GLORIA

GEOMAR Library Ocean Research Information Access

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    facet.materialart.
    Unknown
    Metabarcoding read abundances of orchid mycorrhizal fungi are correlated to copy numbers estimated using ddPCR (2023)
    Wiley
    In:  New Phytologist vol. 2023
    Details
    Publication Date: 2024-01-22
    Description: Quantifying the abundances of fungi is key to understanding natural variation in mycorrhi-zal communities in relation to plant ecophysiology and environmental heterogeneity. High-throughput metabarcoding approaches have transformed our ability to characterize and com-pare complex mycorrhizal communities. However, it remains unclear how well metabarcodingread counts correlate with actual read abundances in the sample, potentially limiting their useas a proxy for species abundances. Here, we use droplet digital PCR (ddPCR) to evaluate the reliability of ITS2 metabarcodingdata for quantitative assessments of mycorrhizal communities in the orchid speciesNeottiaovatasampled at multiple sites. We performed specific ddPCR assays for eight families oforchid mycorrhizal fungi and compared the results with read counts obtained from metabar-coding. Our results demonstrate a significant correlation between DNA copy numbers measured byddPCR assays and metabarcoding read counts of major mycorrhizal partners ofN. ovata,highlighting the usefulness of metabarcoding for quantifying the abundance of orchid mycor-rhizal fungi. Yet, the levels of correlation between the two methods and the numbers of falsezero values varied across fungal families, which warrants cautious evaluation of the reliabilityof low-abundance families. This study underscores the potential of metabarcoding data for more quantitative analysesof mycorrhizal communities and presents practical workflows for metabarcoding and ddPCRto achieve a more comprehensive understanding of orchid mycorrhizal communities
    Keywords: droplet digital PCR ; fungalquantification ; metabarcoding ; mycorrhizalfungi ; orchid mycorrhiza
    Repository Name: National Museum of Natural History, Netherlands
    Type: info:eu-repo/semantics/article
    Format: application/pdf
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    PAPER (OA)
  • 2
    facet.materialart.
    Unknown
    Dispatch from the field: ecology of ground-web-building spiders with description of a new species (Araneae, Symphytognathidae) (2014)
    Pensoft Publishers
    In:  Biodiversity Data Journal vol. 2, pp. e1076-e1076
    Details
    Publication Date: 2024-01-12
    Keywords: Ecology ; Ecology ; Evolution ; Behavior and Systematics
    Repository Name: National Museum of Natural History, Netherlands
    Type: info:eu-repo/semantics/article
    Format: application/pdf
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    PAPER (OA)
  • 3
    facet.materialart.
    Unknown
    Comparative Pollen Development in Dioscoreales (2005)
    In:  International Journal of Plant Sciences vol. 166 no. 6, pp. 909-924
    Details
    Publication Date: 2024-01-12
    Description: Pollen and orbicule ontogeny in representatives of three genera of Dioscoreales\xe2\x80\x94Narthecium ossifragum Huds. (Nartheciaceae), Tacca artocarpifolia Seem. and Tacca chantrieri Andre\xc2\xb4 (Dioscoreaceae), and Dioscorea communis (L.) Caddick & Wilkin (Dioscoreaceae)\xe2\x80\x94is described and illustrated using LM, SEM, and TEM. The main difference is in microsporogenesis, which is successive in Narthecium Huds. and simultaneous in Tacca J.R. & G. Forst. and Dioscorea L. This is reflected in the tetrad configuration but not in the apertures: Narthecium and Tacca are monosulcate, while Dioscorea has two equatorial apertures. Other features of pollen development are similar in all three genera. Exine development starts at the proximal pole, and during the tetrad stage, the exine remains thicker at this pole. Intine development begins before mitosis. A conspicuously channeled intine forms beneath the aperture(s) in all three genera, and in Tacca it also occurs in nonapertural regions. After mitosis, the generative cell is situated at the proximal pole in all genera. Orbicules are present in Narthecium and Dioscorea but not in Tacca, although it has a secretory tapetum. In the tetrad stage, pro-orbicules appear on the tapetum in Narthecium and Dioscorea, and pro-sporopollenin is consequently deposited to form the orbicule wall.
    Keywords: intine ; microsporogenesis ; monocotyledons ; orbicules ; pollen ontogeny ; systematics ; tapetum ; tetrads
    Repository Name: National Museum of Natural History, Netherlands
    Type: info:eu-repo/semantics/article
    Format: application/pdf
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    PAPER (OA)
  • 4
    facet.materialart.
    Unknown
    Thismia (Thismiaceae): the first record of the mycoheterotrophic genus to the Flora of India with a new species revealing the phytogeographical significance of Western Ghats (2017)
    In:  Blumea: Biodiversity, Evolution and Biogeography of Plants vol. 62, pp. 97-102
    Details
    Publication Date: 2024-01-12
    Description: Thismia, a genus of mycoheterotrophic plants, is reported for the first time from mainland India, from Neryamangalam forests in Idukki district of Kerala, along with a new species, T. sahyadrica. Thismia sahyadrica, described and illustrated here, is unique within Thismia in having a mitre with a single opening; five perianth lobes are fused into a mitre-like structure, while the sixth one is free, forming a lateral single opening of the flower. Due to its unique morphological characteristics, the taxonomic placement of the new species remains obscure, although some root and flower characters suggest an affinity with species from the sections Glaziocharis, Sarcosiphon, Geomitra, and Scaphiophora. Ecological specificity and phytogeographical peculiarities of the new species are also discussed.
