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  • 1
    Online Resource
    Online Resource
    San Diego :Elsevier Science & Technology,
    Keywords: Molecular biology. ; Electronic books.
    Type of Medium: Online Resource
    Pages: 1 online resource (768 pages)
    Edition: 1st ed.
    ISBN: 9780323142632
    Language: English
    Note: Front Cover -- Selected Papers in Molecular Biology by Jacques Monod -- Copyright Page -- Table of Contents -- Preface -- Chapter 1. Le taux de croissance en fonction de la concentration de l'aliment dans une population de Glaucoma piriformis en culture pure. -- Chapter 2. Ration d'entretien et ration de croissance dans les populations bactériennes. -- Chapter 3. Croissance des populations bactériennes en fonction de la concentration de Valiment hydrocarboné. -- Chapter 4. Sur un phénomène nouveau de croissance complexe dans les cultures bactériennes. -- Chapter 5. DIAUXIE ET RESPIRATION AU COURS DE LA CROISSANCE DES CULTURES DE B. COLI -- Chapter 6. SUR L'EXPRESSION ANALYTIQUE DE LA CROISSANCE DES POPULATIONS BACTÉRIENNES -- TRAVAUX CITÉS -- Chapter 7. INHIBITION DE L'ADAPTATION ENZYMATIQUE CHEZ B. COLI EN PRÉSENCE DE 2-4 DINITROPHÉNOL -- Chapter 8. SUR LA NATURE DU PHÉNOMÈNE DE DIAUXIE -- Chapter 9. MUTATION ET ADAPTATION ENZYMATIQUE CHEZ ESCHERICHIA COLI-MUTABILE -- INTRODUCTION -- TECHNIQUES EMPLOYÉES -- RÉSULTATS EXPÉRIMENTAUX -- DISCUSSION. -- RÉSUMÉ ET CONCLUSIONS. -- TRAVAUX CITES -- Chapter 10. SUR UNE MUTATION SPONTANÉE AFFECTANT LE POUVOIR DE SYNTHÈSE DE LA METHIONINE CHEZ UNE BACTÉRIE COLIFORME -- Introduction -- Techniques employées -- Résultats expérimentaux -- Discussion -- Conclusions -- TRAVAUX CITES -- Chapter 11. L'INHIBITION DE LA CROISSANCE ET DE L'ADAPTATION ENZYMATIQUE CHEZ LES BACTÉRIES INFECTÉES PAR LE BACTÉRIOPHAGE -- Introduction -- Souches et techniques employées -- Résultats expérimentaux. -- Discussion des résultats. -- Conclusions. -- BIRLIOGRAPHIE. -- Chapter 12. THE PHENOMENON OF ENZYMATIC ADAPTATION -- I. INTRODUCTION -- II. THE OCCURRENCE OF SUBSTRATE-INDUCED ENZYME FORMATION -- III. SUBSTRATE ACTION AND INTERACTIONS IN ENZYMATIC ADAPTATION -- IV. ADAPTIVE ENZYMES AND GENES. , V. THE ESSENTIAL FACTORS OF ADAPTIVE ENZYME SYNTHESIS -- VI. ADAPTIVE ENZYMES AND CELLULAR DIFFERENTIATION -- REFERENCES -- Chapter 13. Synthèse d'un polysaccharide du type amidon aux dépens du maltose, en présence d'un extrait enzymatique d'origine bactérienne -- Chapter 14. Sur une lactase extraite d'une souche d'Escherichia coli mutabile. -- Chapter 15. THE GROWTH OF BACTERIAL CULTURES -- INTRODUCTION -- DEFINITION OF GROWTH PHASES AND GROWTH CONSTANTS -- ON TECHNIQUES -- THE PHYSIOLOGICAL SIGNIFICANCE OF THE GROWTH CONSTANT -- CONCLUDING REMARKS -- LITERATURE CITED -- Chapter 16. DE L'AMYLOMALTASE D'ESCHERICHIA COLI -- INTRODUCTION -- MATÉRIEL ET TECHNIQUES. -- RÉSULTATS -- DISCUSSION -- RÉSUMÉ ET CONCLUSIONS. -- BIBLIOGRAPHIE -- Chapter 17. ADAPTATION, MUTATION AND SEGREGATION IN THE FORMATION OF BACTERIAL ENZYMES -- I -- II -- Ill -- IV -- REFERENCES -- Chapter 18. LA TECHNIQUE DE CULTURE CONTINUE THÉORIE ET APPLICATIONS -- I. - INTRODUCTION. -- II. - THÉORIE. -- III. - RÉALISATION. -- IV. - CONCLUSIONS ET COMMENTAIRES. -- BIBLIOGRAPHIE -- Chapter 19. SUR LA BIOSYNTHESE DE LA β-GALACTOSIDASE (LACTASE) CHEZ ESCHERICHIA COLI. LA SPECIFICITE DE L'INDUCTION* -- I. INTRODUCTION -- II. MATERIELS ET TECHNIQUES -- III. OBSERVATIONS EXPERIMENTALES -- IV. CONCLUSIONS -- REMERCIEMENTS -- RÉSUMÉ -- SUMMARY -- ZUSAMMENFASSUNG -- BIBLIOGRAPHIE -- Chapter 20. LA BIOSYNTHÈSE INDUITE DES ENZYMES (ADAPTATION ENZYMATIQUE) -- Introduction -- I. La biosynthèse induite des enzymes dans le métabolisme cellulaire -- II. Cinétique de l a biosynthèse induite des enzymes -- III. Facteurs spécifiques et relations de spécificité dans la biosynthèse induite des enzymes -- Travaux cités -- Chapter 21. LA CINÉTIQUE DE LA BIOSYNTHÈSE DE LA β-GALACTOSIDASE CHEZ E. COLI CONSIDÉRÉE COMME FONCTION DE LA CROISSANCE* -- INTRODUCTION -- MATÉRIELS ET TECHNIQUES. , L'EFFET DE CARENCES SPÉCIFIQUES E N ACIDES AMINÉS SUR LA BIOSYNTHÈSE DE LA ß-GALACTOSIDASE -- LA SYNTHÈSE D E LA GALACTOSIDASE COMME FONCTION DE LA CROISSANCE -- CONCLUSIONS ET DISCUSSION -- RÉSUMÉ -- SUMMARY -- ZUSAMMENFASSUNG -- BIBLIOGRAPHIE -- Chapter 22. L'effet d'inhibition spécifique dans la biosynthèse de la tryptophane-desmase chez Aerobacter aerogenes (1). -- Chapter 23. L'effet inhibiteur spécifique de la methionine dans la formation de la methionine-synthase chez Escherichia coli. -- Chapter 24. Terminology of Enzyme Formation -- Chapter 25. STUDIES ON THE INDUCED SYNTHESIS OF β-GALACTOSIDASE IN ESCHERICHIA COLI: THE KINETICS AND MECHANISM OF SULFUR INCORPORATION* -- INTRODUCTION -- MATERIALS AND METHODS -- EXPERIMENTAL -- DISCUSSION -- SUMMARY -- RÉSUMÉ -- ZUSAMMENFASSUNG -- REFERENCES -- Chapter 26. Remarks on the Mechanism of Enzyme Induction* -- Introduction -- Present Status of Knowledge on Induced Enzyme Synthesis -- Thiogalactosides -- Induction Kinetics and the Preinduction Effect -- The "Y" Galactoside-Concentrating System -- Glucose Effect and "Cellular Memory -- Specific Inhibition of Induction -- Conclusions -- REFERENCES -- Chapter 27. LA GALACTOSIDE-PERMÉASE D'ESCHERICHIA COLI (*) -- INTRODUCTION -- PRODUITS, SOUCHES ET TECHNIQUES -- EXPÉRIENCES. -- DISCUSSION ET CONCLUSIONS. -- RÉSUMÉ. -- SUMMARY. -- BIBLIOGRAPHIE -- Chapter 28. BACTERIAL PERMEASES1 -- I. INTRODUCTION -- II. ACCUMULATION, CRYPTICITY, AND SELECTIVE PERMEABILITY -- III. GALACTOSIDE-PERMEASE -- IV. OTHER CARBOHYDRATE PERMEASES OF ESCHERICHIA COLI -- V. THE PERMEATION OF KREBS CYCLE INTERMEDIATES AND OTHER ORGANIC ACIDS IN PSEUDOMONAS AND AEROBACTER -- VI. AMINO ACID PERMEASES -- GENERAL DISCUSSION -- ADDENDUM -- REFERENCES -- Chapter 29. The Genetic Control and Cytoplasmic Expression of "Inducibility" in the Synthesis of β-galactosidase by E. Coli. , 1. Introduction -- 2. Materials and Methods -- 3. Genetic Structure of the "Lac" Region -- 4. B-Galactosidase Synthesis by Heteromerozygotes -- 5. Discussion and Conclusions -- REFERENCES -- Chapter 30. Sur la présence de protéines apparentées à la β-galactosidase chez certains mutants d'Escherichia coli. -- Chapter 31. ON THE ENZYMIC ÀCETYIATION OF ISOPROPYL-β-D-THIOGALACTOSIDE AMD ITS ASSOCIATION WITH GALACTOSIDE-PERMEASE -- BIBLIOGRAPHY. -- Chapter 32. L'opéron : groupe de gènes à expression coordonnée par un opérateur. -- Chapter 33. Synthèse constitutive de galactokinase consécutive au développement des bacteriophages λ chez Escherichia coli K 12. -- Chapter 34. Effets d'un analogue de Vuracile sur les propriétés d'une protéine enzymatique synthétisée en sa précence. -- Chapter 35. Biosynthèse induite d'une protéine génétiquement modifiée, ne présentant pas d'affinité pour l'inducteur. -- Chapter 36. On the Expression of a Structural Gene -- 1. Introduction -- 2. Materials and Methods -- 3. Kinetics of Gene Expression upon Transfer -- 4. Effects on Enzyme Synthesis of P Disintegration occurring in the Transferred Material -- 5. Discussion -- REFERENCES -- Chapter 37. Genetic Regulatory Mechanisms in the Synthesis of Proteins -- 1. Introduction -- 2. Inducible and Repressible Enzyme Systems -- 3. Regulator Genes -- 4. The Operator and the Operon -- 5. The Kinetics of Expression of Structural Genes, and the Nature of the Structural Message -- 6 . Conclusion -- REFERENCES -- Chapter 38. On the Regulation of Gene Activity -- INTRODUCTION -- GENERAL MODEL -- MESSENGER RNA AS THE PRIMARY GENE PRODUCT -- STRUCTURAL AND REGULATOR GENES -- OPERATORS -- THE OPERON AS THE POLARIZED UNIT OF TRANSCRIPTION -- REGULATION AT THE GENETIC LEVEL -- CONCLUSIONS -- ACKNOWLEDGMENTS -- REFERENCES -- DISCUSSION. , Chapter 39. General Conclusions: Teleonomic Mechanisms in Cellular Metabolism, Growth, and Differentiation -- I. INTRODUCTION -- II. REGULATORY MECHANISMS -- III. REGULATION AND DIFFERENTIATION IN HIGHER ORGANISMS -- REFERENCES -- DISCUSSION -- Chapter 40. Thiogalactoside Transacetylase* -- EXPERIMENTAL PROCEDURE -- DISCUSSION -- SUMMARY -- REFERENCES -- Chapter 41. Sur la nature du répresseur assurant L'immunité des bactéries lysogènes (1). -- Genetic Repression, Allosteric Inhibition, and Cellular Differentiation -- Introduction -- Regulation of Enzymatic Activity:Allosteric Effects -- Regulation of Protein Synthesis in Bacteria -- Negative Regulation Controlled by Specific Genetic Determinants -- Operators and Polygenic Operons -- Messenger RNA and Regulation at the Genetic Level -- The Repressor -- Genetic Regulation and Differentiation -- Model Circuits -- Conclusion -- ACKNOWLEDGMENTS -- REFERENCES -- Chapter 42. Allosteric Proteins and Cellular Control Systems -- 1. Introduction -- 2. Allosteric Proteins as Metabolic Regulators -- 3. Allosteric Effects as Conformational Alterations -- 4. General Discussion and Conclusions -- REFERENCES -- Chapter 43. ON THE REVERSIBILITY BY TREATMENT WITH UREA OF THE THERMAL INACTIVATION OF E. COLI ß-GALACTOSIDASE -- References -- Chapter 44. Non-inducible Mutants of the Regulator Gene in the "Lactose" System of Escherichia coli -- 