That Contain Cofactors -- Review Questions -- References -- 13. CLASSIFICATION AND NOMENCLATURE OF ENZYMES. , I. Bases for Naming of Enzymes and Problems with Nomenclature -- II. Commission on Enzymes -- III. Recommended Classification of Enzymes -- Review Questions -- References -- 14. INTRODUCTION TO THE HYDROLASES -- References -- 15. THE GLYCOSIDE HYDROLASES -- I. Specificity -- II. The Amylases -- III. Dextransucrase -- IV. Cellulase -- V. ß-Fructofuranosidase -- VI. ß-Galactosidase -- Review Questions -- References -- 16. PECTIC ENZYMES -- I. Pectinesterase -- II. The Polygalacturonases -- III. Pectate Lyases -- Review Questions -- References -- 17. THE ESTERASES -- I. Carboxylic Ester Hydrolases -- II. The Phosphoric Hydrolases -- Review Questions -- References -- 18. THE NUCLEASES AND BIOTECHNOLOGY -- I. General Degradation of Nucleic Acids in the Gastrointestinal Tract -- II. Recombinant DNA Technology -- III. Other Nucleases -- Review Questions -- References -- 19. THE PROTEOLYTIC ENZYMES -- I. Specificity Requirements -- II. Subdivision of the Proteolytic Enzymes -- III. The Serine Proteases -- IV. The Sulfhydryl Proteases -- V. Metal-Containing Proteolytic Enzymes -- VI. The Aspartic Proteases -- VII. Assay of Proteolytic Activity -- Review Questions -- References -- 20. ORDINARY AND LIMITED PROTEOLYSIS -- I. Digestion of Proteins -- II. Limited Proteolysis -- Review Questions -- References -- 21. INTRODUCTION TO THE OXIDOREDUCTASES -- References -- 22. LACTATE DEHYDROGENASE -- I. Reaction Catalyzed -- II. Substrate Specificity -- III. Coenzyme Specificity -- IV. Lactate Dehydrogenase as a Protein -- V. Biological Significance of Multiple Molecular Forms -- VI. Mechanism of Action -- Review Questions -- References -- 23. GLUCOSE OXIDASE -- I. Specificity -- II. Mechanism of Action -- III. Effect of pH -- IV Assay of Glucose Oxidase Activity -- Review Questions -- References -- 24. POLYPHENOL OXIDASE -- I. Reactions Catalyzed. , II. Assay of Hydroxylation Reaction -- III. Mechanism of Hydroxylation Reaction -- IV. Oxidation of o-Diphenols (Dehydrogenation) -- V. Mechanisms of Oxidation of Mono- and o-Diphenols -- VI. Reaction Inactivation of Polyphenol Oxidase -- VII. Assay of Activity on o-Diphenols -- VIII. Substrate Specificity -- IX. Multiple Molecular Forms -- X. Inhibition of Polyphenol Oxidase Activity -- XI. Protein Nature of Enzyme -- Review Questions -- References -- 25. XANTHINE OXIDASE -- I. Reactions Catalyzed -- Substrate Specifity -- II. Protein and Active Site Properties -- III. Involvement of FAD in Active Site -- IV. Kinetics of Reaction -- V. Mechanism -- VI. Electron Paramagnetic Resonance Spectrometry and Mechanism of Action -- VII. Enzyme Inhibition -- VIII. Assay of Activity -- Review Questions -- References -- 26. CATALASE AND PEROXIDASE -- I. Peroxidase -- II. Catalase -- Review Questions -- References -- 27. LIPOXYGENASE (LIPOXIDASE) -- I. pH-Activity Profile -- II. Specificity of Lipoxygenase -- III. Kinetics of Lipoxygenase-Catalyzed Reactions -- IV. Pathway and Mechanism of Action -- V. Enzyme Inhibition -- VI. Assay of Lipoxygenase Activity -- Review Questions -- References -- Index.