In:
Experimental Dermatology, Wiley, Vol. 31, No. 2 ( 2022-02), p. 191-201
Abstract:
Methylisothiazolinone (MI) and Methylchloroisothiazolinone (MCI) are among the most common skin sensitizers, yet the immunological events that occur during MCI/MI allergic contact dermatitis (ACD) are still poorly understood. Objectives: To analyse dendrocytes, macrophage subtypes and T cells in skin during the elicitation phase of MCI/MI ACD. Methods Thirteen patients with positive patch test reactions to MCI/MI (ACD group) and 11 individuals with negative patch test results were selected. Skin biopsies were only performed at 48 hours of patch testing. Immunohistochemistry was conducted to assess T cells, dendrocytes (Factor XIIIa), M1 (p‐Stat1, CD68) and M2 (c‐Maf, CD163) macrophages. Transcriptional analyses were performed for cytokines and related factors, and further compared to atopic dermatitis samples (n=4). Immunofluorescence assays addressed T cells location, along with IL‐4 or IL‐13, within the skin. Results MCI/MI elicited dermal dendrocytes and macrophages, pronouncedly the M2 subtype. T cells, majorly CD4+ T cells, accumulated in the perivascular areas. Similarly, abundant IL‐4 protein was detected in these areas. There was an upregulation of IL‐4 and IL‐13 mRNA expression, a mild increase in IFNG mRNA levels and a down‐regulation of RORC in the ACD group. Immunofluorescence revealed dermal clusters of T cells co‐localized with IL‐4. Conclusions M2 macrophages and Th2 cells participate in the immunopathogenesis of MCI/MI ACD. Dermal dendrocytes and M2 macrophages may assist the formation of CD4+ T cells perivascular clusters. These findings render a mechanistic insight into the MCI/MI reaction. Further analysis at different timepoints of patch testing is required to fully comprehend this ACD kinetics.
Type of Medium:
Online Resource
ISSN:
0906-6705
,
1600-0625
Language:
English
Publisher:
Wiley
Publication Date:
2022
detail.hit.zdb_id:
2026228-0
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