In:
The Journal of Immunology, The American Association of Immunologists, Vol. 198, No. 1_Supplement ( 2017-05-01), p. 201.30-201.30
Abstract:
We analyzed 18 RNA-seq libraries from 3 bovine calves at day 0, 7, 14 and 28, together with their dams (day 0 and 7) for cytokine gene expression. Expression of IL7, required for B- and T-cell development, significantly increases from day 0 to day 28 post-birth (LogFC 1.98, FDR 0.0054). By contrast IL7R gene expression significantly declined from day 0 to day 7 (LogFC −1.22, FDR 6.1E-08). The IL21 was expressed at low levels but IL21R gene expression increased day 0 vs day 7 (LogFC 1.17, FDR 0.000140). IL12B expression, a subunit of IL-12, increased from day 0 vs day 7 (LogFC1.14, FDR 0.016) and then declined. In parallel, IL12RB1 expression increased from day 0 to day 7 to achieve adult levels (LogFC1.31, FDR 5.5E-10). By contrast, IL12RB2 declined significantly within 7 days post-birth (LogFC −1.46, FDR 3.1E-05). Both IL12RB1 and IL12RB2 constitute IL12 receptor complex involved in T cell differentiation. The IL27 was expressed at low levels at birth but reached adult levels by day 7 (LogFC 1,66, FDR 0.006). The IL1B expression increased post-birth by day 7 (LogFC 1.44, FDR 0.0083) whereas IL1R2 gene was highly expressed at birth but declined by day 7 (LogFC −3.22, FDR 5.9E-34). Expression of chemokine IL8 that encodes CXCL8 declined day 0 vs day 7 post-birth (LogFC −1.13, FDR 0.00015). The pro-inflammatory cytokine TNFα gene expression increased by day 14 (LogFC 1.04, FDR 0.015). While various cytokines are expressed in a dynamic manner, an increase in IL-1B, IL-7, IL-12B and a decrease in IL-8 appears to be of functional significance in the development of neonatal immunity. These observations are in contrast to other species such as humans. [Supported by NSERC Discovery Grant]
Type of Medium:
Online Resource
ISSN:
0022-1767
,
1550-6606
DOI:
10.4049/jimmunol.198.Supp.201.30
Language:
English
Publisher:
The American Association of Immunologists
Publication Date:
2017
detail.hit.zdb_id:
1475085-5
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