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  • 1
    Online Resource
    Online Resource
    Robust estimation of bacterial cell count from optical density
    Springer Science and Business Media LLC ; 2020
    In:  Communications Biology Vol. 3, No. 1 ( 2020-09-17)
    Details
    In: Communications Biology, Springer Science and Business Media LLC, Vol. 3, No. 1 ( 2020-09-17)
    Abstract: Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli . Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals   〈 1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
    Type of Medium: Online Resource
    ISSN: 2399-3642
    URL: Article
    DOI: 10.1038/s42003-020-01127-5
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2020
    detail.hit.zdb_id: 2919698-X
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  • 2
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    Author Correction: Robust estimation of bacterial cell count from optical density
    Springer Science and Business Media LLC ; 2020
    In:  Communications Biology Vol. 3, No. 1 ( 2020-10-27)
    Details
    In: Communications Biology, Springer Science and Business Media LLC, Vol. 3, No. 1 ( 2020-10-27)
    Abstract: An amendment to this paper has been published and can be accessed via a link at the top of the paper.
    Type of Medium: Online Resource
    ISSN: 2399-3642
    URL: Article
    DOI: 10.1038/s42003-020-01371-9
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2020
    detail.hit.zdb_id: 2919698-X
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  • 3
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    Retracted : Ras‐PI3K‐AKT signaling promotes the occurrence and development of uveal melanoma by downregulating H3K56ac expression
    Wiley ; 2019
    In:  Journal of Cellular Physiology Vol. 234, No. 9 ( 2019-09), p. 16032-16042
    Details
    In: Journal of Cellular Physiology, Wiley, Vol. 234, No. 9 ( 2019-09), p. 16032-16042
    Abstract: Uveal melanoma (UM) is an intraocular malignant tumor characterized by rapid progression and recurrence. The current conventional treatments are unsatisfactory. Histone acetylation at H3 lysine 56 (H3K56ac) has been reported to be a tumor suppressor in breast cancer. However, whether H3K56ac prevents the occurrence and development of UM remains uninvestigated. The study aimed to explore the regulatory effect of H3K56ac on Ras‐PI3K‐AKT induced UM cells proliferation and migration. Methods The vectors of pEGFP‐Ras WT , pEGFP‐K‐Ras G12V/Y40C , and pEGFP‐N1 were transfected into MP46 cells, and protein levels of phosphorylated AKT Ser473 and H3K56ac were examined using western blot analysis. The effect of H3K56ac on cell proliferation and migration were studied using 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5 diphenyl tetrazolium bromide, colony formation, and Transwell assays. Reverse transcription‐quantitative polymerase chain reaction (RT‐qPCR) and chromatin immunoprecipitation assays were performed to determine the phosphoinositide 3‐kinase/protein kinase B (PI3K/AKT) downstream genes. Further, the regulatory effects of silent mating type information regulation 2 homolog‐1 (SIRT1), general control nonderepressible 5 (GCN5), and mouse double minute 2 homolog (MDM2) on Ras‐PI3K‐AKT affected H3K56ac expression were also investigated. Results H3K56ac expression was specifically downregulated by Ras‐PI3K‐AKT activation pathway. H3K56ac inhibited the tumorigenic effect of Ras‐PI3K‐AKT on MP46 cells viability, colony formation, and migration, as well as participated in regulating the transcription of PI3K/AKT downstream genes. SIRT1 silence recovered H3K56ac expression, and reversed the tumorigenic effect of Ras‐PI3K‐AKT activation on MP46 cells. Downregulation of H3K56ac induced by Ras‐PI3K‐AKT activation was found to be associated with MDM2‐mediated the degradation of GCN5. Conclusions The results demonstrated that Ras‐PI3K‐AKT signaling promoted UM cells proliferation and migration via downregulation of H3K56ac expression, which might be related to MDM2‐mediated the degradation of GCN5.
