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  • 1
    Publication Date: 2011-06-08
    Description: Mycoplasma parasites escape host immune responses via mechanisms that depend on remarkable phenotypic plasticity. Identification of these mechanisms is of great current interest. The aminoacyl-tRNA synthetases (AARSs) attach amino acids to their cognate tRNAs, but occasionally make errors that substitute closely similar amino acids. AARS editing pathways clear errors to avoid mistranslation during protein synthesis. We show here that AARSs in Mycoplasma parasites have point mutations and deletions in their respective editing domains. The deleterious effect on editing was confirmed with a specific example studied in vitro. In vivo mistranslation was determined by mass spectrometric analysis of proteins produced in the parasite. These mistranslations are uniform cases where the predicted closely similar amino acid replaced the correct one. Thus, natural AARS editing-domain mutations in Mycoplasma parasites cause mistranslation. We raise the possibility that these mutations evolved as a mechanism for antigen diversity to escape host defense systems.
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
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  • 2
    Publication Date: 2013-03-06
    Description: Mycoplasma leucyl-tRNA synthetases (LeuRSs) have been identified in which the connective polypeptide 1 (CP1) amino acid editing domain that clears mischarged tRNAs are missing (Mycoplasma mobile) or highly degenerate (Mycoplasma synoviae). Thus, these enzymes rely on a clearance pathway called pretransfer editing, which hydrolyzes misactivated aminoacyl-adenylate intermediate via a nebulous...
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
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  • 3
    Publication Date: 2020-02-12
    Description: Earth observation satellites yield a wealth of data for scientific, operational and commercial exploitation. However, the redistribution of mass in the system Earth is not yet part of the standard inventory of Earth Observation (EO) data products to date. It is derived from the Gravity Recovery and Climate Experiment (GRACE) mission and its Follow-On mission (GRACE-FO). Among many other applications, mass redistribution provides fundamental insights into the global water cycle. Changes in continental water storage impact the regional water budget and can, in extreme cases, result in floods and droughts that often claim a high toll on infrastructure, economy and human lives. The initiative for a European Gravity Service for Improved Emergency Management (EGSIEM) established three different prototype services to promote the unique value of mass redistribution products for Earth Observation in general and for early-warning systems in particular. The first prototype service is a scientific combination service to derive improved mass redistribution products from the combined knowledge of the European GRACE analysis centres. Secondly, the timeliness and reliability of such products is a primary concern for any early-warning system and therefore EGSIEM established a prototype for a near real-time service that provides dedicated gravity field information with a maximum latency of five days . Third, EGSIEM established a prototype of a hydrological / early warning service that derives wetness indices as indicators of hydrological extremes and assessed their potential for timely scheduling of high-resolution optical/radar satellites for follow-up observations in case of evolving hydrological extreme events.
    Type: info:eu-repo/semantics/article
    Format: application/pdf
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  • 4
    Publication Date: 2020-02-12
    Description: In this paper, an automatic near-real time (NRT) flood detection approach is presented, which combines histogram thresholding and segmentation based classification, specifically oriented to the analysis of single-polarized very high resolution Synthetic Aperture Radar (SAR) satellite data. The challenge of SAR-based flood detection is addressed in a completely unsupervised way, which assumes no training data and therefore no prior information about the class statistics to be available concerning the area of investigation. This is usually the case in NRT-disaster management, where the collection of ground truth information is not feasible due to time-constraints. A simple thresholding algorithm can be used in the most of the cases to distinguish between "flood" and "non-flood" pixels in a high resolution SAR image to detect the largest part of an inundation area. Due to the fact that local gray-level changes may not be distinguished by global thresholding techniques in large satellite scenes the thresholding algorithm is integrated into a split-based approach for the derivation of a global threshold by the analysis and combination of the split inherent information. The derived global threshold is then integrated into a multi-scale segmentation step combining the advantages of small-, medium- and large-scale per parcel segmentation. Experimental investigations performed on a TerraSAR-X Stripmap scene from southwest England during large scale flooding in the summer 2007 show high classification accuracies of the proposed split-based approach in combination with image segmentation and optional integration of digital elevation models.