    Keywords: Kerala ; mycoheterotrophy ; Thismia ; Western Ghats
    Repository Name: National Museum of Natural History, Netherlands
    Type: info:eu-repo/semantics/article
    Format: application/pdf
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    PAPER (OA)
  • 5
    facet.materialart.
    Unknown
    Phylogeny and evolution of Burmanniaceae (Dioscoreales) based on nuclear and mitochondrial data (2006)
    In:  American Journal of Botany vol. 93 no. 11, pp. 1684-1698
    Details
    Publication Date: 2024-01-12
    Description: The mycoheterotrophic Burmanniaceae are one of the three families currently recognized in the order Dioscoreales. Phylogenetic inference using nucleotide sequences of the nuclear 18S rDNA region and the mitochondrial nad1 b-c intron revealed two well-supported, major lineages within the family, corresponding to the two tribes recognized in the family: Burmannieae and Thismieae. All data supported a strong relationship between Thismieae and Tacca (Dioscoreaceae) making both Burmanniaceae and Dioscoreaceae polyphyletic. The three largest Burmanniaceae genera, Burmannia, Gymnosiphon, and Thismia, are paraphyletic. The splitting of Burmanniaceae into Burmannieae and Thismieae indicates two independent origins of mycoheterotrophy and correlated loss of chlorophyll in Dioscoreales. In the genus Burmannia, in which many species still contain chlorophyll, the achlorophyllous species are nested in between the autotrophic species, suggesting many independent changes from autotrophy to heterotrophy or vice versa. A Bayesian relative rates test on the 18S rDNA data showed considerable variation in substitution rates among Burmanniaceae. The substitution rates in all Thismieae and many Burmannieae are significantly faster than in Dioscoreaceae, but there seems to be no correlation between rate increases and the loss of photosynthesis.
    Keywords: 18S rDNA ; Burmanniaceae ; Dioscoreales ; molecular phylogeny ; mycoheterotrophy ; nad1 b-c intron ; Thismiaceae
    Repository Name: National Museum of Natural History, Netherlands
    Type: info:eu-repo/semantics/article
    Format: application/pdf
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    PAPER (OA)
  • 6
    facet.materialart.
    Unknown
    Phylogenetic relationships in Nartheciaceae (Dioscoreales), with focus on pollen and orbicule morphology (2008)
    In:  Belgian Journal of Botany vol. 141 no. 1, pp. 64-77
    Details
    Publication Date: 2024-01-12
    Description: Amolecular phylogenetic analysis of Nartheciaceae is presented, with nine species of the family\xe2\x80\x99s five genera. The main phylogenetic findings are: (a) Nietneria and Narthecium are placed in a clade sister to Lophiola; (b) sister to the Lophiola-Narthecium- Nietneria clade is a clade formed by Aletris and the monospecific Metanarthecium; (c) the inclusion of Metanarthecium luteo-viride in Aletris, as proposed by several authors, is well supported. The pollen and orbicule morphology of representatives of five genera is described. The results underline a close relationship between Nietneria, Narthecium, and Lophiola and confirm the previously reported observations of Metanarthecium pollen and the types of sexine ornamentation in Aletris. Pollen grains of Nietneria are monosulcate with a microreticulate sexine, confirming a close relationship with Lophiola and Narthecium. Spherical smoothsurfaced orbicules were observed in all genera of Nartheciaceae and the presence of a circular perforation on the orbicule surface is potentially synapomorphic for the family.
    Keywords: Dioscoreales ; Nartheciaceae ; Nietneria ; molecular phylogeny ; orbicules ; pollen morphology
    Repository Name: National Museum of Natural History, Netherlands
    Type: info:eu-repo/semantics/article
    Format: application/pdf
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    PAPER (OA)
  • 7
    facet.materialart.
    Unknown
    Diversification of myco-heterotrophic angiosperms: evidence from Burmanniaceae (2008)
    In:  BMC Evolutionary Biology vol. 8 no. 178
    Details
    Publication Date: 2024-01-12
    Description: Background: Myco-heterotrophy evolved independently several times during angiosperm evolution. Although many species of myco-heterotrophic plants are highly endemic and longdistance dispersal seems unlikely, some genera are widely dispersed and have pantropical distributions, often with large disjunctions. Traditionally this has been interpreted as evidence for an old age of these taxa. However, due to their scarcity and highly reduced plastid genomes our understanding about the evolutionary histories of the angiosperm myco-heterotrophic groups is poor. \nResults: We provide a hypothesis for the diversification of the myco-heterotrophic family Burmanniaceae. Phylogenetic inference, combined with biogeographical analyses, molecular divergence time estimates, and diversification analyses suggest that Burmanniaceae originated in West Gondwana and started to diversify during the Late Cretaceous. Diversification and migration of the species-rich pantropical genera Burmannia and Gymnosiphon display congruent patterns. Diversification began during the Eocene, when global temperatures peaked and tropical forests occurred at low latitudes. Simultaneous migration from the New to the Old World in Burmannia and Gymnosiphon occurred via boreotropical migration routes. Subsequent Oligocene cooling and breakup of boreotropical flora ended New-Old World migration and caused a gradual decrease in diversification rate in Burmanniaceae. \nConclusion: Our results indicate that extant diversity and pantropical distribution of mycoheterotrophic Burmanniaceae is the result of diversification and boreotropical migration during the Eocene when tropical rain forest expanded dramatically.
    Keywords: myco-heterotrophy ; angiosperm evolution ; pantropical distribution ; reduced plastid genomes ; evolutionary history ; Burmanniaceae ; West Gondwana ; diversification ; boreotropical migration ; Eocene
    Repository Name: National Museum of Natural History, Netherlands
    Type: info:eu-repo/semantics/article
    Format: application/pdf
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    PAPER (OA)
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...