1. Introduction -- 2. Materials and Methods -- 3. The System for Lactose Utilization in E. coli -- 4. Properties and Mapping of i8 Mutations -- 5. Functional Analysis of Mutant i818 -- 6. Lac+ Revertants of i8 Mutants -- 7. Temperature-sensitive Secondary Mutant s of the i818 Allele -- 8. Conclusions -- REFERENCES -- Chapter 45. THE EFFECT OF 5 'ADENYLIC ACID UPON THE ASSOCIATION BETWEEN BROMTHYMOL BLUE AND MUSCLE PHOSPHORYLASE b -- References. , Chapter 46. On the Nature of Allosteric Transitions : A Plausible Model.
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  • 2
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Biochemistry 13 (1974), S. 5543-5547 
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 52 (1988), S. 0 
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract The genome of Bordetella pertussis contains a strictly conserved 530 base-pair (bp) repeated sequence present in about 70 to 80 copies and accounting for approximately 1% of the bacterial genome. The repeated element, whose complete nucleotide sequence has been determined, is specific for B. pertussis DNA; it could be detected neither in closely related Bordetella strains nor in other bacterial or eukaryotic DNAs. The repeated sequence is not associated with the control of the expression of virulence determinants.
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 10 (1981), S. 0 
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Osney Mead, Oxford OX2 0EL, UK : Blackwell Scientific Publications
    Molecular microbiology 17 (1995), S. 0 
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The adenylate cyclase toxin (CyaA) of Bordetella pertussis is a 1706-residue protein composed of an amino-terminal adenylate cyclase (AC) domain linked to a 1300-residue channel-forming RTX (repeats in toxin) haemolysin. The toxin delivers its AC domain into a variety of eukaryotic cells and impairs cellular functions by catalysing unregulated synthesis of cAMP from intracellular ATP. We have examined toxin activities of a set of deletion derivatives of CyaA. The results indicate that CyaA does not have a dedicated target cell-binding domain and that structural integrity and co-operation of all domains, as well as the post-translational fatty acylation mediated by an accessory protein CyaC, are all essential for target cell association and toxin activity of CyaA. When tested individually, all toxin derivatives were inactive and impaired in the tight association with the target cell surface. However, pairs of constructs with non-overlapping deletions complemented each other in vitro and exhibited a partially restored cytotoxic activity. This suggests that at least a part of the active toxin may act in the form of dimers or higher oligomers. The complementation analysis revealed that the last 217 residues of CyaA, containing the unprocessed secretion signal, form an autonomous domain essential for toxin activity, and that the region from residue 624 to 780 may be directly involved in delivery of the AC toxin into cells.
