In:
Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, KSU Journal of Agriculture and Nature
Abstract:
The use of antibiotics, which have been used in the treatment of infections, has been increasing. Therefore, the occurance of antibiotic-resistant bacteria and resistance genes both in infections and in environmentalso increasing. In settlements near the water, wastewater is commonly collected in certain centers and discharged into nearby waters after treatment. As a result, the waters are polluted with pathogenic and resistant microorganisms, posing a great danger to human health. In this study, it was aimed to determine the antibiotic resistance profiles of Gram negative bacteria isolated from wastewater samples taken from Trabzon city center. Samples taken from wastewater were inoculated on various media to analyze the growing microorganisms. Thirty-six gram-negative bacteria were included in the study. Resistance in bacteria was investigated by disk diffusion method. Microorganisms with resistance were identified with MALDI-TOF MS, and resistance profiles were obtained with an automated system. The resistance gene in bacteria was investigated by PCR method and the transmission mechanisms of these genes were examined. In the current study, antibiotic resistance was found in 8 of 36 isolates. The presence of ESBL and blaoxa was shown to be in an isolate. It was determined that two isolates had plasmids. It was also shown that ampicillin resistance in one isolate was transformed with a conjugative plasmid, and kanamycin resistance was transformed with a non-conjugative plasmid. Detection of the presence of plasmid in some isolates carrying resistance suggests that antibiotic resistance may spread among bacteria in wastewater, and may have adverse effects on living things.
Type of Medium:
Online Resource
ISSN:
2619-9149
DOI:
10.18016/ksutarimdoga.vi.1248203
Language:
Turkish
Publisher:
KSU Journal of Agriculture and Nature
Publication Date:
2023
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