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  • 1
    Online Resource
    Online Resource
    Proteins of the canine seminal plasma
    FapUNIFESP (SciELO) ; 2016
    In:  Ciência Rural Vol. 46, No. 5 ( 2016-05), p. 901-908
    Details
    In: Ciência Rural, FapUNIFESP (SciELO), Vol. 46, No. 5 ( 2016-05), p. 901-908
    Abstract: RESUMO: Pesquisas têm sido realizadas para identificar as proteínas presentes no plasma seminal canino, com o intuito de relacioná-las com a qualidade espermática, bem como buscar por marcadores moleculares de patologias do trato reprodutivo. Há evidências de que as proteínas ligadoras de heparina, ligadoras de zinco, a lactoferrina, bem como as enzimas matrix metalloproteinase, superoxide dismutase, catalase e a glutationa peroxidase estão relacionadas com a qualidade seminal canina. Outras pesquisas indicam que a prolactina, e as enzimas arginina esterase, fosfatase ácida e fosfatase alcalina poderiam ser utilizadas com sucesso como biomarcadores de doenças reprodutivas. Assim, esta revisão de literatura objetiva abordar aspectos relacionados às proteínas do plasma seminal canino, suas influências sobre a fertilidade, e sua importância como biomarcadores de doenças reprodutivas.
    Type of Medium: Online Resource
    ISSN: 0103-8478
    URL: Article
    DOI: 10.1590/0103-8478cr20150972
    Language: Unknown
    Publisher: FapUNIFESP (SciELO)
    Publication Date: 2016
    detail.hit.zdb_id: 2025834-3
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  • 2
    Online Resource
    Online Resource
    Efeitos da Insulação Escrotal sobre a Biometria Testicular e Parâmetros Seminais em Carneiros da Raça Santa Inês Criados no Estado do Ceará
    FapUNIFESP (SciELO) ; 2001
    In:  Revista Brasileira de Zootecnia Vol. 30, No. 6 ( 2001-12), p. 1704-1711
    Details
    In: Revista Brasileira de Zootecnia, FapUNIFESP (SciELO), Vol. 30, No. 6 ( 2001-12), p. 1704-1711
    Abstract: A study was conducted to determine the effect of heat stress on semen criteria and testis size in Santa Inês hairy rams, in the State of Ceará, Northeast of Brazil. The scrotum of eight adult rams was insulated for seven days. Animals were evaluated twice before insulation and, after insulation, at 12 different periods until 118 days. Scrotal insulation did not affect semen volume, but pH was increased after eight days, returning to normal values after 15 days. Sperm concentration was reduced eight days after insulation was removed and animals became azzospermic between 33 and 50 days. After 79 days, sperm concentration returned to values similar to those observed before treatment. Scrotal circumference (26.4 cm) decreased to 22.4 cm eight days after insulation and to 21 cm at 21 days, but returned to 24.9 cm after 50 days. Moreover, sperm motility and vigor were reduced right after insulation was removed and returned to normal profiles only after 90 days. Before treatment, there were 1.7% of sperm cells with primary defects and 9.9% with secondary defects. Right after insulation, sperm cells with primary defects increased to 3.6% and those with secondary defects, to 43.4%, and after eight days, these values increased to 8.4 and 60.4%, respectively. From 15 to 60 days, sperm cells with primary defects varied from 27.3 to 16.8%, while those with secondary defects showed only small variations (39.9 to 39%). At the end of the experiment (118 days after insulation), primary defects were reduced to 0.7%, but the percentage of cells with secondary defects was still high (24.4 %). Therefore, heat stress caused temporary interruption of sperm production in the ram and sperm motility and secondary defects seemed to be the most sensitive criteria.