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  • 8
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: We report the Identification of a protein homologous to a histone H1 in Bordetella pertussis. The B. pertussis histone homologue, BpH1, varies in size in different strains from 182 to 206 amino acids. The variability of the size of the protein is due to gene variability by insertion or deletion of DNA modules. Insertion of a kanamycin cassette into the bpH1 gene generates a BpH1 null mutant with phenotypic properties and growth rate similar to those of the wild-type strain, showing that this gene is dispensable. In vitro, the BpH1 protein prevents chromosomal DNA degradation from DNase I and constrains supercoiled DNA. Transcription of the bpH1 gene is activated during exponential growth of the bacteria, whereas It is repressed during the stationary phase of growth, It is proposed that BpH1 plays a role in chromatin formation and condensation during DNA replication and that repression of transcription depends upon a reduced rate of DNA replication.
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  • 9
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: In wild-type Streptomyces coelicolor MT1110 cultures, cyclic adenosine 3′,5′ monophosphate (cAMP) was synthesized throughout the developmental programme with peaks of accumulation both during germination and later when aerial mycelium and actinorhodin were being produced. Construction and characterization of an adenylate cyclase disruption mutant (BZ1) demonstrated that cAMP facilitated these developmental processes. Although pulse-labelling experiments showed that a similar germination process was initiated in BZ1 and MT1110, germ-tube emergence was severely delayed in BZ1 and never occurred in more than 85% of the spores. Studies of growth and development on solid glucose minimal medium (SMMS, buffered or unbuffered) showed that MT1110 and BZ1 produced acid during the first rapid growth phase, which generated substrate mycelium. Thereafter, on unbuffered SMMS, only MT1110 resumed growth and produced aerial mycelium by switching to an alternative metabolism that neutralized its medium, probably by reincorporating and metabolizing extracellular acids. BZ1 was not able to neutralize its medium or produce aerial mycelium on unbuffered SMMS; these defects were suppressed by high concentrations (〉1 mM) of cAMP during early growth or on buffered medium. Other developmental mutants (bldA, bldB, bldC, bldD, bldG) also irreversibly acidified this medium. However, these bald mutants were not suppressed by exogenous cAMP or neutralizing buffer. BZ1 also differentiated when it was cultured in close proximity to MT1110, a property observed in cross-feeding experiments between bald mutants and commonly thought to reflect diffusion of a discrete positively acting signalling molecule. In this case, MT1110 generated a more neutral pH environment that allowed BZ1 to reinitiate growth and form aerial mycelium. The fact that actinorhodin synthesis could be induced by concentrations of cAMP (〈 20 μM) found in the medium of MT1110 cultures, suggested that it may serve as a diffusible signalling molecule to co-ordinate antibiotic biosynthesis.
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  • 10
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 7 (1993), S. 0 
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The cyaA gene of Bordetella pertussis and of Bordetella bronchiseptica encodes a toxin which is a bifunctional protein exhibiting adenylate cyclase and haemolytic activities. In Bordetella, virulence factors are synthesized under the control of the bvg regulatory locus, in response to environmental signals. In Escherichia coli the cyaA gene is not expressed, nor is it activated by bvg indicating that the activation of cya by bvg is indirect. To characterize cis-acting regulatory regions required for the activation of the cyaA gene we constructed cyaA–lacZY fusions containing progressive deletions in the promoter upstream region and isolated promoter mutations by chemical and site-directed mutagenesis. Deletion analysis shows that a region extending from −569 to −136 bp upstream from the start site of transcription is required for transactivation by bvg, suggesting that multiple binding sites are involved in the activation of the cyaA promoter. No single or double mutations in the promoter upstream region were found which conferred inactive or bvg-independent Cya phenotype. A double mutation in positions +10 and +13, relative to the transcription start site, rendered the promoter bvg-independent and functional in E. coli. The constitutive mutations create a new transcription start site, 20 bp downstream from the witd-type site, by providing new −10 and −35 elements recognized by RNA polymerase alone.
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