    Type of Medium: Online Resource
    ISSN: 0021-9541 , 1097-4652
    URL: Issue
    URL: Article
    DOI: 10.1002/jcp.v234.9
    DOI: 10.1002/jcp.28261
    Language: English
    Publisher: Wiley
    Publication Date: 2019
    detail.hit.zdb_id: 1478143-8
    SSG: 12
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  • 4
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    Online Resource
    The advantage of praseodymium doped TiO2 composite as the support of Au catalysts for CO oxidation in CO2-rich atmosphere
    Elsevier BV ; 2023
    In:  Applied Catalysis A: General Vol. 661 ( 2023-07), p. 119261-
    Details
    In: Applied Catalysis A: General, Elsevier BV, Vol. 661 ( 2023-07), p. 119261-
    Type of Medium: Online Resource
    ISSN: 0926-860X
    URL: Article
    DOI: 10.1016/j.apcata.2023.119261
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2023
    detail.hit.zdb_id: 2024707-2
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  • 5
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    Effectiveness of therapeutic ultrasound for the treatment of carpal tunnel syndrome (the USTINCTS trial): study protocol for a three-arm, prospective, multicentre, randomised controlled trial
    BMJ ; 2022
    In:  BMJ Open Vol. 12, No. 4 ( 2022-04), p. e057541-
    Details
    In: BMJ Open, BMJ, Vol. 12, No. 4 ( 2022-04), p. e057541-
    Abstract: There has no consensus on optimal management of carpal tunnel syndrome (CTS), the most common compression neuropathy. Conservative therapy is generally accepted as first-line intervention. Therapeutic ultrasound has been widely reported to be treatment beneficial in nerve regeneration and conduction, and further accelerate compression recovery. The purpose of this study is to investigate the effectiveness of therapeutic ultrasound for CTS treatment. Methods and analysis This study protocol entails a three-arm, prospective, multicentre, randomised controlled trial. 162 eligible adult participants diagnosed with mild to moderate CTS by using criteria developed from a consensus survey by the UK Primary Care Rheumatology Society will be assigned to either (1) therapeutic ultrasound, (2) night splint or (3) therapeutic ultrasound +night splint (combined) group. Primary outcome will be difference in Symptom Severity Scale of Boston Carpal Tunnel Questionnaire (BCTQ-SSS) at 6-week between night splint and therapeutic ultrasound +night splint groups. Secondary outcomes include Functional Status Scale of BCTQ, sleep questionnaire for interrupted sleep, EuroQol-5D for general health, Hospital Anxiety and Depression Scale for mental status, Work Limitations Questionnaire-25 for functional limitations at work, Global Rating of Change for treatment success and recurrence rate, physical examination, electrophysiological and ultrasound parameters. Intention-to-treat analyses will be used. Ethics and dissemination Ethics committees of all clinical centres have approved this study. The leading centre is Shanghai Sixth People’s Hospital, whose approval number is 2021-152. New versions with appropriate amendments will be submitted to the committee for further approval. Final results will be published in peer-reviewed journals and presented at local, national and international conferences. Trial registration number ChiCTR2100050701.
    Type of Medium: Online Resource
    ISSN: 2044-6055 , 2044-6055
    URL: Article
    DOI: 10.1136/bmjopen-2021-057541
    Language: English
    Publisher: BMJ
    Publication Date: 2022
    detail.hit.zdb_id: 2599832-8
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  • 6
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    Online Resource
    Observation of Bloch oscillations dominated by effective anyonic particle statistics
    Springer Science and Business Media LLC ; 2022
    In:  Nature Communications Vol. 13, No. 1 ( 2022-05-02)
    Details
    In: Nature Communications, Springer Science and Business Media LLC, Vol. 13, No. 1 ( 2022-05-02)
    Abstract: Bloch oscillations are exotic phenomena describing the periodic motion of a wave packet subjected to an external force in a lattice, where a system possessing single or multiple particles could exhibit distinct oscillation behaviors. In particular, it has been pointed out that quantum statistics could dramatically affect the Bloch oscillation even in the absence of particle interactions, where the oscillation frequency of two pseudofermions with an anyonic statistical angle of $${{{\boldsymbol{\pi }}}}$$ π becomes half of that for two bosons. However, these statistically dependent Bloch oscillations have never been observed in experiments until now. Here, we report the experimental simulation of anyonic Bloch oscillations using electric circuits. By mapping the eigenstates of two anyons to the modes of the designed circuit simulators, the Bloch oscillations of two bosons and two pseudofermions are verified by measuring the voltage dynamics. The oscillation period in the two-boson simulator is almost twice of that in the two-pseudofermion simulator, that is consistent with the theoretical prediction. Our proposal provides a flexible platform to investigate and visualize many interesting phenomena related to particle statistics and could have potential applications in the field of the signal control.