    Type of Medium: Online Resource
    ISSN: 1516-3598
    URL: Article
    DOI: 10.1590/S1516-35982001000700007
    Language: Unknown
    Publisher: FapUNIFESP (SciELO)
    Publication Date: 2001
    detail.hit.zdb_id: 2078814-9
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  • 3
    Online Resource
    Online Resource
    ÍNDICES DE TOLERÂNCIA AO CALOR DE VACAS LEITEIRAS NO PERÍODO CHUVOSO E SECO NO CEARÁ
    Pontificia Universidade Catolica do Parana - PUCPR ; 2012
    In:  Revista Acadêmica Ciência Animal Vol. 10, No. 4 ( 2012-10-15), p. 335-
    Details
    In: Revista Acadêmica Ciência Animal, Pontificia Universidade Catolica do Parana - PUCPR, Vol. 10, No. 4 ( 2012-10-15), p. 335-
    Abstract: O estudo avaliou a tolerância ao calor de um rebanho de fêmeas leiteiras mestiças (Bos taurus x Bos indicus) criadas em região litorânea do estado do Ceará. Foram avaliados os parâmetros ambientais para o cálculo do índice de temperatura e umidade (ITU) e a temperatura retal (TR) para o cálculo do índice de tolerância ao calor (ITC) durante o período chuvoso e seco do ano. Observou-se que os valores da TR encontravam-se dentro da normalidade quando se avaliou os dados médios em ambos os períodos. Os valores médios de temperatura ambiente (TA) estavam superiores à zona de conforto térmico para animais taurinos, com resultados bem próximos do limite máximo para mestiços e zebuínos quando se analisou os dados de máxima no turno da tarde do período seco. Os valores de umidade relativa do ar (UR) mostraram um comportamento inverso à temperatura ambiente, observando-se sempre os maiores valores no período chuvoso, bem como no turno da manhã quando comparado com o turno da tarde, em ambos os períodos. Os valores encontrados para o ITU mostraram condições de ambiente que passaram de críticos para emergenciais quando não se avaliaram somente os dados médios. Ambos os índices de tolerância ao calor mostraram uma menor tolerância ao calor dos animais no período seco do ano. Conclui-se que os animais sofrem com o calor em ambos os períodos, sendo o período seco menos favorável, em que foram verificados valores mais elevados de TR, TA, ITU e menores de ITC.
    Type of Medium: Online Resource
    ISSN: 2596-2868
    URL: Article
    DOI: 10.7213/academica.7739
    Language: Unknown
    Publisher: Pontificia Universidade Catolica do Parana - PUCPR
    Publication Date: 2012
    detail.hit.zdb_id: 3007483-6
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  • 4
    Online Resource
    Online Resource
    Desenvolvimento testicular, espermatogênese e concentrações hormonais em touros Angus
    FapUNIFESP (SciELO) ; 2006
    In:  Revista Brasileira de Zootecnia Vol. 35, No. 4 suppl ( 2006-08), p. 1629-1638
    Details
    In: Revista Brasileira de Zootecnia, FapUNIFESP (SciELO), Vol. 35, No. 4 suppl ( 2006-08), p. 1629-1638
    Abstract: This study aimed to evaluate changes in hormone secretion and in seminiferous epithelium of Angus bulls between 10 and 38 weeks of age. Samples of testicular parenchyma and blood were collected from 25 animals castrated in 4 week intervals. Traits associated to testicular development and quantitative aspects of spermatogenesis and hormonal concentrations were transformed by logarithm before analyses of variance. Changes in testis and seminiferous tubule diameter and testis weight were more pronounced after 26 weeks of age. The percentage of testicular parenchyma occupied by seminiferous tubules increased from 49.3 to 75.2% from 10 to 38 weeks. Most tubules ( 〉 90%) had only Sertoli cells at 10 and 14 weeks, but the number of tubules with gonocytes and A spermatogonia increased at 18 (13.8±1.7%) and 22 weeks (19±1%). Tubules with B and intermediate spermatogonia became predominant at 26 weeks (24.5±8.2%) and those with spermatocytes as the most advanced germ cell type were more evident at 30 weeks (42.3±9.9%). Round spermatids were detected at 26 weeks and at 38 weeks of age, 62.3±1.5% of all tubules had either elongate or mature spermatids. Variations in testis growth (specially testis weight) after 26 weeks were coincident with the establishment of meiosis in the seminiferous tubules, morphological alterations in nucleus and nucleolus of the Sertoli cells (indicators of Sertoli cell differentiation), lower levels of androstenedione and significant increases in testosterone and estradiol 17beta. Associations between testis development and concentrations of FSH and LH were less evident.
