ISSN:
0951-4198
Keywords:
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Physics
Notes:
The use 4-hydroxybenzenesulfonic acid as reduction-inhibiting and general purpose matrix in fast-atom bombardment liquid secondary-ion mass spectrometry (FAB/LSIMS) is discussed. Results obtained in a comparative study using the disulfide-bridge containing peptide deaminoarginine-vasopressin and several commonly used matrices indicate that the reduction-inhibitaing efficiency of 4-hydroxybenzenesulfonic acid is comparable to that of 3-nitrobenzyl alcohol while recuction is found to be important in matrices such as thioglycerol and glycerold. Experiments conducted on peptides such as met-enkephalin and bradykinin, nucleosides and other polar compounds reveal that 4-hydroxybenzenesulfonic acid is a good solvent and has physical properties which are similar to those of glycerol. This new matrix material is a radical scavenger, surface active, allows persistent secondary-ion beams and produces a low background signal. The matrix, 4-hydroxy-benzenesulfonic acid, generally yields high quality mass spectra which compare well with those obtained in either glycerol or 3-nitrobenzyl alcohol.
Additional Material:
3 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/rcm.1290051204
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