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  • 1
    Online Resource
    Online Resource
    American Society for Microbiology ; 1978
    In:  Applied and Environmental Microbiology Vol. 36, No. 3 ( 1978-09), p. 506-512
    In: Applied and Environmental Microbiology, American Society for Microbiology, Vol. 36, No. 3 ( 1978-09), p. 506-512
    Abstract: A technique is described for the determination of bacterial numbers and the spectrum of actively metabolizing cells on the same microscopic preparation by a combined autoradiography/epifluorescence microscopy technique. Natural bacterial populations incubated with [ 3 H]glucose were filtered onto 0.2-μm Nuclepore polycarbonate membranes. The filters were cut into quarters and fixed on the surface of glass slides, coated with NTB-2 nuclear track emulsion (Kodak), and exposed to the radiation. After processing, the autoradiographs were stained with acridine orange. A combination of overstaining on the slightly alkaline side and gradual destaining on the acid side of neutrality gave the best results. Epifluorescence microscopy revealed br ight-orange fluorescent cells with dark-silver grains associated against a greenish-to-grayish background. Based on the standardization curves, detection of actually metabolizing cells was optimal when cells were incubated with 1 to 5 μCi of [ 3 H]glucose per ml of sample for 4 h and the autoradiographs were exposed to NTB-2 emulsion at 7°C for 3 days. In water samples taken immediately above sandy sediments at beaches of the Kiel Fjord and the Kiel Bight (Baltic Sea, FRG), between 2.3 and 56.2% (average, 31.3%) of the total number of bacteria were actually metabolizing cells. Spearman rank correlation analysis revealed significant interrelationships between the number of active bacteria and the actual uptake rate of glucose.
    Type of Medium: Online Resource
    ISSN: 0099-2240 , 1098-5336
    RVK:
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 1978
    detail.hit.zdb_id: 223011-2
    detail.hit.zdb_id: 1478346-0
    SSG: 12
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  • 2
    Online Resource
    Online Resource
    Elsevier BV ; 2000
    In:  Marine Pollution Bulletin Vol. 41, No. 1-6 ( 2000-1), p. 255-263
    In: Marine Pollution Bulletin, Elsevier BV, Vol. 41, No. 1-6 ( 2000-1), p. 255-263
    Type of Medium: Online Resource
    ISSN: 0025-326X
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2000
    detail.hit.zdb_id: 414337-1
    detail.hit.zdb_id: 2001296-2
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  • 3
    Online Resource
    Online Resource
    American Society for Microbiology ; 1987
    In:  Applied and Environmental Microbiology Vol. 53, No. 8 ( 1987-08), p. 1748-1755
    In: Applied and Environmental Microbiology, American Society for Microbiology, Vol. 53, No. 8 ( 1987-08), p. 1748-1755
    Abstract: Seasonal and spatial distributions of extracellular enzymatic activities and microbial incorporations of dissolved organic substrates were followed in sediments of the brackish water Kiel Bight (Baltic Sea, Federal Republic of Germany). Enzymatic hydrolysis of polymeric organic compounds was determined by means of fluorogenic substrates (4-methylumbelliferyl-β- d -glucoside, l -leucine-4-methylcoumarinyl-7-amide hydrochloride); incorporation of dissolved organic substrates into microbial biomass was measured by using tritiated substances (acetate, leucine, and thymidine). Based on a recently developed core injection technique, substrates were injected in microliter portions into undisturbed sediment cores. Enzymatic and incorporation activities underwent strong seasonal variations related to the enrichment of organic material in the sediment surface following sedimentation events. The input of the phytoplankton bloom during autumn caused stimulation of both enzymatic hydrolysis of polymeric organic compounds and microbial incorporation of dissolved organic substrates. Following input by spring phytoplankton bloom, mainly incorporation activities were stimulated. In late spring the development of the benthic fauna obviously greatly influenced microbial activities. During summer individual periods of high microbial activities were observed which might be traced back to short-term sedimentation events. The high microbial incorporation of leucine and thymidine during winter demonstrated that the nutrient supply rather than temperature is the dominating factor determining microbial production. Stimulation of microbial activities arose from the sediment surface and spread out relatively quickly into deeper horizons. Generally, the sediments were characterized by distinct patterns of interrelationships between the individual parameters of microbial activities measured.
    Type of Medium: Online Resource
    ISSN: 0099-2240 , 1098-5336
    RVK:
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 1987
    detail.hit.zdb_id: 223011-2
    detail.hit.zdb_id: 1478346-0
    SSG: 12
    Location Call Number Limitation Availability
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  • 4
    In: Estuarine, Coastal and Shelf Science, Elsevier BV, Vol. 13, No. 5 ( 1981-11), p. 579-589
    Type of Medium: Online Resource
    ISSN: 0272-7714
    Language: English
    Publisher: Elsevier BV
    Publication Date: 1981
    detail.hit.zdb_id: 1466742-3
    detail.hit.zdb_id: 763369-5
    SSG: 21,3
    SSG: 12
    SSG: 14
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  • 5
    In: International Review of Hydrobiology, Wiley, Vol. 83, No. 5-6 ( 1998-01), p. 479-499
    Abstract: The Nordrügenschen Bodden waters are a shallow coastal inlet of the Southern Baltic Sea consisting of individual basins, some of which are joined only via small connections. The Bodden are characterized by pronounced horizontal gradients in hydrographical, chemical and biological parameters. Whereas concentrations of inorganic nutrients increase, salinity decreases with increasing distance from the connection to the Baltic Sea. Between 1960 and 1989 long‐term variations of salinity, inorganic nutrients (ammonium, nitrate, phosphate), Secchi disk visibility, and planktonic primary production were monitored at 6 locations throughout the Bodden. The gradients were most pronounced during the first period of observation (1960–1963). Between 1960 and 1985 concentrations of inorganic nutrients in the adjacent Arkona Sea increased up to values several times higher than the previous concentrations. This increased nutrient load in the Baltic Sea was reflected by higher nutrient concentrations and higher primary production in the outer and central parts of the Nordrügenschen Bodden. Between 1963 and 1965 the shipping channel between the outer (Libben) and the inner part (Großer Jasmunder Bodden) of the Bodden chain was dredged. This led to an improvement of water exchange between the Baltic Sea and the Bodden, resulting in a significant reduction of the gradients of chemical and biological parameters. Whereas salinity and Secchi disk visibility increased, inorganic nutrients, and primary production decreased. This was most obvious in the inner parts of the Bodden where the water quality was improved. The trophic status of the Kleiner Jasmunder Bodden is still worrying. This Bodden represents the innermost part of the Nordrügenschen Bodden connected with the Großer Jasmunder Bodden only via a small flood gate. Unless the water exchange is drastically improved, the Kleiner Jasmunder Bodden will retain its hypertrophic status in the longer term. The future development of the trophic status of the Bodden is discussed.
    Type of Medium: Online Resource
    ISSN: 1434-2944 , 1522-2632
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 1998
    detail.hit.zdb_id: 2006634-X
    detail.hit.zdb_id: 1420232-3
    SSG: 12
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