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  • 1
    Digitale Medien
    Digitale Medien
    Expression of sulphonylurea receptors in bovine monocytes from animals with a different metabolic rate (1997)
    Springer
    Pflügers Archiv 434 (1997), S. 712-720 
    Details
    ISSN: 1432-2013
    Schlagwort(e): Key words Metabolic rate ; Skeletal muscle cells ; Smooth muscle cells ; Monocytes ; Sulphonylurea receptor ; Flow cytometry
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract  The aim of this study was to investigate the relationship between expression of sulphonylurea receptors (SUR) and metabolic rate (MR). SUR on monocytes and cells from muscle tissue were detected using fluorescent glibenclamide and flow cytometry. Transmembrane potential differences were detected by oxonol dye fluorescence measurements. A bovine model was used to induce differences in the MR by exposure to different ambient temperatures (4°C and 18°C), by different feeding levels (1.0- and 1.6-fold the metabolizable energy requirement for maintenance) and by α2-adrenergic stimulation. We found that cells from skeletal muscle (m. semimembranosus), immunochemically identified as smooth muscle cells, skeletal muscle fibres and monocytes, responded in comparable fashions to glibenclamide and ATP, i.e. with a depolarization, and to cromakalim with a polarization, suggesting that monocytes are useful indicators of regulatory events occurring in muscle cells. Glibenclamide fluorescence was assumed to represent SUR associated with KATP channels. Significant differences were detected in the percentage of depolarized monocytes in the different variants of the model. A linear correlation between monocytes that bound fluorescent glibenclamide and the MR was evident (with a coefficient of determination of 0.94) and was reproducible following reduction of the MR, by α2-adrenergic stimulation, suggesting that expression is involved in the regulation of whole-body energy expenditure.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s004240050456
    Standort Signatur Einschränkungen Verfügbarkeit
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    Artikel (Nationallizenzen)
    Volltext
  • 2
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    Unbekannt
    Baltic observatory for oceanographic monitoring (BOOM) – a versatile test bed based on deep-sea standards (2007)
    In:  [Poster] In: EGU General Assembly 2007, 15.-20.04.2007, Vienna, Austria .
    Details
    Publikationsdatum: 2012-06-18
    Materialart: Conference or Workshop Item , NonPeerReviewed
    Standort Signatur Einschränkungen Verfügbarkeit
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    OceanRep: Poster
  • 3
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    Unbekannt
    Efficiency of benthic filter: Biological control of the emission of dissolved methane from sediments containing shallow gas hydrates at Hydrate Ridge (2006)
    AGU (American Geophysical Union)
    In:  Global Biogeochemical Cycles, 20 (GB2019).
    Details
    Publikationsdatum: 2018-03-16
    Beschreibung: In marine sedimentary environments, microbial methanotrophy represents an important sink for methane before it leaves the seafloor and enters the water column. Using benthic observatories in conjunction with numerical modeling of pore water gradients, we investigated seabed methane emission rates at cold seep sites with underlying gas hydrates at Hydrate Ridge, Cascadia margin. Measurements were conducted at three characteristic sites which have variable fluid flow and sulfide flux and sustain distinct chemosynthetic communities. In sediments covered with microbial mats of Beggiatoa, seabed methane efflux ranges from 1.9 to 11.5 mmol m−2 d−1. At these sites of relatively high advective flow, total oxygen uptake was very fast, yielding rates of up to 53.4 mmol m−2 d−1. In sediments populated by colonies with clams of the genus Calyptogena and characterized by low advective flow, seabed methane emission was 0.6 mmol m−2 d−1, whereas average total oxygen uptake amounted to only 3.7 mmol m−2 d−1. The efficiency of methane consumption at microbial mat and clam field sites was 66 and 83%, respectively. Our measurements indicate a high potential capacity of aerobic methane oxidation in the benthic boundary layer. This layer potentially restrains seabed methane emission when anaerobic methane oxidation in the sediment becomes saturated or when methane is bypassing the sediment matrix along fractures and channels.
    Materialart: Article , PeerReviewed
    Format: text
    DOI: 10.1029/2004GB002389
    Standort Signatur Einschränkungen Verfügbarkeit
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    OceanRep: Artikel in einer Fachzeitschrift - begutachtet
  • 4
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    Modulation of vH+-ATPase is part of the functional adaptation of sheep rumen epithelium to high-energy diet (2012)
    The American Physiological Society (APS)
    Details
    Publikationsdatum: 2012-11-02
    Beschreibung: Ruminal vacuolar H + -ATPase (vH + -ATPase) activity is regulated by metabolic signals. Thus, we tested whether its localization, expression, and activity were changed by different feeding. Young male sheep ( n = 12) were either fed hay ad libitum (h) or hay ad libitum plus additional concentrate (h/c) for 2 wk. The vH + -ATPase B subunit signal was predominantly found in the cell membrane and cytosol of rumen epithelial cells (REC) with basal/parabasal phenotype. The elevated number (threefold) of these cells in rumen mucosa of h/c-fed sheep reflects a high proliferative capacity and, explains the 2.3-fold increase of the total number of vH + -ATPase-expressing REC. However, in accordance with a 58% reduction of the vH + -ATPase B subunit mRNA expression in h/c-fed sheep, its protein amount per single REC was decreased. Using the fluorescent probe BCECF and selective inhibitors (foliomycin, amiloride), the contribution of vH + -ATPase and Na + /H + exchanger to intracellular pH (pH i ) regulation was investigated. REC isolated from h/c-fed sheep keep their pH i at a significantly higher level (6.91 ± 0.03 vs. 6.74 ± 0.05 in h-fed sheep). Foliomycin or amiloride decreased pH i by 0.16 ± 0.02 and 0.57 ± 0.04 pH units when applied to REC from h-fed sheep, but the effects were markedly reduced (–88 and –33%) after concentrate feeding. Nevertheless, we found that REC proliferation rate and [cAMP] i were reduced after foliomycin-induced vH + -ATPase inhibition. Our results provide the first evidence for a role of vH + -ATPase in regulation of REC proliferation, most probably by linking metabolically induced pH i changes to signaling pathways regulating this process.
    Print ISSN: 0363-6119
    Digitale ISSN: 1522-1490
    Thema: Medizin
    Publiziert von The American Physiological Society (APS)
    Standort Signatur Einschränkungen Verfügbarkeit
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