    Type of Medium: Online Resource
    ISSN: 1516-3598
    URL: Article
    DOI: 10.1590/S1516-35982006000600008
    Language: Unknown
    Publisher: FapUNIFESP (SciELO)
    Publication Date: 2006
    detail.hit.zdb_id: 2078814-9
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  • 5
    Online Resource
    Online Resource
    DESCRIÇÃO DO PERFIL PROTEÔMICO DO PLASMA SEMINAL DE ASININOS DA RAÇA PÊGA
    Universidade Federal do Tocantins ; 2019
    In:  DESAFIOS - Revista Interdisciplinar da Universidade Federal do Tocantins Vol. 6, No. 2 ( 2019-04-28), p. 3-9
    Details
    In: DESAFIOS - Revista Interdisciplinar da Universidade Federal do Tocantins, Universidade Federal do Tocantins, Vol. 6, No. 2 ( 2019-04-28), p. 3-9
    Abstract: A ausência da categoria asinina nas normas técnicas de avaliação seminal promove a necessidade de informações reprodutivas desta espécie. Objetivou-se descrever o perfil proteômico do plasma seminal de jumentos da raça Pêga (Equus asinus). Utilizaram-se seis animais púberes, com peso 239±32,6 Kg e circunferência escrotal de 36,8±6,9 cm, criados em mesma propriedade no estado do Tocantins, Brasil. Foi realizada eletroforese unidimensional, utilizando 12,5% de acrilamida e 30μg de proteína. As bandas foram descoradas e digeridas com tripsina para análise em espectrômetro de massa ESI-Q-TOF. Através de bioinformática, pelo banco de dados UniProtKB, as proteínas foram identificadas. Os termos da ontologia genética foram obtidos a partir do software STRAP®. A média da concentração proteica do plasma seminal foi 23,6±12,6 μg/μL. Foram detectadas pelo menos 26 bandas por animal (QuantityOne®). Um total de 19 bandas e 52 proteínas, com pesos entre 9,51 e 155,9 kDa, foram identificadas pela espectrometria. Os processos biológicos mais relevantes ligados às proteínas identificadas foram a regulação (24%) e processo celular (22%). As funções moleculares das proteínas foram descritas como ligação (42%) e atividade catalítica (31%). Em conclusão, a existência da descrição do padrão eletroforético destas proteínas plasmáticas seminais contribuirão com a construção de parâmetros para fertilidade.Palavras-chave: Proteômica, eletroforese, sêmen.
    Type of Medium: Online Resource
    ISSN: 2359-3652
    URL: Article
    DOI: 10.20873/uft.23593652201962p3
    Language: Unknown
    Publisher: Universidade Federal do Tocantins
    Publication Date: 2019
    detail.hit.zdb_id: 2857021-2
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  • 6
    Online Resource
    Online Resource
    H2O2 priming induces proteomic responses to defense against salt stress in maize
    Springer Science and Business Media LLC ; 2021
    In:  Plant Molecular Biology Vol. 106, No. 1-2 ( 2021-05), p. 33-48
    Details
    In: Plant Molecular Biology, Springer Science and Business Media LLC, Vol. 106, No. 1-2 ( 2021-05), p. 33-48
    Type of Medium: Online Resource
    ISSN: 0167-4412 , 1573-5028
    URL: Article
    DOI: 10.1007/s11103-021-01127-x
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2021
    detail.hit.zdb_id: 1475712-6
    SSG: 12
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  • 7
    Online Resource
    Online Resource
    HPV infection and 5mC/5hmC epigenetic markers in penile squamous cell carcinoma: new insights into prognostics
    Springer Science and Business Media LLC ; 2022
    In:  Clinical Epigenetics Vol. 14, No. 1 ( 2022-12)
    Details
    In: Clinical Epigenetics, Springer Science and Business Media LLC, Vol. 14, No. 1 ( 2022-12)
    Abstract: Penile cancer is one of the most aggressive male tumors. Although it is preventable, the main etiologic causes are lifestyle behaviors and viral infection, such as human papillomavirus (HPV). Long-term epigenetic changes due to environmental factors change cell fate and promote carcinogenesis, being an important marker of prognosis. We evaluated epidemiological aspects of penile squamous cell carcinoma (SCC) and the prevalence of HPV infection using high-risk HPV (hrHPV) and p16 INK4A expression of 224 participants. Global DNA methylation was evaluated through 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC). Results The incidence of HPV was 53.2% for hrHPV and 22.32% for p16 INK4a . hrHPV was not related to systemic or lymph node metastasis and locoregional recurrence, nor influenced the survival rate. P16 INK4a seems to be a protective factor for death, which does not affect metastasis or tumor recurrence. Lymph node and systemic metastases and locoregional recurrence increase the risk of death. An increased 5mC mark was observed in penile SCC regardless of HPV infection. However, there is a reduction of the 5hmC mark for p16 INK4a  + ( P  = 0.024). Increased 5mC/5hmC ratio ( 〉  1) was observed in 94.2% of penile SCC, irrespective of HPV infection. Despite the increase in 5mC, it seems not to affect the survival rate (HR = 1.06; 95% CI 0.33–3.38). Conclusions P16 INK4a seems to be a good prognosis marker for penile SCC and the increase in 5mC, an epigenetic mark of genomic stability, may support tumor progression leading to poor prognosis.
