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Membrane-associated protein kinase activities in developing apple fruit (2003)

Duan, Chang-Qing ; Shen, Yuan-Yue ; Liang, Xiao-E ; [et al.]

Oxford, UK : Blackwell Science Ltd

Physiologia plantarum 118 (2003), S. 0

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ISSN: 1399-3054

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Biology

Notes: Fruit development is a process involving various signals and gene expression. Protein phosphorylation catalysed by protein kinases is known to play a key role in eukaryotic cell signalling and so may be involved in the regulation of fruit development. Using the method of exogenous substrate phosphorylation, the activity of calcium-dependent and calmodulin-independent protein kinase (CDPK) that was stimulated by phosphatidylserine, and the myelin basic protein (MBP)-phosphorylating activity that could be due to a calcium-independent mitogen-activated protein kinase-like (MAPK-like) activity in the developing apple fruits were identified. The CDPK activity was shown to be predominantly localized in the plasma membrane, whereas in the presence of phosphatidylserine, the high activity of CDPK was detected in both plasma membrane and endomembranes. The MAPK-like activity was predominantly associated with endomembranes. The assays of bivalent cation requirement showed that Mn2+ could replace Mg2+ in the incubation system for the protein kinase activities and stimulate CDPK activity more than Mg2+. Heat treatment abolished CDPK but stimulated MAPK-like activity. The activities of the phosphatidylserine-stimulated CDPK and of the MAPK-like were fruit developmental stage-specific with higher activities of both enzymes in the early and middle developmental stages in comparison with the late developmental stage. These data suggest that the detected protein kinases may play an important role in the fruit development.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1034/j.1399-3054.2003.00103.x

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The Mg-chelatase H subunit is an abscisic acid receptor (2006)

Shen, Yuan-Yue ; Wang, Xiao-Fang ; Wu, Fu-Qing ; [et al.]

[s.l.] : Nature Publishing Group

Nature 443 (2006), S. 823-826

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ISSN: 1476-4687

Source: Nature Archives 1869 - 2009

Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics

Notes: [Auszug] Abscisic acid (ABA) is a vital phytohormone that regulates mainly stomatal aperture and seed development, but ABA receptors involved in these processes have yet to be determined. We previously identified from broad bean an ABA-binding protein (ABAR) potentially involved in stomatal signalling, the ...