    Type of Medium: Online Resource
    ISSN: 2041-1723
    URL: Article
    DOI: 10.1038/s41467-022-29895-0
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2022
    detail.hit.zdb_id: 2553671-0
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  • 7
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    Efficacy of ultrasound therapy for the treatment of lateral elbow tendinopathy (the UCICLET Trial): study protocol for a three-arm, prospective, multicentre, randomised controlled trial
    BMJ ; 2022
    In:  BMJ Open Vol. 12, No. 1 ( 2022-01), p. e057266-
    Details
    In: BMJ Open, BMJ, Vol. 12, No. 1 ( 2022-01), p. e057266-
    Abstract: Lateral elbow tendinopathy (LET) is a highly prevalent disease among the middle-aged population, with no consensus on optimal management. Non-operative treatment is generally accepted as the first-line intervention. Ultrasound (US) therapy has been reported to be beneficial for various orthopaedic diseases, including tendinopathy. The purpose of this study is to investigate the efficacy of US for LET treatment. Methods and analysis This protocol entails a three-arm, prospective, multicentre, randomised controlled trial. Seventy-two eligible participants with clinically confirmed LET will be assigned to either (1) US, (2) corticosteroid injections or (3) control group. All participants will receive exercise-based therapy as a fundamental intervention. The primary outcome is Patient-rated Tennis Elbow Evaluation. The secondary outcomes include Visual Analogue Scale for pain, shortened version of the Disabilities of the Arm, Shoulder and Hand for upper limb disability, pain free/maximum grip strength, Work Limitations Questionnaire-25 for functional limitations at work, EuroQol-5D for general health, Hospital Anxiety and Depression Scale for mental status, Global Rating of Change for treatment success and recurrence rate, and Mahomed Scale for the participant’s satisfaction. Adverse events will be recorded. Intention-to-treat analyses will be used. Ethics and dissemination Ethics committees of all clinical centres have approved this study. The leading centre is Shanghai Sixth People’s Hospital, whose approval number is 2021–153. New versions with appropriate amendments will be submitted to the committee for further approval. Final results will be published in peer-reviewed journals and presented at local, national and international conferences. Trial registration number ChiCTR2100050547.
    Type of Medium: Online Resource
    ISSN: 2044-6055 , 2044-6055
    URL: Article
    DOI: 10.1136/bmjopen-2021-057266
    Language: English
    Publisher: BMJ
    Publication Date: 2022
    detail.hit.zdb_id: 2599832-8
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  • 8
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    Online Resource
    Dual-engineered cartilage-targeting extracellular vesicles derived from mesenchymal stem cells enhance osteoarthritis treatment via miR-223/NLRP3/pyroptosis axis: Toward a precision therapy
    Elsevier BV ; 2023
    In:  Bioactive Materials Vol. 30 ( 2023-12), p. 169-183
    Details
    In: Bioactive Materials, Elsevier BV, Vol. 30 ( 2023-12), p. 169-183
    Type of Medium: Online Resource
    ISSN: 2452-199X
    URL: Article
    DOI: 10.1016/j.bioactmat.2023.06.012
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2023
    detail.hit.zdb_id: 2970496-0
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  • 9
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    Observation of novel topological states in hyperbolic lattices
    Springer Science and Business Media LLC ; 2022
    In:  Nature Communications Vol. 13, No. 1 ( 2022-05-26)
    Details
    In: Nature Communications, Springer Science and Business Media LLC, Vol. 13, No. 1 ( 2022-05-26)
    Abstract: The discovery of novel topological states has served as a major branch in physics and material sciences. To date, most of the established topological states have been employed in Euclidean systems. Recently, the experimental realization of the hyperbolic lattice, which is the regular tessellation in non-Euclidean space with a constant negative curvature, has attracted much attention. Here, we demonstrate both in theory and experiment that exotic topological states can exist in engineered hyperbolic lattices with unique properties compared to their Euclidean counterparts. Based on the extended Haldane model, the boundary-dominated first-order Chern edge state with a nontrivial real-space Chern number is achieved. Furthermore, we show that the fractal-like midgap higher-order zero modes appear in deformed hyperbolic lattices, and the number of zero modes increases exponentially with the lattice size. These novel topological states are observed in designed hyperbolic circuit networks by measuring site-resolved impedance responses and dynamics of voltage packets. Our findings suggest a useful platform to study topological phases beyond Euclidean space, and may have potential applications in the field of high-efficient topological devices, such as topological lasers, with enhanced edge responses.