    Type of Medium: Online Resource
    ISSN: 1868-7075 , 1868-7083
    URL: Article
    DOI: 10.1186/s13148-022-01360-1
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2022
    detail.hit.zdb_id: 2553921-8
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  • 8
    Online Resource
    Online Resource
    Utilização do farelo de castanha de caju na terminação de ovinos em confinamento
    FapUNIFESP (SciELO) ; 2003
    In:  Revista Brasileira de Zootecnia Vol. 32, No. 1 ( 2003-02), p. 240-248
    Details
    In: Revista Brasileira de Zootecnia, FapUNIFESP (SciELO), Vol. 32, No. 1 ( 2003-02), p. 240-248
    Abstract: A study was conducted to evaluate the use of cashew nuts meal (CNM) in diets for sheep, raised in feedlot. Thirty two crossbred animals received concentrates with the same amount of crude protein, but four different levels of CNM (0, 12, 24 and 36%). A factorial 4 x 2 was used, with four levels of CNM and 2 sexes, with four repetitions. Daily weight gain and feed conversion as well as intake of dry matter (DM), crude protein (CP), ether extract (EE), neutral detergent fiber(NDF) and ashes were determined by analysis of variance, Duncan statistical test and regression analysis. Intake was estimated as g/animal/day, percentage of body weight (%BW) and g/BW0.75. There were differences in intake of DM, CP and EE when animals were fed concentrates with 0 and 36% of CNM. Also, intake of DM, CP and NDF tended to decrease as the levels of CNM increased in the diet. EE intake increased according to the different CNM levels. The type of diet did not significantly affect either weight gain or feed conversion. Therefore, cashew nut meal can be included up to 24% in the concentrate, but the total diets should not have more than 6% of fat.
    Type of Medium: Online Resource
    ISSN: 1516-3598
    URL: Article
    DOI: 10.1590/S1516-35982003000100030
    Language: Unknown
    Publisher: FapUNIFESP (SciELO)
    Publication Date: 2003
    detail.hit.zdb_id: 2078814-9
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  • 9
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    Online Resource
    First proteomic analysis of diestrus and anestrus canine oocytes at the germinal vesicle reveals candidate proteins involved in oocyte meiotic competence
    Wiley ; 2019
    In:  Reproduction in Domestic Animals Vol. 54, No. 12 ( 2019-12), p. 1532-1542
    Details
    In: Reproduction in Domestic Animals, Wiley, Vol. 54, No. 12 ( 2019-12), p. 1532-1542
    Abstract: In domestic dogs, oocyte maturation rates are low and the percentage of oocytes that remain in the stage of germinal vesicle (GV) regardless of culture conditions is high. The present study was conducted to characterize the proteome of canine oocyte at the germinal vesicle stage using label‐free mass spectrometry. Ovaries were collected from 415 adult domestic dogs and oocytes were divided anestrus and diestrus group. Protein lysates were subjected to quantitative proteomic analysis to identify differentially expressed proteins in different status reproductive. All runs for each sample were performed on an Easy nLC1000 nano‐LC chromatograph system directly connected to a quadrupole‐type Orbitrap mass spectrometer. For identification of peptides and proteins, raw data of the spectra were loaded into MaxQuant software version 1.5.2.8. Proteomic data were analysed according to gene ontology and a protein–protein interaction network. 312 proteins were identified and grouped according to their biological processes, molecular functions and cellular component. Forty‐six differentially expressed proteins among diestrus and control group were associated with at least one GO term in the biological process database. Several proteins involved in the cell cycle, fertilization, regulation of transcription and signalling pathways that are essential for the full development of oocytes and fertilization were expressed. This study identified proteins that were absent, and more or less expressed in different status reproductive. These differentially expressed proteins revealed a framework of molecular reorganization within a GV that renders its competency. This knowledge will enable the identification of target competence biomarkers and thus the establishment of more adequate means of cultivation to improve the M‐I and II indexes in this species and also to better understand the physiology of the domestic dog, promoting the development of new reproduction biotechniques.
    Type of Medium: Online Resource
    ISSN: 0936-6768 , 1439-0531
    URL: Issue
    URL: Article
    DOI: 10.1111/rda.v54.12
    DOI: 10.1111/rda.13560
    Language: English
    Publisher: Wiley
    Publication Date: 2019
    detail.hit.zdb_id: 2020494-2
    SSG: 12
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  • 10
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    Online Resource
    Feeding effects of cottonseed and its co-products on the meat proteome from ram lambs
    FapUNIFESP (SciELO) ; 2019
    In:  Scientia Agricola Vol. 76, No. 6 ( 2019-12), p. 463-472
    Details
    In: Scientia Agricola, FapUNIFESP (SciELO), Vol. 76, No. 6 ( 2019-12), p. 463-472
    Type of Medium: Online Resource
    ISSN: 1678-992X
    URL: Article
    DOI: 10.1590/1678-992x-2018-0072
    Language: English
    Publisher: FapUNIFESP (SciELO)
    Publication Date: 2019
    detail.hit.zdb_id: 2016347-2
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