    Type: info:eu-repo/semantics/article
    Format: application/pdf
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  • 5
    Publication Date: 2020-02-12
    Description: In this contribution, a hybrid multi-contextual Markov model for unsupervised near real-time flood detection in multi-temporal X-band synthetic aperture radar (SAR) data is presented. It incorporates scale-dependent, as well as spatio-temporal contextual information, into the classification scheme, by combining hierarchical marginal posterior mode (HMPM) estimation on directed graphs with noncausal Markov image modeling related to planar Markov random fields (MRFs). In order to increase computational performance, marginal posterior-based entropies are used for restricting the iterative bi-directional exchange of spatio-temporal information between consecutive images of a time sequence to objects exhibiting a low probability, to be classified correctly according to the HMPM estimation. The Markov models, originally developed for inference on regular graph structures of quadtrees and planar lattices, are adapted to the variable nature of irregular graphs, which are related to information driven image segmentation. Entropy based confidence maps, combined with spatio-temporal relationships of potentially inundated bright scattering vegetation to open water areas, are used for the quantification of the uncertainty in the labeling of each image element in flood possibility masks. With respect to accuracy and computational effort, experiments performed on a bi-temporal TerraSAR-X ScanSAR data-set from the Caprivi region of Namibia during flooding in 2009 and 2010 confirm the effectiveness of integrating hierarchical as well as spatio-temporal context into the labeling process, and of adapting the models to irregular graph structures.
    Type: info:eu-repo/semantics/article
    Format: application/pdf
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  • 6
    Publication Date: 2020-02-12
    Description: The near real-time provision of precise information about flood dynamics from synthetic aperture radar (SAR) data is an essential task in disaster management. A novel tile-based parametric thresholding approach under the generalized Gaussian assumption is applied on normalized change index data to automatically solve the three-class change detection problem in large-size images with small class a priori probabilities. The thresholding result is used for the initialization of a hybrid Markov model which integrates scale-dependent and spatiocontextual information into the labeling process by combining hierarchical with noncausal Markov image modeling. Hierarchical maximum a posteriori (HMAP) estimation using the Markov chains in scale, originally developed on quadtrees, is adapted to hierarchical irregular graphs. To reduce the computational effort of the iterative optimization process that is related to noncausal Markov models, a Markov random field (MRF) approach is defined, which is applied on a restricted region of the lowest level of the graph, selected according to the HMAP labeling result. The experiments that were performed on a bitemporal TerraSAR-X StripMap data set from South West England during and after a large-scale flooding in 2007 confirm the effectiveness of the proposed change detection method and show an increased classification accuracy of the hybrid MRF model in comparison to the sole application of the HMAP estimation. Additionally, the impact of the graph structure and the chosen model parameters on the labeling result as well as on the performance is discussed.
    Language: English
    Type: info:eu-repo/semantics/article
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  • 7
    Publication Date: 2020-02-12
    Language: English
    Type: info:eu-repo/semantics/conferenceObject
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  • 8
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    In:  EUSAR 2008 : June 2 - 5, 2008, Friedrichshafen, Germany
    Publication Date: 2020-02-12
    Description: Medium resolution SAR satellite data have been widely used for water and flood mapping in recent years. Since 2007 high resolution radar data with up to 1 m pixel spacing of the TerraSAR-X satellite are operationally available. The improved ground resolution of the system offers enormous potential for water detection. However, image analysis gets more challenging due to the large amount of image objects that are visible in the data. Water body detection methods are reviewed with regard to their applicability for TerraSAR-X data. Flood detection approaches for rapid disaster mapping are presented in this paper.
    Language: English
    Type: info:eu-repo/semantics/conferenceObject
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  • 9
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    In:  Geographic Information and Cartography for Risk and Crisis Management : Towards Better Solutions | Lecture Notes in Geoinformation and Cartography
    Publication Date: 2020-02-12
    Language: English
    Type: info:eu-repo/semantics/bookPart
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  • 10
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    In:  Proceedings / 2008 IEEE International Geoscience & Remote Sensing Symposium : July 6 - 11, 2008
    Publication Date: 2020-02-12
    Language: English
    Type: info:eu-repo/semantics/conferenceObject
    Format: application/pdf
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