    Type of Medium: Online Resource
    ISSN: 2041-1723
    URL: Article
    DOI: 10.1038/s41467-022-30631-x
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2022
    detail.hit.zdb_id: 2553671-0
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  • 10
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    Retracted : Ras–ERK1/2 signaling promotes the development of uveal melanoma by downregulating H3K14ac
    Wiley ; 2019
    In:  Journal of Cellular Physiology Vol. 234, No. 9 ( 2019-09), p. 16011-16020
    Details
    In: Journal of Cellular Physiology, Wiley, Vol. 234, No. 9 ( 2019-09), p. 16011-16020
    Abstract: Ras–extracellular signal‐regulated protein kinases 1 and 2 (ERK1/2) signaling has been proposed as the crucial regulators in the development of various cancers. Histone acetylation at H3 lysine 14 (H3K14ac) is closely associated with gene expression and DNA damage. However, whether H3K14ac participates in mediating Ras–ERK1/2‐induced cell proliferation and migration in uveal melanoma cells remains unknown. The purpose of this study is to investigate the effect of H3K14ac on Ras–ERK1/2 affected uveal melanoma cell phenotypes. MP65 cells were transfected with Ras WT and Ras G12V/T35S , the unloaded plasmid of pEGFP‐N1 served as a negative control. Protein levels of phosphorylated ERK1/2 Thr202 and H3K14ac were assessed by western blot assay. Cell viability, number of colonies, migration, and the downstream genes of ERK1/2 were analyzed by 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl‐2‐H‐tetrazolium bromide, soft‐agar colony formation, transwell, and chromatin immunoprecipitation assays. HA‐tag vectors of CLR3 and TIP60 and the small interfering RNAs that specific for CLR3 and MDM2 were transfected into MP65 cells to uncover the effects of CLR3, TIP60, and MDM2 on Ras–ERK1/2 mediated H3K14ac expression and MP65 cell phenotypes. We found that, Ras–ERK1/2 decreased H3K14ac expression in MP65 cells, and H3K14ac significantly suppressed Ras–ERK1/2‐induced cell viability, colony formation, and migration in MP65 cells. Moreover, the transcription of CYR61 , IGFBP3 , WNT16B , NT5E , GDF15 , and CARD16 was regulated by H3K14ac. Additionally, CLR3 silence recovered H3K14ac expression and reversed the effect of Ras–ERK1/2 on MP65 cell proliferation, migration and the mRNAs of ERK1/2 downstream genes. Besides, Ras–ERK1/2 decreased H3K14ac expression by MDM2‐mediated TIP60 degradation. In conclusion, Ras–ERK1/2 promoted uveal melanoma cells growth and migration by downregulating H3K14ac via MDM2‐mediated TIP60 degradation.
    Type of Medium: Online Resource
    ISSN: 0021-9541 , 1097-4652
    URL: Issue
    URL: Article
    DOI: 10.1002/jcp.v234.9
    DOI: 10.1002/jcp.28259
    Language: English
    Publisher: Wiley
    Publication Date: 2019
    detail.hit.zdb_id: 1478143-8
    SSG: 12
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